LOCUS       sequence-241745-       10202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of HTATSF1__BRS3 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-HTATSF1__BRS3
FEATURES             Location/Qualifiers
     source          1..10202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5113..5136
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(5139..5163)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5216..5257
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5278..5845
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5295..5847
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5439..5456)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5666..5685
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5859..5861,5862..5864,5865..6578)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5859..5861,5862..5864,5865..6575)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5904..5925)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6165..6184)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6512..6533
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6592..7180
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6645..6665)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7063..7074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7255..7488
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7607..7625)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7693..7797
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7798..7866,7867..8658)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8016..8035)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8829..9417
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9318..9337
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9571..9588
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9828..9849
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9864..9869,9870..9887,9888..9894)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9902..9918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9907..9929
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9926..9942
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9926..9942
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9992..10011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatgagc ggcaccaacc tggacggcaa cgacgagttc
     3481 gacgagcagc tgcggatgca ggaactgtac ggcgacggca aggatggcga cacccagaca
     3541 gatgccggcg gagagcctga ttctctgggc cagcagccca ccgacacccc ttacgagtgg
     3601 gacctggaca agaaggcctg gttccccaag atcaccgagg actttatcgc cacctaccag
     3661 gccaactacg gcttctccaa cgacggcgcc agctccagca ccgccaacgt ggaagatgtg
     3721 cacgccagaa ccgccgagga acctccccag gaaaaggccc ctgagcctac cgatgcccgc
     3781 aagaagggcg agaagagaaa ggccgagagc ggctggttcc atgtggaaga ggaccggaac
     3841 accaacgtgt acgtgtccgg cctgcctccc gacatcaccg tggatgagtt catccagctg
     3901 atgagcaagt tcggcatcat catgcgggac cctcagaccg aggaattcaa agtgaagctg
     3961 tacaaggaca accagggcaa cctgaaaggc gacggactgt gctgctacct gaagcgcgag
     4021 agcgtggaac tggccctgaa gctgctggac gaggacgaga tccggggcta caaactgcac
     4081 gtcgaggtgg ccaagtttca gctcaagggc gaatacgacg ccagcaagaa aaagaagaag
     4141 tgcaaggact acaaaaagaa actgagcatg cagcagaagc agctcgactg gcggcctgag
     4201 agaagggccg gacccagcag aatgcggcac gagcgggtgg tcatcatcaa gaatatgttc
     4261 caccccatgg acttcgagga tgaccctctg gtgctgaacg agatcagaga ggacctgcgg
     4321 gtcgagtgca gcaagtttgg ccagatccgg tacaaggccg tggtcaagcc cctggaaaga
     4381 cagcccagca atgccatcct gaaaacctgc gtgaaggccg gctgcgtgtg gatcgtgtcc
     4441 atgatctttg ccctgcccga ggccatcttc agcaatgtgt acaccttccg ggaccccaac
     4501 aagaacatga ccttcgagag ctgcaccagc taccccgtgt ccaaaaaact gctgcaggaa
     4561 atccacagcc tgctctgctt tctggtgttc tacatcatcc ctctgagcat catctccgtg
     4621 tactacagcc tgatcgcccg gaccctgtat aagagcaccc tgaacatccc cacagaggaa
     4681 cagagccacg cccggaagca gatcgagagc cggaagagaa tcgccaggac cgtgctggtc
     4741 ctggtggccc tgtttgctct gtgctggctg cccaaccatc tgctgtacct gtaccactcc
     4801 ttcaccagcc agacctacgt ggaccccagc gccatgcact ttatcttcac catcttctcc
     4861 cgggtgctgg cctttagcaa cagctgtgtg aaccccttcg ccctgtactg gctgagcaag
     4921 agcttccaga agcacttcaa ggcccagctg ttctgttgca aggccgaaag acccgagcct
     4981 cccgtggccg ataccagcct gacaacactg gccgtgatgg gcacagtgcc aggcaccggc
     5041 agcatccaga tgagcgagat ctctgtgacc agctttaccg gctgcagcgt gaagcaggcc
     5101 gaggacagat tcgactacaa ggatgacgat gacaagtgac ccaactttct tgtacaaagt
     5161 ggttgatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa
     5221 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagatcc
     5281 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     5341 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     5401 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     5461 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     5521 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     5581 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     5641 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     5701 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     5761 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     5821 tttcctttga aaaacacgat gataatatgg ccacacatat ggtgagcaag ggcgaggagc
     5881 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     5941 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     6001 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     6061 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     6121 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     6181 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     6241 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     6301 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     6361 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     6421 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     6481 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     6541 ccgggatcac tctcggcatg gacgagctgt acaagtaaat cgatagatcc taatcaacct
     6601 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6661 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6721 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6781 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6841 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6901 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6961 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7021 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7081 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7141 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
     7201 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7261 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     7321 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7381 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7441 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     7501 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     7561 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc
     7621 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     7681 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7741 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7801 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7861 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7921 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7981 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8041 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8101 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8161 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8221 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8281 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8341 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8401 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8461 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8521 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8581 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8641 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8701 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8761 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8821 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8881 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8941 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9001 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9061 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9121 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9181 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9241 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9301 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9361 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9421 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9481 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9541 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9601 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc
     9661 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     9721 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg
     9781 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     9841 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     9901 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca
     9961 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10021 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    10081 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    10141 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    10201 gt
//
