LOCUS       sequence-241750-       11572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of STK31.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-STK31
FEATURES             Location/Qualifiers
     source          1..11572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6509..6533)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6586..6627
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6648..7215
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6665..7217
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6809..6826)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7036..7055
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7229..7231,7232..7234,7235..7948)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7229..7231,7232..7234,7235..7945)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7274..7295)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7535..7554)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7882..7903
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7962..8550
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8015..8035)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8433..8444)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8625..8858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8977..8995)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9063..9167
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9168..9236,9237..10028)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9386..9405)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10199..10787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10688..10707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10941..10958
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11198..11219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11234..11239,11240..11257,11258..11264)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11272..11288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11277..11299
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11296..11312
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11296..11312
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11362..11381
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg tgggtccagg gtcactcttc tagagcttcc
     3481 gcaacggaaa gtgtgagttt ttcaggaatt gttcaaatgg atgaagatac acattacgat
     3541 aaagtggaag atgtggttgg aagtcacata gaagatgcag taacattttg ggcccagagt
     3601 atcaatagaa ataaggatat catgaagatt ggttgctcac tgtctgaagt ttgcccccac
     3661 gccagttcag ttttggggaa tcttgaccca aacaagattt atggtggatt attttctgaa
     3721 gatcagtgtt ggtacagatg caaagtactg aaaatcatca gcgttgaaaa gtgtctggtg
     3781 aggtacattg actatggaaa tactgaaatt ctaaatcgat ctgatatagt tgaaattcct
     3841 ttggagctgc agttttctag tgttgccaaa aagtataaac tttggggact acacattcct
     3901 tctgatcaag aagttaccca gtttgatcag ggcacaacct ttttggggag cttgattttt
     3961 gaaaaggaaa taaaaatgag aattaaagca acctctgaag atggaacagt tattgctcag
     4021 gctgagtatg gcagtgtgga tataggggaa gaggtgctta agaaaggatt tgcagagaaa
     4081 tgcagacttg cttccagaac tgacatctgt gaggaaaaaa aattggatcc tggtcaactt
     4141 gttctcagga acctcaaaag ccccattcct ttgtgggggc atagatcaaa ccagtcaacc
     4201 ttcagcaggc ccaaggggca cttaagtgag aaaatgactc ttgacttgaa ggatgaaaat
     4261 gatgcaggca atcttataac atttccaaag gaaagtttgg ctgttggtga ctttaattta
     4321 gggtctaacg tcagcctgga aaaaattaag caggaccaga aactgattga agaaaatgaa
     4381 aaacttaaaa cagagaagga cgctcttctt gaaagttata aggcgttaga attgaaagta
     4441 gagcagattg cccaggagct gcagcaagag aaggcagctg ctgtggattt gactaaccac
     4501 ttagaataca ctctgaagac ctatatagat accagaatga aaaatctggc agctaagatg
     4561 gaaatactga aagaaatgag gcatgtcgac atcagtgtcc gtttcggaaa agacctttca
     4621 gatgctatac aagtgttgga tgaagggtgc tttactactc cagcttcttt gaatggatta
     4681 gagataatat gggcagaata cagtctggct caggagaata ttaaaacttg tgaatatgtg
     4741 agtgaaggga atattttgat tgcccaaaga aatgaaatgc agcagaagct gtacatgtca
     4801 gtagaagatt ttattctgga agttgatgag tcatctctta ataaacgctt aaaaacattg
     4861 caggatttgt cagtctcttt agaagcagtg tatggacaag ccaaagaagg agcaaattct
     4921 gatgaaatac ttaaaaaatt ttatgactgg aagtgtgata aaagagagga gttcaccagt
     4981 gttagaagtg aaacagacgc ttctctgcac cgtcttgtag catggttcca aagaacctta
     5041 aaggtttttg acctatctgt ggaaggatca ctgatttcag aagacgcaat ggataatatt
     5101 gatgaaatcc tagagaagac tgagtcaagt gtctgcaaag agctggagat agctctggtt
     5161 gatcaaggtg atgcagacaa ggagataatt tcaaatacat atagtcaagt actgcaaaag
     5221 attcattcag aggaaaggct cattgccaca gtacaagcta agtacaagga cagtattgag
     5281 tttaaaaagc agcttattga atatttaaat aagagtccca gtgtggatca cttgctatcc
     5341 attaagaaga cattgaaaag cttaaaagct ctactcagat ggaaattggt tgaaaagagt
     5401 aatttggaag agtcagatga tcctgatggc tctcaaattg agaaaataaa agaagaaata
     5461 actcagctgc gcaataatgt ctttcaggaa atttatcatg agagagagga atatgagatg
     5521 ctaactagtt tggcacagaa atggttccct gagctgcctc tgcttcatcc tgaaatagga
     5581 ttactcaaat acatgaactc tggtggtctc cttacaatga gcttggaacg agatcttctt
     5641 gatgctgagc ccatgaagga acttagcagc aagcgtcctt tggtacgttc tgaggttaat
     5701 gggcagataa ttctgttaaa gggctattct gtggatgttg acacagaagc caaggtgatt
     5761 gagagagcag ccacctacca tagagcttgg agagaagctg aaggagactc agggttactg
     5821 ccattgatat tcctgttttt atgtaagtct gatcctatgg cttatctgat ggtcccatac
     5881 taccctaggg caaacctgaa tgctgttcaa gccaacatgc ctttaaattc agaagaaact
     5941 ttaaaggtca tgaaaggtgt tgcccagggt ctgcatacat tgcataaggc tgacataatt
     6001 catggatcac ttcatcagaa caatgtattt gctttaaacc gtgaacaagg aattgttgga
     6061 gattttgact tcaccaaatc tgtgagtcag cgagcctcgg tgaacatgat ggttggtgac
     6121 ttgagtttga tgtcacctga gttgaaaatg ggaaaacctg cttctccagg ttcagactta
     6181 tatgcttatg gctgcctctt attatggctt tctgttcaaa atcaggagtt tgagataaat
     6241 aaagatggaa tccccaaagt ggatcagttt catctggatg ataaagtcaa atccctcctc
     6301 tgtagcttga tatgttatag aagttcaatg actgctgaac aagttttaaa tgctgaatgt
     6361 ttcttgatgc caaaggagca atcagttcca aacccagaaa aagatactga atacacccta
     6421 tataaaaagg aagaagaaat aaagacggag aacttggata aatgtatgga gaagacaaga
     6481 aatggtgaag ccaactttga ttgttagaac ccagctttct tgtacaaagt ggttgatatc
     6541 cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct
     6601 aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagatcc ctcccccccc
     6661 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     6721 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     6781 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     6841 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     6901 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     6961 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     7021 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     7081 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     7141 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     7201 aaaacacgat gataatatgg ccacacatat ggtgagcaag ggcgaggagc tgttcaccgg
     7261 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     7321 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     7381 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     7441 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     7501 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     7561 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     7621 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     7681 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     7741 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     7801 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     7861 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     7921 tctcggcatg gacgagctgt acaagtaaat cgatagatcc taatcaacct ctggattaca
     7981 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     8041 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     8101 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     8161 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     8221 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     8281 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     8341 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     8401 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     8461 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     8521 gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa
     8581 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     8641 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8701 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8761 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8821 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8881 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat
     8941 taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     9001 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     9061 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     9121 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     9181 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     9241 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9301 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9361 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     9421 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9481 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9541 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9601 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9661 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9721 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9781 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9841 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9901 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9961 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    10021 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    10081 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    10141 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    10201 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    10261 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10321 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    10381 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10441 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10501 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10561 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10621 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10681 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10741 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10801 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10861 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    10921 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    10981 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact
    11041 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    11101 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt
    11161 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    11221 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    11281 gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat
    11341 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    11401 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
    11461 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
    11521 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
