LOCUS       sequence-241751-       11113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of GTF2IRD1__ALK fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-GTF2IRD1__ALK
FEATURES             Location/Qualifiers
     source          1..11113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4451..4475)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6173..6193)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6247..6288
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6309..6876
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6326..6878
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6470..6487)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6697..6716
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6890..7489
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6890..6909)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7386..7406
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7503..8091
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7556..7576)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7974..7985)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8166..8399
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8518..8536)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8604..8708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8709..8777,8778..9569)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8927..8946)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9740..10328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10229..10248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10482..10499
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10739..10760
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10775..10780,10781..10798,10799..10805)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10813..10829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10818..10840
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10837..10853
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10837..10853
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10903..10922
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatggcc ttgctgggta agcgctgtga cgtccccacc
     3481 aacggctgcg gacccgaccg ctggaactcc gcgttcaccc gcaaagacga gatcatcacc
     3541 agcctcgtgt ctgccttaga ctccatgtgc tcagcgctgt ccaaactgaa cgccgaggtg
     3601 gcctgtgtcg ccgtgcacga tgagagcgcc tttgtggtgg gcacagagaa ggggagaatg
     3661 ttcctgaatg cccggaagga gctacagtca gacttcctca ggttctgccg agggcccccg
     3721 tggaaggatc cggaggcaga gcaccccaag aaggtgcagc ggggcgaggg tggaggccgt
     3781 agcctccctc ggtcctccct ggaacatggc tcagatgtgt accttctgcg gaagatggta
     3841 gaggaggtgt ttgatgttct ttatagcgag gccctgggaa gggccagtgt ggtgccactg
     3901 ccctatgaga ggctgctcag ggagccaggg ctgctggccg tgcaggggct gcccgagggc
     3961 ctggccttcc gaaggccagc cgagtatgac cccaaggccc tcatggccat cctggaacac
     4021 agccaccgca tccgcttcaa gctcaagagg ccacttgagg atggcgggcg ggactcgaag
     4081 gccctggtgg agctgaacgg tgtctccctg attcccaagg ggtcacggga ctgtggcctg
     4141 catggccagg cccccaaggt gccaccccag gacctgcccc caaccgccac ctcctcctcc
     4201 atggccagct tcctgtacag cacggcgctc cccaaccacg ccatccgaga gctcaagcag
     4261 gaagcacctt cctgccccct tgcccccagc gacctgggcc tgagtcggcc catgccagag
     4321 cccaaggcca ccggtgccca agacttctcc gactgttgtg gacagaagcc cactgggcct
     4381 ggtgggcctc tcatccagaa cgtccatgcc tccaagcgca ttctcttctc catcgtccat
     4441 gacaagtcag tcccagcttt cttgtacaaa gtggttctgt accgccggaa gcaccaggag
     4501 ctgcaagcca tgcagatgga gctgcagagc cctgagtaca agctgagcaa gctccgcacc
     4561 tcgaccatca tgaccgacta caaccccaac tactgctttg ctggcaagac ctcctccatc
     4621 agtgacctga aggaggtgcc gcggaaaaac atcaccctca ttcggggtct gggccatggc
     4681 gcctttgggg aggtgtatga aggccaggtg tccggaatgc ccaacgaccc aagccccctg
     4741 caagtggctg tgaagacgct gcctgaagtg tgctctgaac aggacgaact ggatttcctc
     4801 atggaagccc tgatcatcag caaattcaac caccagaaca ttgttcgctg cattggggtg
     4861 agcctgcaat ccctgccccg gttcatcctg ctggagctca tggcgggggg agacctcaag
     4921 tccttcctcc gagagacccg ccctcgcccg agccagccct cctccctggc catgctggac
     4981 cttctgcacg tggctcggga cattgcctgt ggctgtcagt atttggagga aaaccacttc
     5041 atccaccgag acattgctgc cagaaactgc ctcttgacct gtccaggccc tggaagagtg
     5101 gccaagattg gagacttcgg gatggcccga gacatctaca gggcgagcta ctatagaaag
     5161 ggaggctgtg ccatgctgcc agttaagtgg atgcccccag aggccttcat ggaaggaata
     5221 ttcacttcta aaacagacac atggtccttt ggagtgctgc tatgggaaat cttttctctt
     5281 ggatatatgc cataccccag caaaagcaac caggaagttc tggagtttgt caccagtgga
     5341 ggccggatgg acccacccaa gaactgccct gggcctgtat accggataat gactcagtgc
     5401 tggcaacatc agcctgaaga caggcccaac tttgccatca ttttggagag gattgaatac
     5461 tgcacccagg acccggatgt aatcaacacc gctttgccga tagaatatgg tccacttgtg
     5521 gaagaggaag agaaagtgcc tgtgaggccc aaggaccctg agggggttcc tcctctcctg
     5581 gtctctcaac aggcaaaacg ggaggaggag cgcagcccag ctgccccacc acctctgcct
     5641 accacctcct ctggcaaggc tgcaaagaaa cccacagctg cagagatctc tgttcgagtc
     5701 cctagagggc cggccgtgga agggggacac gtgaatatgg cattctctca gtccaaccct
     5761 ccttcggagt tgcacaaggt ccacggatcc agaaacaagc ccaccagctt gtggaaccca
     5821 acgtacggct cctggtttac agagaaaccc accaaaaaga ataatcctat agcaaagaag
     5881 gagccacacg acaggggtaa cctggggctg gagggaagct gtactgtccc acctaacgtt
     5941 gcaactggga gacttccggg ggcctcactg ctcctagagc cctcttcgct gactgccaat
     6001 atgaaggagg tacctctgtt caggctacgt cacttccctt gtgggaatgt caattacggc
     6061 taccagcaac agggcttgcc cttagaagcc gctactgccc ctggagctgg tcattacgag
     6121 gataccattc tgaaaagcaa gaatagcatg aaccagcctg ggccctgaca cccaactttt
     6181 ctatacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg
     6241 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag
     6301 taatgagatc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     6361 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     6421 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     6481 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     6541 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     6601 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     6661 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     6721 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     6781 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     6841 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacacata tgaccgagta
     6901 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     6961 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     7021 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     7081 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     7141 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     7201 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     7261 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     7321 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     7381 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     7441 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaaa tcgatagatc
     7501 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7561 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7621 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7681 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7741 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7801 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7861 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7921 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7981 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     8041 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     8101 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8161 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     8221 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8281 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8341 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8401 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8461 gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     8521 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8581 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8641 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8701 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8761 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8821 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8881 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8941 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9001 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9061 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9121 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9181 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9241 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9301 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9361 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9421 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9481 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9541 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9601 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9661 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9721 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9781 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9841 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9901 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9961 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10021 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10081 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10141 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10201 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10261 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10321 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10381 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10441 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10501 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10561 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
    10621 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    10681 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    10741 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10801 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
    10861 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10921 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    10981 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
    11041 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
    11101 caaacttagt agt
//
