LOCUS       sequence-241753-       10546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of GSK3A__ZNF564 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-GSK3A__ZNF564
FEATURES             Location/Qualifiers
     source          1..10546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3916..3940)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5606..5626)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5680..5721
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5742..6309
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5759..6311
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5903..5920)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6130..6149
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6323..6922
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6323..6342)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6819..6839
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6936..7524
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6989..7009)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7407..7418)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7599..7832
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7951..7969)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8037..8141
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8142..8210,8211..9002)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8360..8379)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9173..9761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9662..9681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9915..9932
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10172..10193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10208..10213,10214..10231,10232..10238)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10246..10262
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10251..10273
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10270..10286
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10270..10286
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10336..10355
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatgagc ggcggcgggc cttcgggagg cggccctggg
     3481 ggctcgggca gggcgcggac tagctcgttc gcggagcccg gcggcggagg cggaggaggc
     3541 ggcggcggcc ccggaggctc ggcctccggc ccaggcggca ccggcggcgg aaaggcatct
     3601 gtcggggcca tgggtggggg cgtcggggcc tcgagctccg ggggtggacc cggcggcagc
     3661 ggcggaggag gcagcggagg ccccggcgca ggcactagct tcccgccgcc cggggtgaag
     3721 ctgggccgtg acagcgggaa ggtgaccaca gtcgtagcca ctctaggcca aggcccagag
     3781 cgctcccaag aagtggctta cacggacatc aaagtgattg gcaatggctc atttggggtc
     3841 gtgtaccagg cacggctggc agagaccagg gaactagtcg ccatcaagaa ggttctccag
     3901 gacaagaggt tcaagtccca gctttcttgt acaaagtggt tcgactcagt ggcctctgag
     3961 gatgtggctg tgaacttcac acttgaggag tgggctttgc tggatccttc ccagaagaaa
     4021 ctctacagag atgtgatgcg ggaaaccttt agaaacctgg cctgtgtagg aaaaaaatgg
     4081 gaagaccaga gcattgaaga ttggtacaaa aatcagggga gaattttaag aaatcatatg
     4141 gaagagggac tcagtgaaag taaagaatat gatcaatgtg gagaagcctt cagtcagatt
     4201 ctcaatctta atctgaacaa gaaaattcct actatagtaa gaccatgtga atgtagtttg
     4261 tgtgggaaag tcttcatgca tcattcatcc cttagtaggc acatcagatc tcaccttgga
     4321 cacaaaccat atgactatca ggaatatgga gagaaaccat ataaatgtaa gcagtgtggg
     4381 aaagccttca gttcttgtca atcctttcga agacatgaaa gaactcacac tggtgagaaa
     4441 ccctatgcat gtccggaatg tgggaaagcc ttcatttctc tcccaagtgt tcgaagacac
     4501 atgattaagc acactggaga tggaccatat aaatgtcagg aatgtgggaa agcctttgat
     4561 cgcccaagtt tatttcagat acatgaaaga actcacactg gagagaaacc ctatgaatgt
     4621 caggaatgtg caaaagcttt catttctctt ccaagttttc aaagacacat gattaggcac
     4681 actggagatg gaccttataa atgtcaggaa tgtggaaaag cctttgaccg ccccagttta
     4741 tttcgaatac atgaaagaac tcatactgga gagaaacccc atgaatgtaa acagtgtggg
     4801 aaggccttca tttctttcac aaattttcaa agtcatatga ttaggcacac tggggatgga
     4861 ccttataaat gtaaagtatg tgggagagcc tttatttttc ccagttatgt tcgaaagcat
     4921 gaaagaactc atactgggga gaaaccctat gaatgtaata aatgtggtaa aaccttcagt
     4981 tcttccagta atgttcgaac acatgaaagg actcacactg gagagaagcc ctatgaatgt
     5041 aaggaatgcg ggaaagcctt catttctctc ccaagtgtcc gaagacacat gataaagcac
     5101 actggagatg gaccttataa atgtcaggta tgtggtagag cctttgactg tcccagttca
     5161 tttcaaatac acgaaagaac tcacactgga gagaaaccct atgaatgtca ggtatgtggg
     5221 aaagccttca tttctcttaa aaggattaga aaacatatga tactgcacac tggagatgga
     5281 ccttataaat gtcaggtatg tggtaaagcc tttgactgtc ctagttctgt ccgaacacat
     5341 gaaagaactc atactggaga aaaaccctat gaatgtaaag aatgtgggaa agcattcaat
     5401 tatgccagtt ccattagaat acatgaaaga actcataccg gagaaaaacc ctatgaatgt
     5461 aagcaatgtg gaaaaacgtt cagttattcc agttcctttc aaagacatga aagagctcat
     5521 aatggagata aaccttacgt aaagaatgtg ggaaagcttt catttatcac acaaccttcg
     5581 aatacctgtg aaaatgaata gcacccaact tttctataca aagtggttga tatccagcac
     5641 agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     5701 ctcctcggtc tcgattctac gcgtaccggt tagtaatgag atccctcccc cccccctaac
     5761 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     5821 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     5881 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     5941 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     6001 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     6061 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     6121 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     6181 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     6241 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     6301 cgatgataat atggccacac atatgaccga gtacaagccc acggtgcgcc tcgccacccg
     6361 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     6421 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     6481 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     6541 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     6601 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     6661 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     6721 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     6781 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     6841 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     6901 gacccgcaag cccggtgcct aaatcgatag atcctaatca acctctggat tacaaaattt
     6961 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7021 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7081 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     7141 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7201 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7261 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7321 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     7381 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7441 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7501 tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc
     7561 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7621 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7681 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7741 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7801 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7861 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga
     7921 aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     7981 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     8041 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8101 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8161 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8221 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8281 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8341 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     8401 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8461 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8521 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8581 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8641 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8701 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8761 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8821 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8881 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8941 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9001 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9061 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9121 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9181 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9241 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9301 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9361 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9421 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9481 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9541 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9601 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9661 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9721 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     9781 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     9841 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     9901 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     9961 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt
    10021 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    10081 aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg
    10141 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    10201 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    10261 aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt
    10321 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    10381 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    10441 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
    10501 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt
//
