LOCUS       sequence-241761-        6038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter containing 13 copies of the p53-binding 
            consensus sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, 
            Lin D, Mercer WE, Kinzler KW, Vogelstein B
  TITLE     WAF1, a potential mediator of p53 tumor suppression.
  JOURNAL   Cell. 1993 Nov 19. 75(4):817-25.
  PUBMED    8242752
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1993 
            Nov 19. 75(4):817-25."
COMMENT     Sequence Label: PG13-luc (wt p53 binding sites)
FEATURES             Location/Qualifiers
     source          1..6038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(421..437)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             491..2143
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(557..575)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2274..2339
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2469..2489
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2914..3048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2951..2970)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3005..3024
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3045..3061)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3098..3116)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3137..3153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3137..3153)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3150..3172)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3161..3177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3185..3191,3192..3209,3210..3215))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3230..3251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3368..3385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3539..4127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3619..3638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4298..5089,5090..5158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4921..4940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5159..5263)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5289..5744)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5426..5447)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5638..5657
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5871..5893
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5885..5902
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5886..5902
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5909..5928
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5909..5927
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     5952..5968
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5953..5969
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccaggcaagt ccaggcaggc caggcaagtc caggcaggcc aggcaagtcc aggcaggcca
       61 ggcaagtcca ggcaggccag gcaagtccag gcaggccagg caagtccagg caggccaggc
      121 aagtccaggc agggccaggc aagtccaggc aggccaggca agtccaggca ggccaggcaa
      181 gtccaggcag gatcgaattc gatctgtttt ttttagtatt aagcagaggc cgggggcccc
      241 tggcctccgc ttactctgga gaaaaagaag agaggcattc cagaggcaac ttgtcaaaac
      301 aggactggcg ccttggaggc gctgtggggc cacccaaatt gatataatta agccccaacc
      361 gcctcttccc gcctcatttc agcctcacca ccctcgacaa gcttatcgat accgtcgggg
      421 gatccactag ttctagagcg gccgccaccg cggtggagct cgagcttggc attccggtac
      481 tgttggtaaa atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct
      541 agaggatgga accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc
      601 tggaacaatt gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt
      661 cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag
      721 aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt
      781 tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag
      841 tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt
      901 gaacgtgcaa aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga
      961 ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa
     1021 tgaatacgat tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa
     1081 ttcctctgga tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt
     1141 cagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat
     1201 tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat
     1261 atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct
     1321 tcaggattac aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa
     1381 aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc
     1441 acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg
     1501 acaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga
     1561 taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga
     1621 taccgggaaa acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat
     1681 tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg
     1741 gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg
     1801 cttgaagtct ttaattaaat acaaaggata tcaggtggcc cccgctgaat tggaatcgat
     1861 attgttacaa caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc
     1921 cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga
     1981 gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt
     2041 gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga
     2101 gatcctcata aaggccaaga agggcggaaa gtccaaattg taaaatgtaa ctgtattcag
     2161 cgatgacgaa attcttagct attgtaatac tctagaggat ctttgtgaag gaaccttact
     2221 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
     2281 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
     2341 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
     2401 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
     2461 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
     2521 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
     2581 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct
     2641 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc
     2701 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt
     2761 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc
     2821 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2881 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2941 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3001 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcca ctagttctag
     3061 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaatttcga
     3121 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3181 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3241 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     3301 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     3361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3961 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4201 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4261 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4321 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     4381 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     4441 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4501 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4561 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4621 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4681 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4741 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4801 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4861 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4921 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4981 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5041 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5101 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5161 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5221 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     5281 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     5341 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     5401 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     5461 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     5521 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     5581 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     5641 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     5701 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     5761 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     5821 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     5881 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattgggtac
     5941 cgggcccccc ctcgaggtcg acggtatcga taagcttgat gccaggcaag tccaggcagg
     6001 ccaggcaagt ccaggcaggc caggcaagtc caggcagg
//
