LOCUS       sequence-241770-       10741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of DUSP22__PTK7 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-DUSP22__PTK7
FEATURES             Location/Qualifiers
     source          1..10741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3953..3977)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5801..5821)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5875..5916
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5937..6504
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5954..6506
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6098..6115)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6325..6344
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6518..7117
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6518..6537)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7014..7034
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7131..7719
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7184..7204)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7602..7613)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7794..8027
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8146..8164)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8232..8336
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8337..8405,8406..9197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8555..8574)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9368..9956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9857..9876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10110..10127
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10367..10388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10403..10408,10409..10426,10427..10433)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10441..10457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10446..10468
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10465..10481
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10465..10481
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10531..10550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatgggg aatgggatga acaagatcct gcccggcctg
     3481 tacatcggca acttcaaaga tgccagagac gcggaacaat tgagcaagaa caaggtgaca
     3541 catattctgt ctgtccacga tagtgccagg cctatgttgg agggagttaa atacctgtgc
     3601 atcccagcag cggattcacc atctcaaaac ctgacaagac atttcaaaga aagtattaaa
     3661 ttcattcacg agtgccggct ccgcggtgag agctgccttg tacactgcct ggccggggtc
     3721 tccaggagcg tgacactggt gatcgcatac atcatgaccg tcactgactt tggctgggag
     3781 gatgccctgc acaccgtgcg tgctgggaga tcctgtgcca accccaacgt gggcttccag
     3841 agacagctcc aggagtttga gaagcatgag gtccatcagt atcggcagtg gctgaaggaa
     3901 gaatatggag agagcccttt gcaggatgca gaagaagcca aaaacattct ggtcccagct
     3961 ttcttgtaca aagtggttcc tgtgccctcc tggctgaaga agccccaaga cagccagctg
     4021 gaggagggca aacccggcta cttggattgc ctgacccagg ccacaccaaa acctacagtt
     4081 gtctggtaca gaaaccagat gctcatctca gaggactcac ggttcgaggt cttcaagaat
     4141 gggaccttgc gcatcaacag cgtggaggtg tatgatggga catggtaccg ttgtatgagc
     4201 agcaccccag ccggcagcat cgaggcgcaa gcccgtgtcc aagtgctgga aaagctcaag
     4261 ttcacaccac caccccagcc acagcagtgc atggagtttg acaaggaggc cacggtgccc
     4321 tgttcagcca caggccgaga gaagcccact attaagtggg aacgggcaga tgggagcagc
     4381 ctcccagagt gggtgacaga caacgctggg accctgcatt ttgcccgggt gactcgagat
     4441 gacgctggca actacacttg cattgcctcc aacgggccgc agggccagat tcgtgcccat
     4501 gtccagctca ctgtggcagt ttttatcacc ttcaaagtgg aaccagagcg tacgactgtg
     4561 taccagggcc acacagccct actgcagtgc gaggcccagg gggaccccaa gccgctgatt
     4621 cagtggaaag acaagcctgt gccggaggag tcggagggcc ctggcagccc tcccccctac
     4681 aagatgatcc agaccattgg gttgtcggtg ggtgccgctg tggcctacat cattgccgtg
     4741 ctgggcctca tgttctactg caagaagcgc tgcaaagcca agcggctgca gaagcagccc
     4801 gagggcgagg agccagagat ggaatgcctc aacggtgggc ctttgcagaa cgggcagccc
     4861 tcagcagaga tccaagaaga agtggccttg accagcttgg gctccggccc cgcggccacc
     4921 aacaaacgcc acagcacaag tgataagatg cacttcccac ggtctagcct gcagcccatc
     4981 accacgctgg ggaagagtga gtttggggag gtgttcctgg caaaggctca gggcttggag
     5041 gagggagtgg cagagaccct ggtacttgtg aagagcctgc agagcaagga tgagcagcag
     5101 cagctggact tccggaggga gttggagatg tttgggaagc tgaaccacgc caacgtggtg
     5161 cggctcctgg ggctgtgccg ggaggctgag ccccactaca tggtgctgga atatgtggat
     5221 ctgggagacc tcaagcagtt cctgaggatt tccaagagca aggatgaaaa attgaagtca
     5281 cagcccctca gcaccaagca gaaggtggcc ctatgcaccc aggtagccct gggcatggag
     5341 cacctgtcca acaaccgctt tgtgcataag gacttggctg cgcgtaactg cctggtcagt
     5401 gcccagagac aagtgaaggt gtctgccctg ggcctcagca aggatgtgta caacagtgag
     5461 tactaccact tccgccaggc ctgggtgccg ctgcgctgga tgtcccccga ggccatcctg
     5521 gagggtgact tctctaccaa gtctgatgtc tgggccttcg gtgtgctgat gtgggaagtg
     5581 tttacacatg gagagatgcc ccatggtggg caggcagatg atgaagtact ggcagatttg
     5641 caggctggga aggctagact tcctcagccc gagggctgcc cttccaaact ctatcggctg
     5701 atgcagcgct gctgggccct cagccccaag gaccggccct ccttcagtga gattgccagc
     5761 gccctgggag acagcaccgt ggacagcaag ccgtgacacc caacttttct atacaaagtg
     5821 gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag
     5881 cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc
     5941 tccccccccc ntaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     6001 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     6061 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     6121 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     6181 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     6241 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     6301 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     6361 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     6421 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     6481 ttcctttgaa aaacacgatg ataatatggc cacacatatg accgagtaca agcccacggt
     6541 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     6601 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     6661 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     6721 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     6781 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     6841 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     6901 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     6961 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     7021 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     7081 gcgcacctgg tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct aatcaacctc
     7141 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     7201 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     7261 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7321 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7381 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7441 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7501 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7561 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7621 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7681 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat
     7741 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7801 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     7861 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7921 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7981 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8041 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     8101 cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc
     8161 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     8221 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8281 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8341 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8401 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8461 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8521 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     8581 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8641 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8701 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8761 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8821 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8881 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8941 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9001 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     9061 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9121 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9181 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9241 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9301 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9421 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9481 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9541 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9601 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9661 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9721 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9781 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9841 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9901 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9961 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10021 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    10081 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    10141 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca
    10201 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    10261 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc
    10321 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    10381 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    10441 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa
    10501 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10561 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    10621 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    10681 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    10741 t
//
