LOCUS       sequence-241773-       12184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of CDC42BPB__SYNJ2BP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-CDC42BPB__SYNJ2BP
FEATURES             Location/Qualifiers
     source          1..12184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6976..7000)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7244..7264)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7318..7359
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7380..7947
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7397..7949
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7541..7558)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7768..7787
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7961..8560
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7961..7980)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8457..8477
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8574..9162
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8627..8647)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9045..9056)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9237..9470
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9589..9607)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9675..9779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9780..9848,9849..10640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9998..10017)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10811..11399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11300..11319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11553..11570
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11810..11831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11846..11851,11852..11869,11870..11876)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11884..11900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11889..11911
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11908..11924
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11908..11924
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11974..11993
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatgtcg gccaaggtgc ggctcaagaa gctggagcag
     3481 ctgctcctgg acgggccctg gcgcaacgag agcgccctga gcgtggaaac gctgctcgac
     3541 gtgctcgtct gcctgtacac cgagtgcagc cactcggccc tgcgccgcga caagtacgtg
     3601 gccgagttcc tcgagtgggc taaaccattt acacagctgg tgaaagaaat gcagcttcat
     3661 cgagaagact ttgaaataat taaagtaatt ggaagaggtg cttttggtga ggttgctgtt
     3721 gtcaaaatga agaatactga acgaatttat gcaatgaaaa tcctcaacaa gtgggagatg
     3781 ctgaaaagag cagagaccgc gtgcttccga gaggagcgcg atgtgctggt gaacggcgac
     3841 tgccagtgga tcaccgcgct gcactacgcc tttcaggacg agaaccacct gtacttagtc
     3901 atggattact atgtgggtgg tgatttactg accctgctca gcaaatttga agacaagctt
     3961 ccggaagata tggcgaggtt ctacattggt gaaatggtgc tggccattga ctccatccat
     4021 cagcttcatt acgtgcacag agacattaaa cctgacaatg tccttttgga cgtgaatggt
     4081 catatccgcc tggctgactt tggatcatgt ttgaagatga atgatgatgg cactgtgcag
     4141 tcctccgtgg ccgtgggcac acctgactac atctcgccgg agatcctgca ggcgatggag
     4201 gacggcatgg gcaaatacgg gcctgagtgt gactggtggt ctctgggtgt ctgcatgtat
     4261 gagatgctct atggagaaac gccgttttat gcggagtcac tcgtggagac ctatgggaag
     4321 atcatgaacc atgaagagcg attccagttc ccatcccatg tcacggatgt atctgaagaa
     4381 gcgaaggacc tcatccagag actgatctgc agtagagaac gccggctggg gcagaatgga
     4441 atagaggatt tcaaaaagca tgcgtttttt gaaggtctaa attgggaaaa tatacgaaac
     4501 ctagaagcac cttatattcc tgatgtgagc agtccctctg acacatccaa cttcgacgtg
     4561 gatgacgacg tgctgagaaa cacggaaata ttacctcctg gttctcacac aggcttttct
     4621 ggattacatt tgccattcat tggttttaca ttcacaacgg aaagctgttt ttctgatcga
     4681 ggctctctga agagcataat gcagtccaac acattaacca aagatgagga tgtgcagcgg
     4741 gacctggagc acagcctgca gatggaagct tacgagagga ggattcggag gctggaacag
     4801 gagaagctgg agctgagcag gaagctgcaa gagtccaccc agaccgtgca gtccctccac
     4861 ggctcatctc gggccctcag caattcaaac cgagataaag aaatcaaaaa gctaaatgaa
     4921 gaaatcgaac gcttgaagaa taaaatagca gattcaaaca ggctcgagcg acagcttgag
     4981 gacacagtgg cgcttcgcca agagcgtgag gactccacgc agcggctgcg ggggctggag
     5041 aagcagcacc gcgtggtccg gcaggagaag gaggagctgc acaagcaact ggttgaagcc
     5101 tcagagcggt tgaaatccca ggccaaggaa ctcaaagatg cccatcagca gcgaaagctg
     5161 gccctgcagg agttctcgga gctgaacgag cgcatggcag agctccgtgc ccagaagcag
     5221 aaggtgtccc ggcagctgcg agacaaggag gaggagatgg aggtggccac gcagaaggtg
     5281 gacgccatgc ggcaggaaat gcggagagct gagaagctca ggaaagagct ggaagctcag
     5341 cttgatgatg ctgttgctga ggcctccaag gagcgcaagc ttcgtgagca cagcgagaac
     5401 ttctgcaagc aaatggaaag cgagctggag gccctcaagg tgaagcaagg aggccgggga
     5461 gcgggtgcca ccttagagca ccagcaagag atttccaaaa tcaaatccga gctggagaag
     5521 aaagtcttat tttatgaaga ggaattggtc agacgtgagg cctcccatgt gctagaagtg
     5581 aaaaatgtga agaaggaggt gcatgattca gaaagccacc agctggccct gcagaaagaa
     5641 atcttgatgt taaaagataa gttagaaaag tcaaagcgag aacggcataa cgagatggag
     5701 gaggcagtag gtacaataaa agataaatac gaacgagaaa gagcgatgct gtttgatgaa
     5761 aacaagaagc taactgctga aaatgaaaag ctctgttcct ttgtggataa actcacagct
     5821 caaaatagac agctggagga tgagctgcag gatctggcag ccaagaagga gtcagtggcc
     5881 cactgggaag ctcagattgc ggaaatcatt cagtgggtca gtgacgagaa agatgcccgg
     5941 ggttaccttc aagctcttgc ttccaagatg accgaagagc tcgaggcttt gaggagttct
     6001 agtctggggt caagaacact ggacccgctg tggaaggtgc gccgcagcca gaagctggac
     6061 atgtccgcgc ggctggagct gcagtcggcc ctggaggcgg agatccgggc caagcagctt
     6121 gtccaggagg agctcaggaa ggtcaaggac gccaacctca ccttggaaag caaactaaag
     6181 gattccgaag ccaaaaacag agaattatta gaagaaatgg aaattttgaa gaaaaagatg
     6241 gaagaaaaat tcagagcaga tactgggctc aaacttccag attttcagga ttccattttt
     6301 gagtatttca acactgctcc tcttgcacat gacctgacat ttagaaccag ctcagctagt
     6361 gagcaagaaa cacaagctcc gaagccagaa gcgtccccgt cgatgtctgt ggctgcatca
     6421 gagcagcagg aggacatggc tcggcccccg cagaggccat ccgctgtgcc gttgcccacc
     6481 acgcaggccc tggctctggc tggaccgaag ccaaaagctc accagttcag catcaagtcc
     6541 ttctccagcc ctactcagtg cagccactgc acctccctga tggttgggct gatccggcag
     6601 ggctacgcct gcgaggtgtg ttcctttgct tgccacgtgt cctgcaaaga cggtgccccc
     6661 caggtgtgcc caatacctcc cgagcagtcc aagaggcctc tgggcgtgga cgtgcagcga
     6721 ggcatcggaa cagcctacaa aggccatgtc aaggtcccaa agcccacggg ggtgaagaag
     6781 ggatggcagc gcgcatatgc agtcgtctgt gactgcaagc tcttcctgta tgatctgcct
     6841 gaaggaaaat ccacccagcc tggtgtcatt gcgagccaag tcttggatct cagagatgac
     6901 gagttttccg tgagctcagt cctggcctca gatgtcattc atgctacacg ccgagatatt
     6961 ccatgtatat tcaggtccca gctttcttgt acaaagtggt tcgtaaatgg ccaagaccta
     7021 aagaacctgc tgcaccagga tgctgtagac ctctttcgta atgcaggcta tgctgtgtct
     7081 ctgagagtgc agcacaggtt acaggtgcag aatggaccta taggacatcg aggtgaaggg
     7141 gacccaagtg gtattcccat atttatggtg ctggtgccag tgtttgccct caccatggta
     7201 gcagcctggg ctttcatgag ataccggcaa caactttgac acccaacttt tctatacaaa
     7261 gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
     7321 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagat
     7381 ccctcccccc nccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     7441 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     7501 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     7561 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     7621 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     7681 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     7741 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     7801 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     7861 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     7921 gttttccttt gaaaaacacg atgataatat ggccacacat atgaccgagt acaagcccac
     7981 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     8041 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     8101 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     8161 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     8221 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     8281 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     8341 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     8401 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     8461 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     8521 accgcgcacc tggtgcatga cccgcaagcc cggtgcctaa atcgatagat cctaatcaac
     8581 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     8641 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     8701 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     8761 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     8821 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     8881 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     8941 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     9001 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     9061 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     9121 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
     9181 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     9241 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     9301 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9361 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9421 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     9481 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     9541 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
     9601 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     9661 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9721 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9781 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9841 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9901 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9961 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10021 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10081 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10141 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10201 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10261 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10321 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10381 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10441 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10501 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10561 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10621 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10681 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10741 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10801 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10861 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10921 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10981 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11041 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11101 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11161 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11221 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11281 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11341 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11401 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    11461 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11521 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    11581 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
    11641 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    11701 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
    11761 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    11821 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    11881 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
    11941 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    12001 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    12061 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    12121 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    12181 tagt
//
