LOCUS       sequence-241777-       17569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of AKAP13__RET fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17569)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 17569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-AKAP13__RET
FEATURES             Location/Qualifiers
     source          1..17569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11515..11539)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12629..12649)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             12703..12744
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    12765..13332
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    12782..13334
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12926..12943)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     13153..13172
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             13346..13945
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(13346..13365)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13842..13862
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    13959..14547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(14012..14032)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14430..14441)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             14622..14855
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(14974..14992)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        15060..15164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15165..15233,15234..16025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15383..15402)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16196..16784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16685..16704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16938..16955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    17195..17216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(17231..17236,17237..17254,17255..17261)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17269..17285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17274..17296
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17293..17309
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17293..17309
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     17359..17378
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatgaaa cttaatccac agcaagctcc cttatatggt
     3481 gattgtgttg ttacagtgct gcttgctgaa gaggacaaag ctgaagatga tgtagtgttt
     3541 tacttggtat ttttgggttc caccctccgt cactgtacaa gtactcggaa ggtcagttct
     3601 gatacattgg agaccattgc tcctggtcat gattgttgtg aaacagtgaa ggtgcagctc
     3661 tgtgcttcca aagagggcct tcccgtgttt gtggtggctg aagaagactt tcatttcgtc
     3721 caggatgaag cgtatgatgc agctcaattc ctagcaacca gtgctggaaa tcagcaggct
     3781 ttgaacttta cccgttttct tgaccagtca ggacccccat ctggggatgt gaattccctt
     3841 gataagaagt tggtgctggc attcaggcac ctgaagctgc ccacggagtg gaatgtattg
     3901 gggacagatc agagtttgca tgatgctggc ccgcgagaga cattgatgca ttttgctgtg
     3961 cggctgggac tgctgaggtt gacgtggttc ctgttgcaga agccaggtgg ccgcggagct
     4021 ctcagtatcc acaaccagga aggggcgacg cctgtgagct tggccttgga gcgaggctat
     4081 cacaagctgc accagcttct aaccgaggag aatgctggag aaccagactc ctggagcagt
     4141 ttatcctatg aaataccgta tggagactgt tctgtgaggc atcatcgaga gttggacatc
     4201 tatacattaa cctctgagtc tgattcacat catgaacacc catttcctgg agacggttgc
     4261 actggaccaa tttttaaact tatgaacatc caacagcaac taatgaaaac aaacctcaag
     4321 cagatggaca gtcttatgcc cttaatgatg acagcacagg atccttccag tgccccagag
     4381 acagatggcc agtttcttcc ctgtgcaccg gagcccacgg accctcagcg actttcttct
     4441 tctgaagaga ctgagagcac tcagtgctgc ccagggagcc ctgttgcaca gactgaaagt
     4501 ccctgtgatt tgtcaagcat agttgaggag gagaatacag accgttcctg taggaagaaa
     4561 aataaaggcg tggaaagaaa aggggaagag gtggagccag cacctattgt ggactctgga
     4621 actgtatctg atcaagacag ctgccttcag agcttgcctg attgtggagt aaagggcacg
     4681 gaaggccttt cgtcctgtgg aaacagaaat gaagaaactg gaacaaaatc ttctggaatg
     4741 cccacagacc aggagtccct gagcagtgga gatgctgtgc ttcagagaga cttggtcatg
     4801 gagccaggca cagcccagta ttcctctgga ggtgaactgg gaggcatttc aacaacaaat
     4861 gtcagtaccc cagacactgc aggggaaatg gaacatgggc tcatgaaccc agatgccact
     4921 gtttggaaga atgtgcttca gggaggggaa agtacaaagg aaagatttga gaactctaat
     4981 attggcacag ctggagcctc tgacgtgcac gtcacaagta agcctgtgga taaaatcagt
     5041 gttccaaact gtgcccctgc tgccagttcc ctggatggta acaaacctgc tgagtcttca
     5101 cttgcattta gtaatgaaga aacctccact gaaaaaacag cagaaacgga aacttcacga
     5161 agtcgtgagg agagtgctga tgctccagta gatcagaatt ctgtggtgat tccagctgct
     5221 gcaaaagaca agatttcaga tggattagaa ccttatactc tcttagcagc aggcataggt
     5281 gaggcaatgt caccctcaga tttagccctt cttgggctgg aagaagatgt aatgccacac
     5341 cagaactcag aaacaaattc atctcatgct caaagccaaa agggcaaatc ctcacccatt
     5401 tgttctacaa ctggagacga taaactttgt gcagactctg catgtcaaca gaacacagtg
     5461 acttctagtg gcgatttggt tgcaaaactg tgtgataaca tagttagcga gtccgaaagc
     5521 accacagcaa ggcaacccag ctcacaagat ccacccgatg cctcccactg tgaagaccca
     5581 caggctcata cagtcacctc tgaccctgta agggataccc aggaacgtgc ggatttttgt
     5641 cctttcaaag tggtggataa caaaggccaa cgaaaagatg tgaaactaga taaaccttta
     5701 acaaatatgc ttgaggtggt ttcacatcca catccagttg tccctaaaat ggagaaagaa
     5761 ctggtgccag accaggcagt aatatcagac agtactttct ctctggcaaa cagtccaggc
     5821 agtgaatcag taaccaagga tgacgcactt tcttttgtcc cctcccagaa agaaaaggga
     5881 acagcaactc ctgaactaca tacagctaca gattatagag atggcccaga tggaaattcg
     5941 aatgagcctg atacgcggcc actagaagac agggcagtag gcctgtccac atcctccact
     6001 gctgcagagc ttcagcacgg gatggggaat accagtctca caggacttgg tggagagcat
     6061 gagggtcccg cccctccagc aatcccagaa gctctgaata tcaaggggaa cactgactct
     6121 tccctgcaaa gtgtgggtaa ggccactttg gctttagatt cagttttgac tgaagaagga
     6181 aaacttctgg tggtttcaga aagctctgca gctcaggaac aagataagga taaagcggtg
     6241 acctgttcct ctattaagga aaatgctctc tcttcaggaa ctttgcagga agagcagaga
     6301 acaccacctc ctggacaaga tactcaacaa tttcatgaaa aatcaatctc agctgactgt
     6361 gccaaggaca aagcacttca gctaagtaat tcaccgggtg catcctctgc ctttcttaag
     6421 gcagaaactg aacataacaa ggaagtggcc ccacaagtct cactgctgac tcaaggtggg
     6481 gctgcccaga gcctggtgcc accaggagca agtctggcca cagagtcaag gcaggaagcc
     6541 ttgggggcag agcacaacag ctccgctctg ttgccatgtc tgttgccaga tgggtctgat
     6601 gggtccgatg ctcttaactg cagtcagcct tctcctctgg atgttggagt gaagaacact
     6661 caatcccagg gaaaaactag tgcctgtgag gtgagtggag atgtgacggt ggatgttaca
     6721 ggggttaatg ctctacaagg tatggctgag cccagaagag agaatatatc acacaacacc
     6781 caagacatcc tgattccaaa cgtcttgttg agccaagaga agaatgccgt tctaggtttg
     6841 ccagtggctc tacaggacaa agctgtgact gacccacagg gagttggaac cccagagatg
     6901 atacctcttg attgggagaa agggaagctg gagggagcag accacagctg taccatgggt
     6961 gacgctgagg aagcccaaat agacgatgaa gcacatcctg tcctactgca gcctgttgcc
     7021 aaggagctcc ccacagacat ggagctctca gcccatgatg atggggcccc agctggtgtg
     7081 agggaagtca tgcgagcccc gccttcaggc agggaaagga gcactccctc tctaccttgc
     7141 atggtctctg cccaggacgc acctctgcct aagggggcag acttgataga ggaggctgcc
     7201 agccgtatag tggatgctgt catcgaacaa gtcaaggccg ctggagcact gcttactgag
     7261 ggggaggcct gtcacatgtc actgtccagc cctgagttgg gtcctctcac taaaggacta
     7321 gagagtgctt ttacagaaaa agtgagtact ttcccacctg gggagagcct accaatgggc
     7381 agtactcctg aggaagccac ggggagcctt gcaggatgtt ttgctggaag ggaggagcca
     7441 gagaagatca ttttacctgt ccaggggcct gagccagcag cagaaatgcc agacgtgaaa
     7501 gctgaagatg aagtggattt tagagcaagt tcaatttctg aagaagtggc tgtagggagc
     7561 atagctgcta cactgaagat gaagcaaggc ccaatgaccc aggcgataaa ccgagaaaac
     7621 tggtgtacaa tagagccatg ccctgatgca gcatctcttc tggcttccaa gcagagccca
     7681 gaatgtgaga acttcctgga tgttggactg ggcagagagt gtacctcaaa acaaggtgta
     7741 cttaaaagag aatctgggag tgattctgac ctctttcact cacccagtga tgacatggac
     7801 agcatcatct tcccaaagcc agaggaagag catttggcct gtgatatcac cggatccagt
     7861 tcatccaccg atgacacggc ttcactggac cgacattctt ctcatggcag tgatgtgtct
     7921 ctctcccaga ttttaaagcc aaacaggtca agagatcggc aaagccttga tggattctac
     7981 agccatggga tgggagctga gggtcgagaa agtgagagtg agcctgctga cccaggcgac
     8041 gtggaggagg aggagatgga cagtatcact gaagtgcctg caaactgctc tgtcctaagg
     8101 agctccatgc gctctctttc tcccttccgg aggcacagct gggggcctgg gaaaaatgca
     8161 gccagcgatg cagaaatgaa ccaccggagt atgagctggt gcccctctgg tgtgcagtac
     8221 tctgctggcc tgagtgctga ctttaattac agaagtttca gtctagaagg cttgacagga
     8281 ggagctggtg tcggaaacaa gccatcctca tctctagaag taagctctgc aaatgccgaa
     8341 gagctcagac acccattcag tggtgaggaa cgggttgact ctttggtgtc actttcagaa
     8401 gaggatctgg agtcagacca gagagaacat aggatgtttg atcagcagat atgtcacaga
     8461 tctaagcagc agggatttaa ttactgtaca tcagccattt cctctccatt gacaaaatcc
     8521 atctcattaa tgacaatcag ccatcctgga ttggacaatt cacggccctt ccacagtacc
     8581 ttccacaata ccagtgctaa tctgactgag agtataacag aagagaacta taatttcctg
     8641 ccacatagcc cctccaagaa agattctgaa tggaagagtg gaacaaaagt cagtcgtaca
     8701 ttcagctaca tcaagaataa aatgtctagc agcaagaaga gcaaagaaaa ggaaaaagaa
     8761 aaagataaga ttaaggagaa ggagaaagat tctaaagaca aggagaaaga taagaagact
     8821 gtcaacgggc acactttcag ttccattcct gttgtgggtc ccatcagctg tagccagtgt
     8881 atgaagccct tcaccaacaa agatgcctat acttgtgcaa attgcagtgc ttttgtccac
     8941 aaaggctgcc gagaaagtct agcctcctgt gcaaaggtca aaatgaagca gcccaaaggg
     9001 agccttcagg cacatgacac atcatcactg cccacggtca ttatgagaaa caagccctca
     9061 cagcccaagg agcgtcctcg gtccgcagtc ctcctggtgg atgaaaccgc taccacccca
     9121 atatttgcca atagacgatc ccagcagagt gtctcgctct ccaaaagtgt ctccatacag
     9181 aacattactg gagttggcaa tgatgagaac atgtcaaaca cctggaaatt cctgtctcat
     9241 tcaacagact cactaaataa aatcagcaag gtcaatgagt caacagaatc acttactgat
     9301 gagggagtag gtacagacat gaatgaagga caactactgg gagactttga gattgagtcc
     9361 aaacagctgg aagcagagtc ttggagtcgg ataatagaca gcaagtttct aaaacagcaa
     9421 aagaaagatg tggtcaaacg gcaagaagta atatatgagt tgatgcagac agagtttcat
     9481 catgtccgca ctctcaagat catgagtggt gtgtacagcc aggggatgat ggcggatctg
     9541 ctttttgagc agcagatggt agaaaagctg ttcccctgtt tggatgagct gatcagtatc
     9601 catagccaat tcttccagag gattctggag cggaagaagg agtctctggt ggataaaagt
     9661 gaaaagaact ttctcatcaa gaggataggg gatgtgcttg taaatcagtt ttcaggtgag
     9721 aatgcagaac gtttaaagaa gacatatggc aagttttgtg ggcaacataa ccagtctgta
     9781 aactacttca aagaccttta tgccaaggat aagcgttttc aagcctttgt aaagaagaag
     9841 atgagcagtt cagttgttag aaggcttgga attccagagt gcatattgct tgtaactcag
     9901 cggattacca agtacccagt tttattccaa agaatattgc agtgtaccaa agacaatgaa
     9961 gtggagcagg aagatctagc acagtccttg agcctggtga aggatgtgat tggagctgta
    10021 gacagcaaag tggcaagtta tgaaaagaaa gtgcgtctca atgagattta tacaaagaca
    10081 gatagcaagt caatcatgag gatgaagagt ggtcagatgt ttgccaagga agatttgaaa
    10141 cggaagaagc ttgtacgtga tgggagtgtg tttctgaaga atgcagcagg aaggttgaaa
    10201 gaggttcaag cagttcttct cactgacatt ttagttttcc ttcaagaaaa agaccagaag
    10261 tacatctttg catcattgga ccagaagtca acagtgatct ctttaaagaa gctgattgtg
    10321 agagaagtgg cacatgagga gaaaggttta ttcctgatca gcatggggat gacagatcca
    10381 gagatggtag aagtccatgc cagctccaaa gaggaacgaa acagctggat tcagatcatt
    10441 caggacacaa tcaacaccct gaacagagat gaagatgaag gaattcctag tgagaatgag
    10501 gaagaaaaga aaatgttgga caccagagcc cgagaattaa aagaacaact tcaccagaag
    10561 gaccaaaaaa tcctactctt gttggaagag aaggagatga ttttccggga catggctgag
    10621 tgcagcaccc ctctcccaga ggattgctcc ccaacacata gccctagagt tctcttccgc
    10681 tccaacacag aagaggctct caaaggagga cctttaatga aaagtgcaat aaatgaggtg
    10741 gagatccttc agggtttggt gagtggaaat ctgggaggca cacttgggcc gactgtcagc
    10801 agccccattg agcaagatgt ggtcggtccc gtttccctgc cccggagagc agagaccttt
    10861 ggaggatttg acagccatca gatgaatgct tcaaaaggag gcgagaagga agagggagat
    10921 gatggccaag atcttaggag aacggaatca gatagtggcc taaaaaaggg tggaaatgct
    10981 aacctggtat ttatgcttaa aagaaacagt gagcaggttg tccagagcgt tgttcatctc
    11041 tacgagctcc tcagcgctct gcagggtgtg gtgctgcagc aggacagcta cattgaggac
    11101 cagaaactgg tgctgagcga gagggcgctc actcgcagct tgtcccgccc gagctccctc
    11161 attgagcagg agaagcagcg cagcctggag aagcagcgcc aggacctggc caacctgcag
    11221 aagcagcagg cccagtacct cgaggagaag cgcaggcgcg agcgtgagtg ggaagctcgt
    11281 gagagggagc tgcgggagcg ggaggccctc ctggcccagc gcgaggagga ggtgcagcag
    11341 gggcagcagg acctggaaaa ggagcgggag gagctccagc agaagaaggg cacataccag
    11401 tatgacctgg agcgactgcg tgctgcccag aaacagcttg agagggaaca ggagcagctg
    11461 cgccgggagg cagagcggct cagccagcgg cagacagaac gggacctgtg tcagtcccag
    11521 ctttcttgta caaagtggtt cgaggatcca aagtgggaat tccctcggaa gaacttggtt
    11581 cttggaaaaa ctctaggaga aggcgaattt ggaaaagtgg tcaaggcaac ggccttccat
    11641 ctgaaaggca gagcagggta caccacggtg gccgtgaaga tgctgaaaga gaacgcctcc
    11701 ccgagtgagc ttcgagacct gctgtcagag ttcaacgtcc tgaagcaggt caaccaccca
    11761 catgtcatca aattgtatgg ggcctgcagc caggatggcc cgctcctcct catcgtggag
    11821 tacgccaaat acggctccct gcggggcttc ctccgcgaga gccgcaaagt ggggcctggc
    11881 tacctgggca gtggaggcag ccgcaactcc agctccctgg accacccgga tgagcgggcc
    11941 ctcaccatgg gcgacctcat ctcatttgcc tggcagatct cacaggggat gcagtatctg
    12001 gccgagatga agctcgttca tcgggacttg gcagccagaa acatcctggt agctgagggg
    12061 cggaagatga agatttcgga tttcggcttg tcccgagatg tttatgaaga ggattcgtac
    12121 gtgaagagga gccagggtcg gattccagtt aaatggatgg caattgaatc cctttttgat
    12181 catatctaca ccacgcaaag tgatgtatgg tcttttggtg tcctgctgtg ggagatcgtg
    12241 accctagggg gaaaccccta tcctgggatt cctcctgagc ggctcttcaa ccttctgaag
    12301 accggccacc ggatggagag gccagacaac tgcagcgagg agatgtactg cctgatgctg
    12361 caatgctgga agcaggagcc ggacaaaagg ccggtgtttg cggacatcag caaagacctg
    12421 gagaagatga tggttaagag gagagactac ttggaccttg cggcgtccac tccatctgac
    12481 tccctgattt atgacgacgg cctctcagag gaggagacac cgctggtgga ctgtaataat
    12541 gcccccctcc ctcgagccct cccttccaca tggattgaaa acaaactcta tggtagaatt
    12601 tcccatgcat ttactagatt ctagcaccca acttttctat acaaagtggt tgatatccag
    12661 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
    12721 cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc ccccccccct
    12781 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
    12841 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
    12901 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
    12961 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
    13021 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
    13081 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
    13141 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
    13201 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
    13261 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
    13321 acacgatgat aatatggcca cacatatgac cgagtacaag cccacggtgc gcctcgccac
    13381 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
    13441 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
    13501 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
    13561 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
    13621 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
    13681 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
    13741 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
    13801 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
    13861 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
    13921 catgacccgc aagcccggtg cctaaatcga tagatcctaa tcaacctctg gattacaaaa
    13981 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
    14041 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
    14101 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
    14161 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
    14221 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
    14281 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
    14341 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
    14401 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
    14461 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
    14521 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc
    14581 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
    14641 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
    14701 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    14761 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    14821 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
    14881 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa
    14941 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
    15001 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
    15061 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    15121 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    15181 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    15241 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    15301 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    15361 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
    15421 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    15481 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    15541 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    15601 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    15661 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    15721 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    15781 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    15841 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    15901 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    15961 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    16021 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    16081 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    16141 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    16201 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    16261 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    16321 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    16381 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    16441 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    16501 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    16561 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    16621 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    16681 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    16741 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    16801 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    16861 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    16921 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    16981 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat
    17041 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    17101 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc
    17161 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    17221 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    17281 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt
    17341 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    17401 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    17461 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    17521 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
