LOCUS       sequence-241828-        1377 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon-optimized version of D. melanogaster Or47a (hOr47a) with
            N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) 
            for improved intracellular trafficking in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, 
            Lavista-Llanos S
  TITLE     Optimization of Insect Odorant Receptor Trafficking and Functional 
            Expression Via Transient Transfection in HEK293 Cells.
  JOURNAL   Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062.
  PUBMED    31504297
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Senses"; date: "2019-10-26- 26"; volume: "44"; issue: "9"; 
            pages: "673-682"
COMMENT     Sequence Label: pcDNA3.1(-)-mRho.V5.mER.hOr47a
FEATURES             Location/Qualifiers
     source          1..1377
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             85..126
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 acgactcact atagggagac ccaagctggc tagcgtttaa acgggccctc tagagccgcc
       61 accatggatc aagtcgctcc agcaggaaaa cccatcccaa atcctcttct ggggcttgat
      121 agcaccgtta acaagtttag tttggactct ttcctccaag tgcagaaatc cacaattgcc
      181 ctgctgggct ttgatctgtt ctcagaaaac cgcgagatgt ggaagcgacc ctatcgagcc
      241 atgaacgtgt tctctattgc ggccatattc ccgttcattc tcgctgcagt gctccacaat
      301 tggaagaatg tgctgcttct cgctgatgcc atggttgcac tcctgatcac gattcttgga
      361 ctgttcaaat tcagcatgat tctgtacttg agaagagact ttaaacgcct catcgataag
      421 ttccggctgc ttatgtctaa tgaggcggag caaggggagg agtacgctga aattctgaat
      481 gcagcaaaca aacaggatca gaggatgtgt acgctgtttc gtacttgctt tctgcttgct
      541 tgggctttga actctgtgct gcccctggtg aggatgggac tgtcctattg gctggcaggc
      601 catgctgaac cagaactgcc gtttccttgt ctgtttccat ggaacattca catcattcgg
      661 aattatgtgc tgtcattcat ctggtcagcc tttgcctcta caggcgtagt actgcctgcc
      721 gttagcttgg acaccatctt ttgctccttt accagtaacc tctgcgcatt cttcaagatc
      781 gcccagtaca aagtcgtcag gtttaagggc ggaagtctga aagagagcca agccactttg
      841 aacaaggtgt tcgctctgta tcagacctca ttggacatgt gcaatgacct gaaccagtgc
      901 taccagccca tcatctgtgc ccagttcttt ataagctcac tccagctgtg tatgcttggc
      961 tatctgttta gcatcacctt tgcccagaca gaaggtgtct actatgcgtc cttcatcgcc
     1021 acaatcatta ttcaggccta catatactgc tattgtgggg agaatctcaa gactgaatcc
     1081 gccagctttg agtgggcgat ctatgacagt ccttggcacg aatctctggg tgcaggaggt
     1141 gccagcactt ccatatgcag gagcctgctc atcagcatga tgagagcaca tcggggcttc
     1201 cggattaccg ggtacttctt cgaggctaac atggaggctt tcagttccat agtgagaaca
     1261 gccatgtcct acatcactat gctgcgcagc ttttcttgac tcgagcggcc gccactgtgc
     1321 tggatatctg cagaattcca ccacactgga ctagtggatc cgagctcggt accaagc
//
