LOCUS       sequence-241830-        1330 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon-optimized version of D. melanogaster Or47a (hOr47a) with
            an N-terminal tag derived from human HCN1 (mER) for improved 
            intracellular trafficking in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, 
            Lavista-Llanos S
  TITLE     Optimization of Insect Odorant Receptor Trafficking and Functional 
            Expression Via Transient Transfection in HEK293 Cells.
  JOURNAL   Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062.
  PUBMED    31504297
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Senses"; date: "2019-10-26- 26"; volume: "44"; issue: "9"; 
            pages: "673-682"
COMMENT     Sequence Label: pcDNA3.1(-)-V5.mER.hOr47a
FEATURES             Location/Qualifiers
     source          1..1330
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..69
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gggccctcta gagccgccac catggatgga aaacccatcc caaatcctct tctggggctt
       61 gatagcaccg ttaacaagtt tagtttggac tctttcctcc aagtgcagaa atccacaatt
      121 gccctgctgg gctttgatct gttctcagaa aaccgcgaga tgtggaagcg accctatcga
      181 gccatgaacg tgttctctat tgcggccata ttcccgttca ttctcgctgc agtgctccac
      241 aattggaaga atgtgctgct tctcgctgat gccatggttg cactcctgat cacgattctt
      301 ggactgttca aattcagcat gattctgtac ttgagaagag actttaaacg cctcatcgat
      361 aagttccggc tgcttatgtc taatgaggcg gagcaagggg aggagtacgc tgaaattctg
      421 aatgcagcaa acaaacagga tcagaggatg tgtacgctgt ttcgtacttg ctttctgctt
      481 gcttgggctt tgaactctgt gctgcccctg gtgaggatgg gactgtccta ttggctggca
      541 ggccatgctg aaccagaact gccgtttcct tgtctgtttc catggaacat tcacatcatt
      601 cggaattatg tgctgtcatt catctggtca gcctttgcct ctacaggcgt agtactgcct
      661 gccgttagct tggacaccat cttttgctcc tttaccagta acctctgcgc attcttcaag
      721 atcgcccagt acaaagtcgt caggtttaag ggcggaagtc tgaaagagag ccaagccact
      781 ttgaacaagg tgttcgctct gtatcagacc tcattggaca tgtgcaatga cctgaaccag
      841 tgctaccagc ccatcatctg tgcccagttc tttataagct cactccagct gtgtatgctt
      901 ggctatctgt ttagcatcac ctttgcccag acagaaggtg tctactatgc gtccttcatc
      961 gccacaatca ttattcaggc ctacatatac tgctattgtg gggagaatct caagactgaa
     1021 tccgccagct ttgagtgggc gatctatgac agtccttggc acgaatctct gggtgcagga
     1081 ggtgccagca cttccatatg caggagcctg ctcatcagca tgatgagagc acatcggggc
     1141 ttccggatta ccgggtactt cttcgaggct aacatggagg ctttcagttc catagtgaga
     1201 acagccatgt cctacatcac tatgctgcgc agcttttctt gactcgagcg gccgccactg
     1261 tgctggatat ctgcagaatt ccaccacact ggactagtgg atccgagctc ggtaccaagc
     1321 ttaagtttaa
//
