LOCUS       sequence-241841-        1572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human codon optimized E.coli CRISPR Type I-E CasA with 
            N-terminal fusion of Flag tag epitope and nuclear localization 
            signal driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pcDNA-Flag-EcoCas8e
FEATURES             Location/Qualifiers
     source          1..1572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattaca aagacgatga cgataagatg gcgcctaaga agaagcgcaa ggtgcggggc
       61 atggccaatc tcctgattga caattggatc cctgtgagac cgaggaacgg agggaaggtt
      121 cagatcatca acctgcaaag tctctactgt agcagagatc aatggcgact ctcattgccc
      181 agggatgata tggaattggc ggcacttgca ctcttggtat gtattgggca gatcatcgcc
      241 cctgccaagg atgatgtcga gtttaggcac agaataatga accctcttac tgaagatgaa
      301 ttccagcaat tgatcgcgcc ttggatagat atgttctatt tgaatcacgc cgaacatccc
      361 ttcatgcaga caaaaggggt taaagcaaat gatgtcacac caatggagaa gcttttggca
      421 ggtgtgtcag gagcaaccaa ctgcgctttc gttaaccaac caggccaagg tgaggcgttg
      481 tgcggggggt gcacggccat agcgttgttt aatcaggcga accaggctcc tggctttgga
      541 ggaggattca agtccggttt gcgcgggggt acacccgtaa ccacattcgt tcgcggaatt
      601 gacctccgat caacagtcct gttgaacgta ttgacgttgc ctagacttca gaagcaattt
      661 ccaaacgagt ctcacactga gaatcaaccg acttggatta agcccatcaa gtcaaatgaa
      721 agtatacccg cgtcttctat tggatttgtt agaggacttt tttggcagcc cgctcacata
      781 gaactctgtg atcccatagg cattggaaag tgttcttgct gtggccaaga gtctaacctt
      841 agatatacgg gctttctcaa ggagaagttc acgttcaccg tgaatggact ttggccacat
      901 ccccatagcc cgtgcttggt cacggtcaag aagggggagg ttgaagagaa atttcttgcg
      961 tttaccacat ccgccccatc ctggacgcaa atatctcgcg tcgttgtgga taaaatcatc
     1021 cagaacgaaa atggtaaccg cgttgccgcg gtagtgaatc aattcagaaa catagcacca
     1081 cagagcccgt tggagttgat aatgggcggg taccgcaata atcaagcttc cattctcgag
     1141 cgacgccatg acgtacttat gtttaaccag gggtggcagc aatatggtaa cgtgataaac
     1201 gagatagtca cagtcggcct cggctacaaa actgcgttgc gcaaagctct gtatacgttc
     1261 gcggagggtt ttaagaataa agactttaaa ggcgctgggg tgagtgtgca cgagacggca
     1321 gaacgccatt tctatcgcca gtctgaactc ttgattcccg acgttctggc gaatgtcaat
     1381 ttttcccagg ccgatgaggt cattgcggat ctgcgagata agcttcacca gttgtgtgag
     1441 atgctgttca accagtctgt ggctccttat gctcatcatc ccaaactgat ttcaactctc
     1501 gctttggcaa gggctacgtt gtataaacac cttagagaac tgaagccaca gggtggtccc
     1561 agcaatggct ga
//
