LOCUS       sequence-241843-         543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human codon optimized E.coli CRISPR Type I-E CasB with 
            N-terminal fusion of Flag tag epitope and nuclear localization 
            signal driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pcDNA-Flag-EcoCse2
FEATURES             Location/Qualifiers
     source          1..543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattaca aagacgatga cgataagatg gcgcctaaga aaaagaggaa agtacgcggg
       61 atggccgatg aaatagacgc aatggctctt taccgagcat ggcaacagct cgacaatgga
      121 tcttgcgccc aaatacggcg ggtaagtgaa cccgatgaac tgcgagatat ccccgcattc
      181 taccgattgg ttcagccgtt tggctgggag aacccacggc accagcaggc gcttcttagg
      241 atggtttttt gtcttagtgc cgggaaaaac gtaatccgcc atcaggataa gaagtccgaa
      301 caaacaacag ggatttctct gggaagagcg cttgctaaca gcggcaggat caatgaacgc
      361 cgcatatttc agttgatccg agcagatcgg actgctgata tggtccagct caggaggctc
      421 cttacgcacg cagagccagt gttggattgg ccactcatgg caagaatgct tacgtggtgg
      481 gggaagaggg aaaggcagca actgcttgaa gattttgtat tgacgacgaa caaaaacgcg
      541 taa
//
