LOCUS       sequence-241845-        1152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human codon optimized E.coli CRISPR Type I-E CasC with 
            N-terminal fusion of Flag tag epitope and nuclear localization 
            signal driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pcDNA-Flag-EcoCas7
FEATURES             Location/Qualifiers
     source          1..1152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattaca aagacgatga cgataagatg gcgcctaaga agaagcgcaa ggtgagagga
       61 atgtcaaact tcattaatat ccacgtgctt atctcacact cccctagttg ccttaacaga
      121 gacgatatga acatgcaaaa agacgcaatt tttggcggca aaaggagagt cagaattagt
      181 agccagagcc tgaagcgcgc tatgaggaaa agcggctact atgctcaaaa cattggtgaa
      241 agttcattgc ggaccatcca tctcgcgcag ttgagggacg tcctgcgaca gaagcttggg
      301 gaaagatttg atcagaagat catcgacaaa acgcttgccc ttctgtccgg taaatcagtg
      361 gacgaagcgg agaagataag tgcggatgct gttacgccat gggtggtagg tgaaatcgcg
      421 tggttttgcg agcaggtagc caaggccgaa gcggataatt tggatgataa gaaactgctc
      481 aaagtcctca aagaggacat cgcggcgatc cgggtgaacc ttcagcaggg tgttgatatt
      541 gcgctctctg gtcggatggc cacgtctgga atgatgactg aactgggtaa ggtggacgga
      601 gctatgtcta tagctcatgc tataactacc catcaggtgg attctgacat agactggttc
      661 acggctgtcg acgatctcca ggaacaagga tccgcacacc tcggcacgca agaattttct
      721 tctggagtgt tctataggta tgccaacatc aaccttgcac agctccagga aaacctcggt
      781 ggggcaagcc gggaacaggc tcttgaaata gctacccatg tggttcacat gctggcgacc
      841 gaagtgccag gggccaagca gagaacgtac gccgcattca atccggcgga catggtcatg
      901 gtgaatttct ctgatatgcc cttgtctatg gcaaatgctt tcgagaaggc ggtcaaggca
      961 aaggacggtt ttttgcaacc ctccatccaa gcctttaatc agtactggga tagagtagct
     1021 aacgggtatg gtctcaatgg cgcggctgct cagttttctt tgtccgatgt ggatccgata
     1081 acggcgcagg ttaaacagat gcccaccttg gaacaactca aatcctgggt tagaaacaat
     1141 ggggaggcgt ga
//
