LOCUS       sequence-241847-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human codon optimized E.coli CRISPR Type I-E CasD with 
            N-terminal fusion of Flag tag epitope and nuclear localization 
            signal driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pcDNA-Flag-EcoCas5
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattaca aagacgatga cgataagatg gcgcctaaga aaaagaggaa agtacggggc
       61 atgcgatcat acttgatcct gcggcttgca ggtcctatgc aagcctgggg gcaacctacc
      121 tttgaaggta ctcggccgac tggcaggttc cctacgcggt ctggtttgct cggactcctc
      181 ggcgcctgtt tggggataca aagggatgac acttcttcct tgcaggcact ttccgaatca
      241 gtccagttcg cagtgagatg tgatgaactc atactggacg acagacgggt gtccgtaact
      301 ggactgaggg actatcatac tgtactcggc gcaagagaag attatcgagg tcttaagtca
      361 catgagacta ttcagacatg gagggaatat ttgtgtgacg cctccttcac ggtggccctc
      421 tggcttacac cacatgcaac tatggtgatc tcagagcttg agaaagccgt tcttaaacct
      481 cggtacacac catatctggg gaggcggtct tgcccactta cccacccgct tttcttgggg
      541 acttgtcagg ccagcgatcc acagaaggcc ttgctgaact atgaacccgt tggtggcgat
      601 atatacagtg aagagagcgt cacgggccat cacttgaagt tcactgctag ggatgagccg
      661 atgattacgc tcccgagaca gttcgctagt agggaatggt acgttattaa ggggggaatg
      721 gacgtttccc aatga
//
