LOCUS       sequence-241849-         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag 
            tag epitope and nuclear localization signal driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pcDNA-Flag-EcoCas6
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattaca aagacgatga cgataagatg gcgcctaaga agaagcgcaa ggtgcggggc
       61 atgtatctca gtaaagtcat cattgccagg gcctggagca gggatcttta ccaacttcac
      121 cagggattat ggcatttatt tccaaacaga ccggatgctg ctcgtgattt tctttttcat
      181 gttgagaagc gaaacacacc agaaggctgt catgttttat tgcagtcagc gcaaatgcct
      241 gtttcaactg ccgttgcgac agtcattaaa actaaacagg ttgaatttca acttcaggtt
      301 ggtgttccac tctattttcg gcttcgggca aatccgatca aaactattct cgacaatcaa
      361 aagcgcctgg acagtaaagg gaatattaaa cgctgtcggg ttccgttaat aaaagaagca
      421 gaacaaatcg cgtggttgca acgtaaattg ggcaatgcgg cgcgcgttga agatgtgcat
      481 cccatatcgg aacggccaca gtatttttct ggtgatggta aaagtggaaa gatccaaacg
      541 gtttgctttg aaggtgtgct caccatcaac gacgcgccag cgttaataga tcttgtacag
      601 caaggtattg ggccagctaa atcgatggga tgtggcttgc tatctttggc tccactgtga
//
