LOCUS       sequence-241851-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag 
            epitope and NLS and C-terminal fusion of NLS, human p300 HAT core 
            (aa1048-1664), and Flag tag epitope driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pcDNA-CasE-p300-Flag
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..69
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgggtgccc cagttcccta tcccgaccca ctggagccaa ggatggcgcc taagaagaag
       61 cgcaaggtgc ggggcatgta tctcagtaaa gtcatcattg ccagggcctg gagcagggat
      121 ctttaccaac ttcaccaggg attatggcat ttatttccaa acagaccgga tgctgctcgt
      181 gattttcttt ttcatgttga gaagcgaaac acaccagaag gctgtcatgt tttattgcag
      241 tcagcgcaaa tgcctgtttc aactgccgtt gcgacagtca ttaaaactaa acaggttgaa
      301 tttcaacttc aggttggtgt tccactctat tttcggcttc gggcaaatcc gatcaaaact
      361 attctcgaca atcaaaagcg cctggacagt aaagggaata ttaaacgctg tcgggttccg
      421 ttaataaaag aagcagaaca aatcgcgtgg ttgcaacgta aattgggcaa tgcggcgcgc
      481 gttgaagatg tgcatcccat atcggaacgg ccacagtatt tttctggtga tggtaaaagt
      541 ggaaagatcc aaacggtttg ctttgaaggt gtgctcacca tcaacgacgc gccagcgtta
      601 atagatcttg tacagcaagg tattgggcca gctaaatcga tgggatgtgg cttgctatct
      661 ttggctccac tg
//
