LOCUS       sequence-24186-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  TITLE     T4 Lysozyme Plasmids - Tray 3
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme K83M T115E
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(713..735)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    724..740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(748..754,755..770,771..775))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ctangnaaaa gcccgctcat taggcgggct cagatctgct catgttttga cagcttatca
       61 tcgataagct tcggcaataa attcttgata tgttttcata tgtttccttt aaatatttta
      121 attagtaatt gtctattcaa gtaattgtga atatactatc acaattccaa gagaaagtaa
      181 acagctttat agatttttat acgcgtccca agtgccagtt ctaaacgttg taatgactcg
      241 ttttgcgcga ttaggtgttt gattatacca tctactttta gctaagttaa ctgctgcttc
      301 atcccagcgt ttttgttgaa gcatacgtaa agagttctca aatcctgcca caccggtttc
      361 tcccatttgg aaaaccatat taatcaatgc acagcgacga accgcatcaa gagaatcata
      421 aaccggtttt aacatagcat ttctcagaat tccgcgaaca gcagcatcaa catcctgatt
      481 aaagagtttt tcagcctcat cttttgtaat tacaccattg caattacgcc caatagcttt
      541 atctaattca gatttagcag cattaagtga tggacttttt gtaagcaaat gaccgatgcc
      601 aatagtgtaa tagccttctg tgtctttata gattttaagt ctaagacctt catctatacg
      661 taacatttca aatatattca taatacctcc taagcatcga tggatcctgt ttcctgtgtg
      721 aaattgttat ccgctcacaa ttccacacat tatacgagcc gatgattaat tgtcancagg
      781 gggatgggga gta
//
