LOCUS       sequence-241864-        5709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for the functional expression of insect ORs in mammalian 
            cells, with a human codon-optimized version of D.melanogaster Orco 
            (hOrco) with an N-terminal myc tag and a beta-globin/IgG chimeric 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, 
            Lavista-Llanos S
  TITLE     Optimization of Insect Odorant Receptor Trafficking and Functional 
            Expression Via Transient Transfection in HEK293 Cells.
  JOURNAL   Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062.
  PUBMED    31504297
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Senses"; date: "2019-10-26- 26"; volume: "44"; issue: "9"; 
            pages: "673-682"
COMMENT     Sequence Label: pDmelOR
FEATURES             Location/Qualifiers
     source          1..5709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    110..191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        494..797
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        798..1001
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     952..972
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1035..1044
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1044..1073
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          2289..2421
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2404..2423
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    2802..2883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2894..2911)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      3019..3474
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3106..3125)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3316..3337
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3541..3560)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3660..3682
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3720..3738)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3806..3910
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3911..3979,3980..4771)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4129..4148)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4942..5530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5431..5450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
       61 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcgg cagtgaaaaa
      121 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
      181 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
      241 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc
      301 tagactgcag cctcaggaga tctgggcccc cgcggcatat gaccggtgaa ttctccaggc
      361 gatctgacgg ttcactaaac gagctctgct tatataggcc tcccaccgta cacgccacct
      421 cgacatactc gagtagttat taatagtaat caattacggg gtcattagtt catagcccat
      481 atatggagtt ccgcgttaca taacttacgg taaatggctc gcctggctga ccgcccaacg
      541 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      601 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      661 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      721 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      781 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      841 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      901 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      961 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     1021 tccgctaggg atccgccacc atggaacaga aactgatctc tgaagaagac ctggctagca
     1081 caactagcat gcaacccagt aaatatacag gcctggtggc cgatctcatg cccaacataa
     1141 gggcaatgaa atacagcggg ctctttatgc acaactttac gggagggtcc gccttcatga
     1201 agaaagtcta cagcagcgta catctcgttt tccttctgat gcagttcacc ttcattctgg
     1261 tcaatatggc ccttaatgcc gaagaggtga atgagctttc tgggaatacc atcaccacac
     1321 tgttcttcac acactgcatc acaaagttca tctacctggc agtgaaccag aagaactttt
     1381 accgtacact gaacatctgg aatcaagtga acacccaccc tctgtttgcc gaatcagatg
     1441 ccagatacca cagtattgca ctcgccaaga tgaggaaact cttctttctt gtgatgctga
     1501 caacagtggc tagtgctact gcctggacca ctatcacgtt ctttggcgat tctgtgaaaa
     1561 tggtggtaga ccatgaaacc aacagcagca tccctgtcga gattccaaga cttccgatta
     1621 agtcctttta tccctggaat gcaagtcatg gcatgttcta catgatctcc ttcgccttcc
     1681 aaatctacta cgtgctgttt agcatgatac acagcaacct ctgtgacgtg atgttctgct
     1741 cttggctgat atttgcctgt gaacagctcc agcatctgaa aggcattatg aagccactga
     1801 tggaactgtc agcctcactt gacacttatc gcccaaattc tgctgctctc tttaggtcac
     1861 tctcagctaa tagcaaatcc gagttgattc acaacgagga gaaagaccct gggactgaca
     1921 tggacatgtc tggcatttat agcagcaaag ccgactgggg agcacagttt cgggcacctt
     1981 ctacactcca gtcctttggc ggtaatggag gaggaggtaa tgggctggtt aacggtgcta
     2041 accccaacgg tctgaccaag aaacaggaga tgatggtgcg atctgcgatc aagtactggg
     2101 tcgaacgcca taagcacgtg gttagactgg ttgctgctat aggcgatacc tacggtgccg
     2161 ctttgctgtt gcatatgctg actagcacga tcaagttgac tctgttggca tatcaagcca
     2221 ccaagatcaa tggagtgaac gtatatgcgt ttacggttgt ggggtatctg ggctatgctc
     2281 tggcccaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
     2341 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
     2401 actttgcctt tctctccaca ggtgtttcac ttttgcattt tcggcaatcg gctgatagag
     2461 gagagttcct ctgttatgga ggcagcgtac agttgccact ggtacgatgg aagcgaagaa
     2521 gccaagactt tcgttcagat tgtgtgtcag cagtgtcaga aggctatgtc catctcaggg
     2581 gcgaaattct tcaccgtgtc cttggatctg tttgcctccg tactgggcgc agtcgtcacc
     2641 tatttcatgg tcctggtcca actgaagtga aagcttgtcg acgatatctc cagaggatca
     2701 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     2761 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     2821 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2881 tgccccgagc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2941 tatcagctca ctcaaaggcg gtaatacggt tatccagatc gcccttccca acagttgcgc
     3001 agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3061 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3121 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     3181 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     3241 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     3301 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     3361 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3421 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttcctgat
     3481 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     3541 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     3601 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3661 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     3721 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3781 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3841 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3901 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3961 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4021 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4081 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4141 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4201 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4261 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4321 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4381 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4441 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4501 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4561 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4621 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4681 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4741 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4801 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4861 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5581 tgctcacatg gctcgacaga tcttcaatat tggccattag ccatattatt cattggttat
     5641 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
     5701 tacatttat
//
