LOCUS       sequence-241872-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion 
            of Flag tag epitope and nuclear localization signal driven by CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pcDNA-Flag-LmoCas6
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattaca aagacgacga tgataagatg gcccccaaga agaagagaaa ggtccgcggc
       61 atgagattaa agataaattg tgattttgat tctaaaataa tcccaaaaga ttttcaaagt
      121 aaagttgtta gtctattcaa agcaggaatt atgaaatcga gtcctgagag atatgagaat
      181 ttatttggcg gaaataagca taagcaatat acattttcag tgtaccttcc caaacctcaa
      241 aataaaggtg cggaaattca gttaaatgaa gcaaattgta ttattaattt ttcaacggga
      301 gatgcggaaa cgggaatcat tttttataat gctttgatca gtttgagagg tagcaaagtt
      361 ttatttggag ctgggaacca tattactgta aaaaacatcc aaatcgttcc agagaaaaaa
      421 attatcggca aacaaacaat tttgagaacg ctttcacctg tggttagtag agatcataat
      481 agagaaacat tcaagaattg gttttacagt tttgaggatg aggaatttga gccaactttg
      541 aaaagaaata tgttgcctta tttaatagat gcatttggtg agcaagctcg ttatgatttg
      601 gagaaattga aaataacgcc aatttcgatg aagaaagtag tcgtttattg ccatgagata
      661 catattgaaa gttcagttgg gatttttgaa ttggaggctg aggcatattt gcagaagtat
      721 ttggtggaga atgggattgg gactatgaca ggatctggat ttggcatgat agagcagttt
      781 tag
//
