LOCUS       sequence-241879-        1749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B 
            Flag-Cas8b2 driven by hUbC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pLenti-LmoCas8b2
FEATURES             Location/Qualifiers
     source          1..1749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattaca aggacgatga cgacaaaatg gcaccgaaga agaagcggaa ggtccgcgga
       61 atgcagaccg aaatcgaagt cagggcagac gactggctca tcaacaccgg actgaccggc
      121 ttcctgaaca ttgtgggaaa agagaacgtc cggattgacg ggcagtctat ctacttcacc
      181 gctgacctcc tggaagattt cgaaactaag tactttaact tcttcatcca agagtacaaa
      241 gaaactctct catggtacaa gatcgtcagc tacaaagaga ctatggagca gttcagaatt
      301 gatgaattcg cgtcctttga cgaagtggcc ctggggaacc tgaacaagta catgaaggac
      361 gtcgtgaagt tctatctgaa aaaggccaac tatatcaaag tgttcccctt gattgaccct
      421 agcgccaacg tgaccaagtg gctcgaaaag ctgaacacca ttaatctgac caagaagcag
      481 aaatgggatg aggtcaaggc tgacattatt gaggaggtca aacagaccta cacccaactc
      541 gacctgatta ttgacttctg tgcctccgaa aagggactgc agtacctggg agccaagaac
      601 ctgatctatt ccgtcattaa caagggctgg agcggagtct ccttcctgtt caagcaaacc
      661 aagttcattg acccgtatct ggactacaag acgaccttcg tggatcctgt gctggagtac
      721 ctggataccg atctgtccaa ggccaagtac aactgcttca actgcaacca gcccatcaag
      781 aacctcaagc tcgacctctc gttcatgaat gacgtgggct ttgacaccgc ccgcaagact
      841 gcgcacgtct gggatttcaa caacgatgtg gccacttgtc caatctgccg gctgatctac
      901 tcatgcgtgc ctgccgggtt cacctacgtg tatggagagg gaatgttcgt gaacgactcc
      961 tacggcgtgg aagaactgca cagagtgaac gaacggatgc ggaacagcat cctgaggttt
     1021 aacaaggacg gtatcaattc caccaacact taccgcgcgc ttgtggagtc cattaccatg
     1081 gaacacgaaa acaacagacg ctacgaattg gctgacatcc agctcgtgcg atacgagaac
     1141 gagcattacc ggttcaatct gttgtcaaag aagatgcttc acatcgtgaa cgattccaag
     1201 ggtatcctga agtcgctgat acgctgcggt tacaaggagg ggaacctcaa cgtgaacctc
     1261 tacaaggaag tgatccagca tctgatgaac aacgaaaacc tcttcaccct tatccacaag
     1321 ttgatctact acaagcagac cagcgtgaac ggcctgtact acaacatggg ccacgtgtcc
     1381 ggaatcctgg atatcaacac caagttcctg aaagaaatgg aggtcatgac gaacatctca
     1441 cagaaccagc tgtggttcgt gcaaaattgt ggcaaagaat tcaaggaggg ttacgtgaag
     1501 aagaagtccg agaacaagat ctccggaatc acttacaagc tgctgaattc gctgaaggtc
     1561 aacgacaagg acggattcat ggacaccctg ctgaactcct actcgtacct gtccatgccg
     1621 atccccgacg tgttcatcaa gatgttctcc aacaacgaag ccttcaagag cgtgggctac
     1681 gcttttatgc ttggcgtggg tggagaaaag actaagaagg aagatggggg caacaccgat
     1741 gagaagtga
//
