LOCUS       sequence-241884-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B 
            Flag-Cas5 driven by hUbC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pLenti-LmoCas5
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             34..54
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggactata aggatgatga cgacaagatg gcccccaaga agaagcgcaa agtccgcggg
       61 atgaaggcta ttagggtccg gctgtggcag gatctcgtga acttcaagaa gcctaccagc
      121 ttccaactga aggaaaccta ccctctgccg ccgtactcta ccgtgatcgg catggtgcat
      181 acactgtgcg gctttacctc ctaccacgag atgaagatca gcattcaggg aaagtacttt
      241 agcaaggtca acgacctcgc gactagatac gaattcaaga atggctcaaa atacgacgtg
      301 gaacgccacc agatcaaagt ggacaaatac ggagtgtcga gaggcgtcag caccgtggag
      361 ctccttgtgg acctcgaact gctgctgcac atcattccgg aagatccatc cttggtgccg
      421 atcattgaaa aggcattccg ggaacccatc gagttccctt ccctgggacg cagagaggac
      481 atcgccacga ttcagaaggt cgaagtggtg gacgtcgaga agcgacagcc caagaagtcc
      541 accgagatct ccgacggtta taacgcctac gtgccgatca gcttgaccga gaacaactcg
      601 gtgcatttca agtcccacga gtcctcagtg ggacgggata agctgctggg gactcggtac
      661 cttctgactg agaaatacga gcgcgtgaac cacggaaccg aaaaggcccc taagttcttc
      721 cggaagtggc ggaagaagga cgtcatctac tcctcgcgca tcttcgtgtc caagaaggat
      781 gtgttctttt tcgacgagga cgattacctg gtgttcatcg aagaagaaga gtga
//
