LOCUS       sequence-241900-        1325 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon-optimized version of D. melanogaster Or47a (hOr47a) 
            without N-terminal tags for improved intracellular trafficking in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, 
            Lavista-Llanos S
  TITLE     Optimization of Insect Odorant Receptor Trafficking and Functional 
            Expression Via Transient Transfection in HEK293 Cells.
  JOURNAL   Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062.
  PUBMED    31504297
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Senses"; date: "2019-10-26- 26"; volume: "44"; issue: "9"; 
            pages: "673-682"
COMMENT     Sequence Label: pcDNA3.1(-)-V5.hOr47a
FEATURES             Location/Qualifiers
     source          1..1325
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..75
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ttaaacgggc cctctagagc cgccaccatg gatggaaaac ccatcccaaa tcctcttctg
       61 gggcttgata gcaccgactc tttcctccaa gtgcagaaat ccacaattgc cctgctgggc
      121 tttgatctgt tctcagaaaa ccgcgagatg tggaagcgac cctatcgagc catgaacgtg
      181 ttctctattg cggccatatt cccgttcatt ctcgctgcag tgctccacaa ttggaagaat
      241 gtgctgcttc tcgctgatgc catggttgca ctcctgatca cgattcttgg actgttcaaa
      301 ttcagcatga ttctgtactt gagaagagac tttaaacgcc tcatcgataa gttccggctg
      361 cttatgtcta atgaggcgga gcaaggggag gagtacgctg aaattctgaa tgcagcaaac
      421 aaacaggatc agaggatgtg tacgctgttt cgtacttgct ttctgcttgc ttgggctttg
      481 aactctgtgc tgcccctggt gaggatggga ctgtcctatt ggctggcagg ccatgctgaa
      541 ccagaactgc cgtttccttg tctgtttcca tggaacattc acatcattcg gaattatgtg
      601 ctgtcattca tctggtcagc ctttgcctct acaggcgtag tactgcctgc cgttagcttg
      661 gacaccatct tttgctcctt taccagtaac ctctgcgcat tcttcaagat cgcccagtac
      721 aaagtcgtca ggtttaaggg cggaagtctg aaagagagcc aagccacttt gaacaaggtg
      781 ttcgctctgt atcagacctc attggacatg tgcaatgacc tgaaccagtg ctaccagccc
      841 atcatctgtg cccagttctt tataagctca ctccagctgt gtatgcttgg ctatctgttt
      901 agcatcacct ttgcccagac agaaggtgtc tactatgcgt ccttcatcgc cacaatcatt
      961 attcaggcct acatatactg ctattgtggg gagaatctca agactgaatc cgccagcttt
     1021 gagtgggcga tctatgacag tccttggcac gaatctctgg gtgcaggagg tgccagcact
     1081 tccatatgca ggagcctgct catcagcatg atgagagcac atcggggctt ccggattacc
     1141 gggtacttct tcgaggctaa catggaggct ttcagttcca tagtgagaac agccatgtcc
     1201 tacatcacta tgctgcgcag cttttcttga ctcgagcggc cgccactgtg ctggatatct
     1261 gcagaattcc accacactgg actagtggat ccgagctcgg taccaagctt aagtttaaac
     1321 cgctg
//
