LOCUS       sequence-241901-        1668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for the functional expression of insect ORs in mammalian 
            cells, with a human codon-optimized version of D.melanogaster Orco 
            (hOrco) with an N-terminal myc tag and a beta-globin/IgG chimeric 
            intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, 
            Lavista-Llanos S
  TITLE     Optimization of Insect Odorant Receptor Trafficking and Functional 
            Expression Via Transient Transfection in HEK293 Cells.
  JOURNAL   Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062.
  PUBMED    31504297
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Senses"; date: "2019-10-26- 26"; volume: "44"; issue: "9"; 
            pages: "673-682"
COMMENT     Sequence Label: pDmelOR
FEATURES             Location/Qualifiers
     source          1..1668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             19..48
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          1264..1396
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1379..1398
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 tagggatccg ccaccatgga acagaaactg atctctgaag aagacctggc tagcacaact
       61 agcatgcaac ccagtaaata tacaggcctg gtggccgatc tcatgcccaa cataagggca
      121 atgaaataca gcgggctctt tatgcacaac tttacgggag ggtccgcctt catgaagaaa
      181 gtctacagca gcgtacatct cgttttcctt ctgatgcagt tcaccttcat tctggtcaat
      241 atggccctta atgccgaaga ggtgaatgag ctttctggga ataccatcac cacactgttc
      301 ttcacacact gcatcacaaa gttcatctac ctggcagtga accagaagaa cttttaccgt
      361 acactgaaca tctggaatca agtgaacacc caccctctgt ttgccgaatc agatgccaga
      421 taccacagta ttgcactcgc caagatgagg aaactcttct ttcttgtgat gctgacaaca
      481 gtggctagtg ctactgcctg gaccactatc acgttctttg gcgattctgt gaaaatggtg
      541 gtagaccatg aaaccaacag cagcatccct gtcgagattc caagacttcc gattaagtcc
      601 ttttatccct ggaatgcaag tcatggcatg ttctacatga tctccttcgc cttccaaatc
      661 tactacgtgc tgtttagcat gatacacagc aacctctgtg acgtgatgtt ctgctcttgg
      721 ctgatatttg cctgtgaaca gctccagcat ctgaaaggca ttatgaagcc actgatggaa
      781 ctgtcagcct cacttgacac ttatcgccca aattctgctg ctctctttag gtcactctca
      841 gctaatagca aatccgagtt gattcacaac gaggagaaag accctgggac tgacatggac
      901 atgtctggca tttatagcag caaagccgac tggggagcac agtttcgggc accttctaca
      961 ctccagtcct ttggcggtaa tggaggagga ggtaatgggc tggttaacgg tgctaacccc
     1021 aacggtctga ccaagaaaca ggagatgatg gtgcgatctg cgatcaagta ctgggtcgaa
     1081 cgccataagc acgtggttag actggttgct gctataggcg atacctacgg tgccgctttg
     1141 ctgttgcata tgctgactag cacgatcaag ttgactctgt tggcatatca agccaccaag
     1201 atcaatggag tgaacgtata tgcgtttacg gttgtggggt atctgggcta tgctctggcc
     1261 caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact gggcttgtcg
     1321 agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga catccacttt
     1381 gcctttctct ccacaggtgt ttcacttttg cattttcggc aatcggctga tagaggagag
     1441 ttcctctgtt atggaggcag cgtacagttg ccactggtac gatggaagcg aagaagccaa
     1501 gactttcgtt cagattgtgt gtcagcagtg tcagaaggct atgtccatct caggggcgaa
     1561 attcttcacc gtgtccttgg atctgtttgc ctccgtactg ggcgcagtcg tcacctattt
     1621 catggtcctg gtccaactga agtgaaagct tgtcgacgat atctccag
//
