LOCUS       sequence-241902-        1284 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon-optimized D. melanogaster Orco (hOrco) and Or47a 
            (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin 
            (mRho) and HCN1 (mER) for improved trafficking in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, 
            Lavista-Llanos S
  TITLE     Optimization of Insect Odorant Receptor Trafficking and Functional 
            Expression Via Transient Transfection in HEK293 Cells.
  JOURNAL   Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062.
  PUBMED    31504297
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Senses"; date: "2019-10-26- 26"; volume: "44"; issue: "9"; 
            pages: "673-682"
COMMENT     Sequence Label: pDmelOR-mRho.V5.mER.hOr47a
FEATURES             Location/Qualifiers
     source          1..1284
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..78
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctggaggccg ccaccatgga tcaagtcgct ccagcaggaa aacccatccc aaatcctctt
       61 ctggggcttg atagcaccgt taacaagttt agtttggact ctttcctcca agtgcagaaa
      121 tccacaattg ccctgctggg ctttgatctg ttctcagaaa accgcgagat gtggaagcga
      181 ccctatcgag ccatgaacgt gttctctatt gcggccatat tcccgttcat tctcgctgca
      241 gtgctccaca attggaagaa tgtgctgctt ctcgctgatg ccatggttgc actcctgatc
      301 acgattcttg gactgttcaa attcagcatg attctgtact tgagaagaga ctttaaacgc
      361 ctcatcgata agttccggct gcttatgtct aatgaggcgg agcaagggga ggagtacgct
      421 gaaattctga atgcagcaaa caaacaggat cagaggatgt gtacgctgtt tcgtacttgc
      481 tttctgcttg cttgggcttt gaactctgtg ctgcccctgg tgaggatggg actgtcctat
      541 tggctggcag gccatgctga accagaactg ccgtttcctt gtctgtttcc atggaacatt
      601 cacatcattc ggaattatgt gctgtcattc atctggtcag cctttgcctc tacaggcgta
      661 gtactgcctg ccgttagctt ggacaccatc ttttgctcct ttaccagtaa cctctgcgca
      721 ttcttcaaga tcgcccagta caaagtcgtc aggtttaagg gcggaagtct gaaagagagc
      781 caagccactt tgaacaaggt gttcgctctg tatcagacct cattggacat gtgcaatgac
      841 ctgaaccagt gctaccagcc catcatctgt gcccagttct ttataagctc actccagctg
      901 tgtatgcttg gctatctgtt tagcatcacc tttgcccaga cagaaggtgt ctactatgcg
      961 tccttcatcg ccacaatcat tattcaggcc tacatatact gctattgtgg ggagaatctc
     1021 aagactgaat ccgccagctt tgagtgggcg atctatgaca gtccttggca cgaatctctg
     1081 ggtgcaggag gtgccagcac ttccatatgc aggagcctgc tcatcagcat gatgagagca
     1141 catcggggct tccggattac cgggtacttc ttcgaggcta acatggaggc tttcagttcc
     1201 atagtgagaa cagccatgtc ctacatcact atgctgcgca gcttttcttg acaccggtca
     1261 tatgccgcgg gggcccagat ctcc
//
