LOCUS       sequence-241903-        1399 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon-optimized D. melanogaster Orco (hOrco) and Or56a 
            (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin 
            (mRho) and HCN1 (mER) for improved trafficking in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Miazzi F, Hoyer C, Sachse S, Knaden M, Wicher D, Hansson BS, 
            Lavista-Llanos S
  TITLE     Optimization of Insect Odorant Receptor Trafficking and Functional 
            Expression Via Transient Transfection in HEK293 Cells.
  JOURNAL   Chem Senses. 2019 Oct 26;44(9):673-682. doi: 10.1093/chemse/bjz062.
  PUBMED    31504297
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Chem Senses"; date: "2019-10-26- 26"; volume: "44"; issue: "9"; 
            pages: "673-682"
COMMENT     Sequence Label: pDmelOR-mRho.V5.mER.hOr56a
FEATURES             Location/Qualifiers
     source          1..1399
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..75
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctggaggcca ccatggatca agtcgctcca gcaggaaaac ccatcccaaa tcctcttctg
       61 gggcttgata gcaccgttaa caagtttagt ctgttcaagg taaaggactt gttgctctct
      121 cctacgacat tcgaggaccc gatcttcggc acccatctcc gctattttca atggtatggg
      181 tatgtcgcct cgaaggatca aaatcgcccg ttgctcagtc tcatccgctg tacgatttta
      241 acggcatcta tttggctaag ctgcgcacta atgctcgccc gcgttttcag aggctatgaa
      301 aatttgaatg acggtgcgac gtcttacgcg acagctgtgc aatatttcgc ggtgtcaata
      361 gccatgttca acgcttacgt gcaacgtgac aaggtcatct cacttcttcg tgtcgcgcat
      421 tccgacatac agaacctaat gcatgaagcg gataatagag agatggagct tttagtggca
      481 acacaagcat acactcggac gataacttta ctcatatgga taccctcagt aatcgctggc
      541 ctaatggcat attcggactg tatttatcgt tcattatttc tcccaaagtc tgttttcaac
      601 gtgccagctg tgcgtcgcgg cgaggaacat cccatcttgt tgttccagtt gtttccattt
      661 ggggagttgt gtgataactt tgtcgtaggg tatttaggcc cgtggtacgc actcggcctg
      721 gggattactg ctattccact ctggcataca tttataactt gtcttatgaa atacgttaat
      781 ctaaagctgc aaatccttaa caaacgtgtt gaggagatgg acataacacg tctgaacagt
      841 aaacttgtaa taggacgatt gacggcaagt gagttaacct tctggcaaat gcaactgttt
      901 aaggagttcg ttaaggagca gttgaggata cgtaagttcg ttcaagaact gcaatacctc
      961 atctgcgttc ccgtaatggc cgactttatc atattctcgg tgctgatatg tttccttttc
     1021 ttcgctctca cagtcggagt accgtctaaa atggactact tcttcatgtt catatatctt
     1081 ttcgtaatgg cagggatact ctggatatac cattggcacg ctacactcat cgttgagtgt
     1141 cacgacgagt taagccttgc gtatttctcg tgtggatggt ataacttcga gatgcctctc
     1201 cagaagatgt tagtattcat gatgatgcat gcgcagcggc cgatgaaaat gagggcactg
     1261 cttgttgacc taaatctaag aacgttcatt gacataggac gaggtgcgta ctcatacttc
     1321 aatcttttga ggagttctca tctgtactga caccggtcat atgccgcggg ggcccagatc
     1381 tcctgaggct gcagtctag
//
