LOCUS       sequence-241908-        5162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing Nuclear Localized mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5162)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS1-his-24::mCherry
FEATURES             Location/Qualifiers
     source          1..5162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..60
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     52..69
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     53..69
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     79..98
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        79..97
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2427..2447)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2427..2445)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2466..2482)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2466..2482)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2479..2501)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2490..2506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2514..2520,2521..2538,2539..2544))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2559..2580
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2697..2714)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2868..3456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2948..2967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3627..4418,4419..4487))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4250..4269
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4488..4592)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4618..5073)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4755..4776)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4967..4986
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
       61 acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct ccaccgcgga
      121 ctagtggatc ccccgggctg cagcctagga tgtctgattc cgctgttgtt gccgccgctg
      181 tcgagccaaa ggtcccaaag gctaaggccg ccaaggccgc caagccagcc gccaaggccg
      241 ccaagccaac caaagttgcc aaggccaagg ctccagtcgc tcacccacca tacatcaaca
      301 tgatcaagga ggccatcaag cagctcaagg accgcaaagg agcttccaag caggcaatcc
      361 tcaagttcat ctcccaaaac tacaagctcg gagacaatgt catccagatc aatgctcatc
      421 tccgtcaggc tctcaagcgt ggtgtcacca gcaaggccct tgttcaagcc gccggatccg
      481 gagccaacgg acgtttccgt gtgccagaga aggccgccgc cgccaagaag ccagcagcag
      541 ccaagaagcc agcagcagcc aagaaaccag cagccgccaa gaaagccact ggagagaaga
      601 aggcaaagaa gccagccgct gccaagccaa agaaggccgc caccggagat aagaaggtca
      661 agaaggccaa gagcccaaag aaggttgcca agccagctgc caagaaggtc gccaagtctc
      721 cagccaagaa ggccgctcca aagaagatcg ccaagccagc tgccaagaag gccgccaagc
      781 cagccgccaa ggccgcatgc atggtctcaa agggtgaaga agataacatg gcaattatta
      841 aagagtttat gcgtttcaag gtgcatatgg agggatctgt caatgggcat gagtttgaaa
      901 ttgaaggtga aggagaaggc cgaccatatg agggaacaca aaccgcaaaa ctaaaggtaa
      961 gtttaaacat atatatacta actaaccctg attatttaaa ttttcaggta actaaaggcg
     1021 gaccattacc attcgcctgg gacatcctct ctccacagtt catgtatgga agtaaagctt
     1081 atgttaaaca tccggcagat ataccagatt atttgaaact ttcattcccg gagggtttta
     1141 agtgggaacg cgtaatgaat tttgaagacg gaggagttgt tacagtgacg caagactcaa
     1201 ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc agcctccaag
     1261 atggagaatt tatttataaa gtcaaacttc gaggaacgaa tttcccctcg gatggacctg
     1321 ttatgcagaa gaagactatg ggatgggaag cttcaagtga aagaatgtac cctgaagacg
     1381 gtgctcttaa gggagagatt aaacaacgtc ttaaattgaa agatggagga cattacgatg
     1441 ctgaggtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcaggtga
     1501 agacaactta caaagccaaa aaaccagttc agctgccagg agcgtacaat gttaatatta
     1561 aactggatat cacctcccac aacgaggatt acactatcgt tgagcaatat gaaagagctg
     1621 aagggcggca ctcgacaggt ggcatggatg aattgtataa gaagctttgg aattcatctc
     1681 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     1741 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     1801 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     1861 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     1921 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     1981 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     2041 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     2101 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     2161 aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg
     2221 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     2281 gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt
     2341 gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt
     2401 ttgggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     2461 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2521 cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     2581 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     2641 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2701 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2761 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2821 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2881 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2941 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3001 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3061 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3121 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3181 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3241 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3301 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3361 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3421 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3481 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3541 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3601 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3661 gcgatctgcc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3721 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3781 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3841 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3901 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3961 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4021 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4081 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4141 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4201 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4261 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4321 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4381 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4441 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4501 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4561 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     4621 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     4681 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     4741 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     4801 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     4861 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     4921 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     4981 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     5041 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     5101 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     5161 gt
//
