LOCUS       sequence-241909-        4598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing Nuclear Localized mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4598)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS2-his-24::mCherry
FEATURES             Location/Qualifiers
     source          1..4598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     71..93
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     85..102
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     86..102
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     112..131
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        112..130
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1896..1916)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1896..1914)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1935..1951)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1935..1951)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1948..1970)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1959..1975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1983..1989,1990..2007,2008..2013))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2028..2049
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2166..2183)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2337..2925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2417..2436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3096..3887,3888..3956))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3719..3738
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3957..4061)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4087..4542)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4224..4245)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4436..4455
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
       61 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
      121 tcactatagg gcgaattgga gctccaccgc ggactagtgg atcccccggg ctgcagccta
      181 ggatgtctga ttccgctgtt gttgccgccg ctgtcgagcc aaaggtccca aaggctaagg
      241 ccgccaaggc cgccaagcca gccgccaagg ccgccaagcc aaccaaagtt gccaaggcca
      301 aggctccagt cgctcaccca ccatacatca acatgatcaa ggaggccatc aagcagctca
      361 aggaccgcaa aggagcttcc aagcaggcaa tcctcaagtt catctcccaa aactacaagc
      421 tcggagacaa tgtcatccag atcaatgctc atctccgtca ggctctcaag cgtggtgtca
      481 ccagcaaggc ccttgttcaa gccgccggat ccggagccaa cggacgtttc cgtgtgccag
      541 agaaggccgc cgccgccaag aagccagcag cagccaagaa gccagcagca gccaagaaac
      601 cagcagccgc caagaaagcc actggagaga agaaggcaaa gaagccagcc gctgccaagc
      661 caaagaaggc cgccaccgga gataagaagg tcaagaaggc caagagccca aagaaggttg
      721 ccaagccagc tgccaagaag gtcgccaagt ctccagccaa gaaggccgct ccaaagaaga
      781 tcgccaagcc agctgccaag aaggccgcca agccagccgc caaggccgca tgcatggtct
      841 caaagggtga agaagataac atggcaatta ttaaagagtt tatgcgtttc aaggtgcata
      901 tggagggatc tgtcaatggg catgagtttg aaattgaagg tgaaggagaa ggccgaccat
      961 atgagggaac acaaaccgca aaactaaagg taagtttaaa catatatata ctaactaacc
     1021 ctgattattt aaattttcag gtaactaaag gcggaccatt accattcgcc tgggacatcc
     1081 tctctccaca gttcatgtat ggaagtaaag cttatgttaa acatccggca gatataccag
     1141 attatttgaa actttcattc ccggagggtt ttaagtggga acgcgtaatg aattttgaag
     1201 acggaggagt tgttacagtg acgcaagact caaggtaagt ttaaacagtt cggtactaac
     1261 taaccataca tatttaaatt ttcagcctcc aagatggaga atttatttat aaagtcaaac
     1321 ttcgaggaac gaatttcccc tcggatggac ctgttatgca gaagaagact atgggatggg
     1381 aagcttcaag tgaaagaatg taccctgaag acggtgctct taagggagag attaaacaac
     1441 gtcttaaatt gaaagatgga ggacattacg atgctgaggt aagtttaaac atgattttac
     1501 taactaacta atctgattta aattttcagg tgaagacaac ttacaaagcc aaaaaaccag
     1561 ttcagctgcc aggagcgtac aatgttaata ttaaactgga tatcacctcc cacaacgagg
     1621 attacactat cgttgagcaa tatgaaagag ctgaagggcg gcactcgaca ggtggcatgg
     1681 atgaattgta taagaagctt tggaattcgc tagccggcca tacaagtaat ccggatgatc
     1741 gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt ttttcctatt
     1801 atttatttat tctctttgtg ttttttttct tgctttctaa aaaattaatt caatccaaat
     1861 ctaaacatga ggggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc
     1921 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     1981 cacaacatac gagccgggag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     2041 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     2101 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     2161 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2221 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2281 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2341 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2401 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2461 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2521 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2581 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2641 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2701 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2761 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2821 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2881 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2941 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3001 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3061 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     3121 cctatctcag cgatctgcct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3181 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3241 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     3301 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3361 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     3421 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     3481 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3541 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     3601 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     3661 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     3721 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3781 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     3841 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3901 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     3961 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4021 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4081 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     4141 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     4201 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     4261 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     4321 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     4381 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     4441 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     4501 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     4561 tgcgcaactg ttgggaaggg cgatcggtgc gggcctct
//
