LOCUS       sequence-241933-        8021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multicolor Worms for Neuronal ID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8021)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS1-tax-4P::his-24::mOrange
FEATURES             Location/Qualifiers
     source          1..8021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     76..98
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     90..107
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     91..107
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     117..136
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        117..135
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3848..4558
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5324..5344)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5324..5342)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5363..5379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5363..5379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5376..5398)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5387..5403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5411..5417,5418..5435,5436..5441))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5456..5477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5594..5611)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5765..6353)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5845..5864)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6524..7315,7316..7384))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7147..7166
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7385..7489)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7515..7970)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7652..7673)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7864..7883
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
       61 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
      121 acgactcact atagggcgaa ttggagctcc accgcggact agtggatccc ccgggctgca
      181 gctcagccct ccgttcatct tttctttgcc cttctgattg atgattaatg gtacggaaaa
      241 attggatgag atttgaggag attgcggcga aatctcccaa ataccgccac tggcgagaaa
      301 aaagtagtac aaatgggtgg tcaggcggaa atgatatggt tggaagatcg gtacaaaatt
      361 ttgttgaaag tttctttaaa tctgcaatta aaagttattt gaatgaaata taaaagagtc
      421 agtttttttc agaagtcttc tcggaacttt tctaaagcac acagttctaa aacatgtaaa
      481 ttcgaaaaaa aaaccctaaa acaataatgg acgtccggtt tttccgaaag gaaaagtcgt
      541 ttcgatgaat ggaaaaccca tctttgagta gtttcagcgt gttggatata tgttgaaaaa
      601 tgctcttatg gcactttggt gtagtagttt tattccttaa atgtttcaat gaaatattaa
      661 ttagaataac gagaatattt gagactcagc cgttagacgt gttcatctta aaagtaagaa
      721 agattttgat atgaatcaga aatcttgagt ccattaaaaa actcgaaaac tgctgttatt
      781 ttgtttaaag gtttcatatt ttttccaatg agtgcatttc ttattcacaa atattttttt
      841 ttcaaaatca atgcacattc aaccttctta ggatgtttta taatccccgc ttaatgacat
      901 gagagctttg aaaatatgtg aaatgacgac caaaaagctc aggtgtacgc tcttggaaaa
      961 aaaatttctt tcgaaagtga gaactgataa tacctattta tcattgatac aagcttatcc
     1021 gacagaataa attcacttct cttgtttttg gcttcttgta gcttatttgc atatttcagt
     1081 ttgttttttt taaattattt tttgaaaaat taaattgtca ctgttctgac cggtttttta
     1141 aaatttaaaa tttgatatca gagtactcat aatcagatca agaacccata catgcctgag
     1201 ttgggatttt gcgtgcaaaa gccgtaaaag atggtcatgc tatgtgagtt tttctaattc
     1261 cattttcttt ttgttgatgt tttgatgtca ttttaaacat ggactgaatg tattttgaaa
     1321 ttgtaatagt ttttgattgg ttagttgaaa aacttttttt gtttcgaaaa tttgatcaaa
     1381 aaccggctca aatatcagag tgcagatcgg cgcacgttcg atttttaggc agtttttaac
     1441 tttaactttc taaaactttt taaatttttc tatccaaagt gaaattattt tcagaaagta
     1501 caagcatatt tctatatgtc atataaagtt actgaaagaa attttgacac aaaatggaga
     1561 aaagtacaat tctggaaatg tgtcaaaaaa tggtttcgca caaatctgtt ggattccata
     1621 tttcaaagat ggcgattttg aaggactttt tcaaaaatgt tggtcttcct ttcagaaact
     1681 ttatatagac atatagaaaa atgcttgtac tttctgaaaa taatttcact tcggatcgaa
     1741 aatttcaaaa agttactgaa aaactgtttc gaaatcgaaa tgtgacaagc gcagatatgc
     1801 actttgatat ttgaaccggt ttttgagcaa aattttgaaa ctttccattc aaagtgtagg
     1861 accaatttct aaaaaatacc tattggaaaa agcaacagaa aatagatata atttcgttat
     1921 aaactttttt atttgtttta tctacaaaaa ttgatattta ctcataaatg atcatagtgc
     1981 atatttcgta aaagattaaa ctgaaaatga actcttaaat ctattgtaaa tactcgcttg
     2041 ttcttctcgt agttcatcca ccctcgcatc tgttacttct tgcttgtcta tctcttaaca
     2101 catcctctca tatatcgagt catcattata tctccccggg caacgagctg atataactga
     2161 tactcattgc ttcttctctt tttcactctc tctcttcctc atacacctgt gcatctgcct
     2221 ctctttgcgt gtcatccctc cttttccgcc tatgctcaat ttgcttctcc agacagggat
     2281 acttttaacg acgaaaagtt cgtccttttc ctgcacatta tacgtcattt agttatttag
     2341 tttcgtgcaa ctttaacgtt tatcatttta tctttataat actcgtactg ttagttctgg
     2401 ctaacactta tagctcgtca gacgaacgtt cataattttg ttcttttctt ttgctaaatc
     2461 actgatatca catttgagct atgacaaaca tttatctcaa attaaaaaaa gagcttggcc
     2521 caaaaaagtc aaagtttgct ccataatccg gtatctatcc tccgccctca ctttccccaa
     2581 tactttccca atgaatttcc ggtttctaaa tccttttaaa gcttactttc agattctcca
     2641 aaaaattggt cacaattttt accttttatt taacttttta gtcgccatga tcgtcattat
     2701 aatatagaat aattcagata atgtattttg tttatcagta gtgcatttca ttactttact
     2761 ttggcatttt ttattttggt agagatttta agttgacaac gctttttgct cactttttct
     2821 gtttggatct tgttggtctt ttcggaaaaa aaagaagttt tacttaaagt aacgttttac
     2881 tcattctaat agtaagctta atgtcattta tttttaagct ttgtggttca ggctgctcag
     2941 ctttttcgtc atttgaactt aacttcttaa acttaacttg aaaaaaaaca aagagcaaaa
     3001 aatgctgtca attttgaaag tcgaaagaca atcgaaatat gagctttcaa gatttagctg
     3061 gaaaattaga acttaaaaat caaaaacatt gtagaattaa tatattgaat gatcgcagtg
     3121 ttcttgaatt tgtcaaattc cgagtttttg atcccttcta tcattctcaa aatttaatta
     3181 tgtttcaaga cctaggatgt ctgattccgc tgttgttgcc gccgctgtcg agccaaaggt
     3241 cccaaaggct aaggccgcca aggccgccaa gccagccgcc aaggccgcca agccaaccaa
     3301 agttgccaag gccaaggctc cagtcgctca cccaccatac atcaacatga tcaaggaggc
     3361 catcaagcag ctcaaggacc gcaaaggagc ttccaagcag gcaatcctca agttcatctc
     3421 ccaaaactac aagctcggag acaatgtcat ccagatcaat gctcatctcc gtcaggctct
     3481 caagcgtggt gtcaccagca aggcccttgt tcaagccgcc ggatccggag ccaacggacg
     3541 tttccgtgtg ccagagaagg ccgccgccgc caagaagcca gcagcagcca agaagccagc
     3601 agcagccaag aaaccagcag ccgccaagaa agccactgga gagaagaagg caaagaagcc
     3661 agccgctgcc aagccaaaga aggccgccac cggagataag aaggtcaaga aggccaagag
     3721 cccaaagaag gttgccaagc cagctgccaa gaaggtcgcc aagtctccag ccaagaaggc
     3781 cgctccaaag aagatcgcca agccagctgc caagaaggcc gccaagccag ccgccaaggc
     3841 cgcatgcatg gtgagcaagg gcgaggagaa taacatggcc atcatcaagg agttcatgcg
     3901 cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     3961 cgagggccgc ccctacgagg gctttcagac cgctaagctg aaggtgacca agggtggccc
     4021 cctgcccttc gcctgggaca tcctgtcccc tcatttcacc tacggctcca aggcctacgt
     4081 gaagcacccc gccgacatcc ccgactactt caagctgtcc ttccccgagg gcttcaagtg
     4141 ggagcgcgtg atgaactacg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     4201 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     4261 ccccgtgatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     4321 ggacggtgcc ctgaagggca agatcaagat gaggctgaag ctgaaggacg gcggccacta
     4381 cacctccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     4441 catcgtcgac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     4501 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaaa
     4561 gctttggaat tcatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat
     4621 ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt
     4681 ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag
     4741 attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca
     4801 ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt
     4861 acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat
     4921 atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg
     4981 acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag
     5041 tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt
     5101 caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca
     5161 agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa
     5221 tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat
     5281 ataccaaaca taactgtttg ggcccggtac ccagcttttg ttccctttag tgagggttaa
     5341 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5401 caattccaca caacatacga gccgggagca taaagtgtaa agcctggggt gcctaatgag
     5461 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5521 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     5581 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5641 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5701 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5761 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5821 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5881 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5941 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6001 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6061 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6121 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6181 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6241 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6301 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6361 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6421 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6481 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6541 gtgaggcacc tatctcagcg atctgcctat ttcgttcatc catagttgcc tgactccccg
     6601 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6661 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6721 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6781 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6841 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6901 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6961 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7021 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7081 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7141 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7201 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7261 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7321 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7381 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7441 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7501 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     7561 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     7621 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     7681 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     7741 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     7801 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     7861 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     7921 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     7981 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg g
//
