LOCUS       sequence-241934-       12556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of KDR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-KDR
FEATURES             Location/Qualifiers
     source          1..12556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7517..7541)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7549..7567
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             7690..7731
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7752..8319
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7769..8321
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7913..7930)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8140..8159
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8333..8932
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8333..8352)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8829..8849
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8946..9534
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8999..9019)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9417..9428)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9609..9842
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9961..9979)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10047..10151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10152..10220,10221..11012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10370..10389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11183..11771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11672..11691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11925..11942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12182..12203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12218..12223,12224..12241,12242..12248)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12256..12272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12261..12283
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12280..12296
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12280..12296
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12346..12365
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcaccatg cagagcaagg tgctgctggc cgtcgccctg
     3481 tggctctgcg tggagacccg ggccgcctct gtgggtttgc ctagtgtttc tcttgatctg
     3541 cccaggctca gcatacaaaa agacatactt acaattaagg ctaatacaac tcttcaaatt
     3601 acttgcaggg gacagaggga cttggactgg ctttggccca ataatcagag tggcagtgag
     3661 caaagggtgg aggtgactga gtgcagcgat ggcctcttct gtaagacact cacaattcca
     3721 aaagtgatcg gaaatgacac tggagcctac aagtgcttct accgggaaac tgacttggcc
     3781 tcggtcattt atgtctatgt tcaagattac agatctccat ttattgcttc tgttagtgac
     3841 caacatggag tcgtgtacat tactgagaac aaaaacaaaa ctgtggtgat tccatgtctc
     3901 gggtccattt caaatctcaa cgtgtcactt tgtgcaagat acccagaaaa gagatttgtt
     3961 cctgatggta acagaatttc ctgggacagc aagaagggct ttactattcc cagctacatg
     4021 atcagctatg ctggcatggt cttctgtgaa gcaaaaatta atgatgaaag ttaccagtct
     4081 attatgtaca tagttgtcgt tgtagggtat aggatttatg atgtggttct gagtccgtct
     4141 catggaattg aactatctgt tggagaaaag cttgtcttaa attgtacagc aagaactgaa
     4201 ctaaatgtgg ggattgactt caactgggaa tacccttctt cgaagcatca gcataagaaa
     4261 cttgtaaacc gagacctaaa aacccagtct gggagtgaga tgaagaaatt tttgagcacc
     4321 ttaactatag atggtataac ccggagtgac caaggattgt acacctgtgc agcatccagt
     4381 gggctgatga ccaagaagaa cagcacattt gtcagggtcc atgaaaaacc ttttgttgct
     4441 tttggaagtg gcatggaatc tctggtggaa gccacggtgg gggagcgtgt cagaatccct
     4501 gcgaagtacc ttggttaccc acccccagaa ataaaatggt ataaaaatgg aatacccctt
     4561 gagtccaatc acacaattaa agcggggcat gtactgacga ttatggaagt gagtgaaaga
     4621 gacacaggaa attacactgt catccttacc aatcccattt caaaggagaa gcagagccat
     4681 gtggtctctc tggttgtgta tgtcccaccc cagattggtg agaaatctct aatctctcct
     4741 gtggattcct accagtacgg caccactcaa acgctgacat gtacggtcta tgccattcct
     4801 cccccgcatc acatccactg gtattggcag ttggaggaag agtgcgccaa cgagcccagc
     4861 caagctgtct cagtgacaaa cccataccct tgtgaagaat ggagaagtgt ggaggacttc
     4921 cagggaggaa ataaaattga agttaataaa aatcaatttg ctctaattga aggaaaaaac
     4981 aaaactgtaa gtacccttgt tatccaagcg gcaaatgtgt cagctttgta caaatgtgaa
     5041 gcggtcaaca aagtcgggag aggagagagg gtgatctcct tccacgtgac caggggtcct
     5101 gaaattactt tgcaacctga catgcagccc actgagcagg agagcgtgtc tttgtggtgc
     5161 actgcagaca gatctacgtt tgagaacctc acatggtaca agcttggccc acagcctctg
     5221 ccaatccatg tgggagagtt gcccacacct gtttgcaaga acttggatac tctttggaaa
     5281 ttgaatgcca ccatgttctc taatagcaca aatgacattt tgatcatgga gcttaagaat
     5341 gcatccttgc aggaccaagg agactatgtc tgccttgctc aagacaggaa gaccaagaaa
     5401 agacattgcg tggtcaggca gctcacagtc ctagagcgtg tggcacccac gatcacagga
     5461 aacctggaga atcagacgac aagtattggg gaaagcatcg aagtctcatg cacggcatct
     5521 gggaatcccc ctccacagat catgtggttt aaagataatg agacccttgt agaagactca
     5581 ggcattgtat tgaaggatgg gaaccggaac ctcactatcc gcagagtgag gaaggaggac
     5641 gaaggcctct acacctgcca ggcatgcagt gttcttggct gtgcaaaagt ggaggcattt
     5701 ttcataatag aaggtgccca ggaaaagacg aacttggaaa tcattattct agtaggcacg
     5761 gcggtgattg ccatgttctt ctggctactt cttgtcatca tcctacggac cgttaagcgg
     5821 gccaatggag gggaactgaa gacaggctac ttgtccatcg tcatggatcc agatgaactc
     5881 ccattggatg aacattgtga acgactgcct tatgatgcca gcaaatggga attccccaga
     5941 gaccggctga agctaggtaa gcctcttggc cgtggtgcct ttggccaagt gattgaagca
     6001 gatgcctttg gaattgacaa gacagcaact tgcaggacag tagcagtcaa aatgttgaaa
     6061 gaaggagcaa cacacagtga gcatcgagct ctcatgtctg aactcaagat cctcattcat
     6121 attggtcacc atctcaatgt ggtcaacctt ctaggtgcct gtaccaagcc aggagggcca
     6181 ctcatggtga ttgtggaatt ctgcaaattt ggaaacctgt ccacttacct gaggagcaag
     6241 agaaatgaat ttgtccccta caagaccaaa ggggcacgat tccgtcaagg gaaagactac
     6301 gttggagcaa tccctgtgga tctgaaacgg cgcttggaca gcatcaccag tagccagagc
     6361 tcagccagct ctggatttgt ggaggagaag tccctcagtg atgtagaaga agaggaagct
     6421 cctgaagatc tgtataagga cttcctgacc ttggagcatc tcatctgtta cagcttccaa
     6481 gtggctaagg gcatggagtt cttggcatcg cgaaagtgta tccacaggga cctggcggca
     6541 cgaaatatcc tcttatcgga gaagaacgtg gttaaaatct gtgactttgg cttggcccgg
     6601 gatatttata aagatccaga ttatgtcaga aaaggagatg ctcgcctccc tttgaaatgg
     6661 atggccccag aaacaatttt tgacagagtg tacacaatcc agagtgacgt ctggtctttt
     6721 ggtgttttgc tgtgggaaat attttcctta ggtgcttctc catatcctgg ggtaaagatt
     6781 gatgaagaat tttgtaggcg attgaaagaa ggaactagaa tgagggcccc tgattatact
     6841 acaccagaaa tgtaccagac catgctggac tgctggcacg gggagcccag tcagagaccc
     6901 acgttttcag agttggtgga acatttggga aatctcttgc aagctaatgc tcagcaggat
     6961 ggcaaagact acattgttct tccgatatca gagactttga gcatggaaga ggattctgga
     7021 ctctctctgc ctacctcacc tgtttcctgt atggaggagg aggaagtatg tgaccccaaa
     7081 ttccattatg acaacacagc aggaatcagt cagtatctgc agaacagtaa gcgaaagagc
     7141 cggcctgtga gtgtaaaaac atttgaagat atcccgttag aagaaccaga agtaaaagta
     7201 atcccagatg acaaccagac ggacagtggt atggttcttg cctcagaaga gctgaaaact
     7261 ttggaagaca gaaccaaatt atctccatct tttggtggaa tggtgcccag caaaagcagg
     7321 gagtctgtgg catctgaagg ctcaaaccag acaagcggct accagtccgg atatcactcc
     7381 gatgacacag acaccaccgt gtactccagt gaggaagcag aacttttaaa gctgatagag
     7441 attggagtgc aaaccggtag cacagcccag attctccagc ctgactcggg gaccacactg
     7501 agctctcctc ctgttttgcc aactttcttg tacaaagtgg tgatatccaa ttaaccctca
     7561 ctaaaggatg aaagttagag tttatgataa gccctatagt gatcgtatta cacccaactt
     7621 ttctaataca aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg
     7681 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
     7741 tagtaatgag atccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     7801 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     7861 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     7921 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     7981 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     8041 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     8101 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     8161 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     8221 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     8281 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac atatgaccga
     8341 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     8401 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     8461 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     8521 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     8581 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     8641 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     8701 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     8761 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     8821 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     8881 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct aaatcgatag
     8941 atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     9001 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     9061 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     9121 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     9181 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     9241 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     9301 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     9361 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     9421 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     9481 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga
     9541 tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     9601 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     9661 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     9721 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     9781 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     9841 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     9901 agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg
     9961 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
    10021 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    10081 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    10141 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    10201 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    10261 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    10321 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    10381 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    10441 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10501 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10561 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10621 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10681 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10741 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10801 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10861 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10921 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10981 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    11041 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    11101 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    11161 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    11221 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    11281 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    11341 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    11401 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    11461 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11521 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    11581 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11641 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11701 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11761 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11821 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11881 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11941 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    12001 aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    12061 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    12121 aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    12181 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    12241 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
    12301 ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    12361 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    12421 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    12481 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    12541 ctacaaactt agtagt
//
