LOCUS       sequence-241938-        9489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Localized Pan-Neuronal tagBFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Sharma AK, Randi F, Kumar S, Dvali S, Leifer AM
  TITLE     TWISP: a transgenic worm for interrogating signal propagation in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2024 Jul 8;227(3):iyae077. doi: 10.1093/genetics/iyae077.
  PUBMED    38733622
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2024-07-8"; volume: "227(3):iyae077. doi"; pages: " 
            10.1093/genetics/iyae077"
COMMENT     Sequence Label: pAS1-rab-3P::his-24::tagBFP
FEATURES             Location/Qualifiers
     source          1..9489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     192..211
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        192..210
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6867..6887)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6867..6885)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6906..6922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6906..6922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6919..6941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6930..6946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6954..6960,6961..6978,6979..6984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6999..7020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7137..7154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7308..7896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7388..7407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8067..8858,8859..8927))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8690..8709
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8928..9032)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(9058..9489,1..24))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9195..9216)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9407..9426
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgagcgcgc gtaatacgac tcactatagg gcgaattgga gctccaccgc ggactagtgg
      241 atcccccggg ctgcaggcga gttttgactg gctttcaaaa actgagtgat tactaattat
      301 ttttgaaaaa attaacagtt gtgatatgat atttagacat ttttgcaata tataatttgt
      361 ttgaaaaaaa aacatttttt aatggtcttc gaagctataa tagtttttcg taaaatttta
      421 aataaaacag ctgacagata tccacattca ataattgaaa gtatgcagtt ctcttttttt
      481 ttgtcaaata gaaaaaaagt aacagatggt cagatataat ctaggttaat ctgactctgg
      541 tcatcgatga actcattttc atcataatca ttcttctttc aaatggtctg ttcggaattc
      601 gaatattcaa taaatggata tcttattaga tgctccggat ttattccacc aaacttccaa
      661 gacgtaagtt caatttaaac aaaattacta tagcttgaaa aaatgagaaa aaacgagcag
      721 ataaaaaaat aacagaaaaa atccataaaa atgacgaggg ttccgtgcga aaatcgtcat
      781 tgtttttggc ttgcatcaca cttttccgcg agtcagccac actgttttat cgattattat
      841 ttttctgatt tcagaaaccg gaaacttaca actgagatgc tttcattttg atttttttaa
      901 atttagtttt gatttcagcg atttgtttga atataattat gcatgagttg gcttttgcat
      961 ttttagataa ataagttcac atgctcggac aaattgtgtg gtttaatctg tgaaagtttt
     1021 gaatcttctg tttgaaaaaa aaaacatttt taatttgtat atacgagtga attggttagg
     1081 ccctggttac gttttccagc gaattcattc aaatttctta ttttttctat tttttcaatt
     1141 aaataattta gttctctatc acatgtgtat gatcaaaatt tcattcctaa tcgtaagcaa
     1201 atttaggctc atccgtagta taattttgtt atgaagggct tcacagtaaa agctcaaact
     1261 ccaacattta gcaactagca accaacaaaa aaccaaagaa atgtcagttt tgaaaagtcg
     1321 ttatttcttt gtcttctcac aattcacaca tcatcttctc gttttcctcc gtttttttgt
     1381 cgtgcttcct tcttttcttt acacattttt ctaacctttt tttaagtcta tcctctccta
     1441 atcctttgta ctctctaatc agttttgctg gttgtgctgc acaattttta tcagatttat
     1501 gcgcgaaaca aaagcctctc gcggagagct ccgccaaatc cggtcttctt ttttccttgc
     1561 ctatcggatt tcacaaacat tcagtttgtc acttgacgat gtgttggagg cattactact
     1621 aggaagtgtt attcatattt tttattcgtt tctttttggt gactaaacga aatattccga
     1681 acgttttcct gatttcaggc ggcggaaacg aagaagacca cgaataatca acaggctgcc
     1741 attgcatccg ccagaagtcg agtgagttct ttgaaaatct ttattagagt tatttgtgac
     1801 aatttgccac ttttttttta atgtttaatg aatctgtaac gaaattaata ccaagggtgt
     1861 ccaacagaac tatctgagcg gttatttaat ttttagatag tatatttctt ccgatcgtaa
     1921 catacatatt ttcaagcata gtactgtgat ttaagttaca gttttccaat cagtcttgat
     1981 tttaataaaa acttttcaac tgcaaaaatg cctctgaagt atttttctat atttttatag
     2041 ttgaataatt ttttgattgg cacaaagacc gccgagatat gagccgttaa agtaaagctg
     2101 catggattca atatggtttg tatttgattg gagtcactta catggaaata tttgtattta
     2161 aatctcatat tttaaaacaa gacaaacacc aaattaaatt tttattaatc tgaacatttt
     2221 tcaatcgatt tggtaaacaa attattagat gtttccctca aaaattgtaa caattcctaa
     2281 attgtggcac ttataataac atcgtcctga attctcaaaa aaaaccgaat gctaagagag
     2341 atttcgttta actgggacct gtaactgaat tttcaaatat tggcagtttt tgataaactg
     2401 ggagggaaat caaaaaccaa aactacagta cccctcgtgt tataataaat tgtcattcac
     2461 tccttttttg ttcccaacat ttgctcccat ctcctgacga gaaagaacaa ttttatgagc
     2521 acgggaaccc gtgggcgtcc atccgttcct cctgtgcaat ttcagccccc ttcctctaca
     2581 ttctctgtgt tcccgttttt atcgttttat gggtatcggg aacggacaga tcgcttccat
     2641 ttgcttctat tccgtcctca caagacgtcc cactaactga ttttcttctg atttcttttc
     2701 actgaaaatg ggaaagaaag aaaaagacga tgaatcgacg acttcttctc aggagaaggt
     2761 gaagaagaag gataaggaga aagttcatct aaggctgaaa gataaagtaa aaaaacttag
     2821 aaaatctaag tcgattgcag tatcttctcc agctgattat atgacttgtc agtgtgaacc
     2881 atgcaaaaaa gtagctctcg aggtaataat ttttaaactc aatacgtagt taagtttgaa
     2941 ctccttttaa tttacttatc atctaattta ccgtcacaat agtacttcca ttagcaatta
     3001 ttttaaaatt acatgcatag cttcaatgtt cgtttttttg cgttctcata tcgtatgttg
     3061 tctgcctctc gaatactgac tgtccgacta gaatgtcccg gtaggattac tgtacggtca
     3121 ctgggaataa actgtttctg aaatcaatct gaaattccgt agatcttctt ggttcaattt
     3181 cttattcctc aatctttgga caacgtcacc ttcagagtcg tcgttgagta tcccatcatt
     3241 aactagtcaa tctttctctc tttttcattt cccccaactt ttctgcatat tttttgacga
     3301 cgacgacctc gacggcaacg agaatacatt tatctatttt ttagtatagc atatatacat
     3361 tttctacttt tctctaaccc atttcagacg tatagatttt ggttttcgaa tgcaactttg
     3421 agatttttca aagatcttca gatgggagca gtggactgtc tatttgatat tcttaaaatg
     3481 tactctataa aagatgaaac tgtaatgacc aattccctac ataaatattt ttaactaatt
     3541 ggtcggatat ttggggatca gagaagtact tgtttttgcg atattaataa tgatatattt
     3601 ttctagtaaa aaaatgttaa gcgatttgtg aataattaag ttggcttgaa aaagattttt
     3661 tgatagctat gtattttgtt aatttaaatc agattttaaa actttattat tgactattta
     3721 tatatattat atattatata ttttcatggt ttaatcaaaa tctattaaat tatttctaag
     3781 atctctaaat atcgaattct ctaaaaagct tcatcaacaa aagcatttca attatctaac
     3841 acaatgagaa taattcaaga actgaaatag aaagaaatct agaaaaaaaa gagagtgagg
     3901 tgccctatta aatcggtgct ctcctctcta acagaagcgc ctacacgcac ccaagagagc
     3961 tctttttctt tcttttttcg gagcacacat acacgcatac actcctcaga agtgtataga
     4021 gaaaatggga gaagaagcga aagaagacga agaagcggtt gtgatgtatg catcgggcgt
     4081 ttctatccct ctccgtgagc aacgagctag tcaacccatc gatccatttt tgtgagcaca
     4141 cacagagaga gactcaaaca cactcacagc ttcacactga gctccaagag ctctgtagag
     4201 agaagccaga gtatgctttc ctcgattttc ctaacgtctt cttcttctgc ttttttcttt
     4261 ttcccaacga gttgtagtcc actaacttca atttttcctc cagagaaaga gttttagaga
     4321 gacagctgat aaatacacta gagaaataaa aagaatgata aaaatttgct attttaatca
     4381 gttccctctc gtttctctaa gttttgttgt tgggttggga aattaaaatt ggaaaatgga
     4441 aataacgttt ttttctgtct aatcttgaat tctccagaca tttcttattt ccttttttgt
     4501 tgcaagtttt tcttccttct ctaattttta atcaaagata tctcgctatg aactttgaat
     4561 ttttgatatt ttgattctat aaatacataa agtaatctaa atgctctttt aaaataaatc
     4621 tacagtagcc ctattttcag cctaggatgt ctgattccgc tgttgttgcc gccgctgtcg
     4681 agccaaaggt cccaaaggct aaggccgcca aggccgccaa gccagccgcc aaggccgcca
     4741 agccaaccaa agttgccaag gccaaggctc cagtcgctca cccaccatac atcaacatga
     4801 tcaaggaggc catcaagcag ctcaaggacc gcaaaggagc ttccaagcag gcaatcctca
     4861 agttcatctc ccaaaactac aagctcggag acaatgtcat ccagatcaat gctcatctcc
     4921 gtcaggctct caagcgtggt gtcaccagca aggcccttgt tcaagccgcc ggatccggag
     4981 ccaacggacg tttccgtgtg ccagagaagg ccgccgccgc caagaagcca gcagcagcca
     5041 agaagccagc agcagccaag aaaccagcag ccgccaagaa agccactgga gagaagaagg
     5101 caaagaagcc agccgctgcc aagccaaaga aggccgccac cggagataag aaggtcaaga
     5161 aggccaagag cccaaagaag gttgccaagc cagctgccaa gaaggtcgcc aagtctccag
     5221 ccaagaaggc cgctccaaag aagatcgcca agccagctgc caagaaggcc gccaagccag
     5281 ccgccaaggc cgcatgcatg tccgaactca tcaaggagaa catgcacatg aagctctaca
     5341 tggagggaac cgtagacaac caccacttca agtgcacctc cgagggagag ggaaagccat
     5401 acgagggaac ccaaaccatg cgtatcaagg tcgtcgaggg aggaccactc ccattcgcct
     5461 tcgacatcct cgccacctcc ttcctctacg ggtccaaggt aagtttaaac atatatatac
     5521 taactaaccc tgattattta aattttcaga ccttcatcaa ccacacccaa ggaatcccag
     5581 acttcttcaa gcaatccttc ccagagggat tcacctggga gcgtgtcacc acctacgagg
     5641 acggaggagt cctcaccgcc acccaagaca cctccctcca agacggatgc ctcatctaca
     5701 acgtcaagat ccgtggagtc aacttcacct ccaacggacc agtcatgcaa aagaagaccc
     5761 tcggatggga gaccttcacc gagaccctct acccagccga cggaggacta gagggacgta
     5821 acgacatggc cctcaagctc gtcggaggct cccacctcat cgccaacatc aaggtaagtt
     5881 taaacagttc ggtactaact aaccatacat atttaaattt tcagaccacc taccgttcca
     5941 agaagccagc caagaacctc aagatgccag gagtctacta cgttgactac cgtctagagc
     6001 gtatcaagga ggccaacaac gagacctacg tcgagcaaca cgaggtcgcc gtcgcccgtt
     6061 actgcgacct cccatccaag ctcggacaca agctcaacta aaagctttgg aattcatctc
     6121 gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct
     6181 ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt
     6241 ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta
     6301 attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc
     6361 ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt
     6421 ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt
     6481 atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca
     6541 tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt
     6601 aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg
     6661 cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag
     6721 gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt
     6781 gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt
     6841 ttgggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     6901 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6961 cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7021 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7081 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7141 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7201 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7261 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7321 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7381 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7441 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7501 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7561 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7621 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7681 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7741 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7801 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7861 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7921 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7981 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8041 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8101 gcgatctgcc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8161 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8221 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8281 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8341 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8401 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8461 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8521 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8581 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8641 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8701 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8761 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8821 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8881 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8941 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9001 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     9061 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     9121 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     9181 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     9241 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     9301 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     9361 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     9421 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     9481 ccgccgcgc
//
