LOCUS       sequence-241940-        6662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Wild-type Lamin A, C-terminally tagged with mRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6662)
  TITLE     Schirmer lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label:  Lamin A-mRFP
FEATURES             Location/Qualifiers
     source          1..6662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    663..719
                     /label=MCS
                     /note="multiple cloning site"
     CDS             2696..3370
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVIQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2826..2844)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3288..3311
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3504..3625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3541..3560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3595..3614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3632..4087)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3769..3790)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3981..4000
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4114..4218
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4220..4577
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4220..4411
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4243..4263)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4428..4563
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4612..5406
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4612..5406
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4666..4685)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5276..5295
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5594..5613)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5638..5685
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6014..6602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6503..6522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac
      661 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatccg
      721 aatggccatg gagaccccgt cccagcggcg cgccacccgc agcggggcgc aggccagctc
      781 cactccgctg tcgcccaccc gcatcacccg gctgcaggag aaggaggacc tgcaggagct
      841 caatgatcgc ttggcggtct acatcgaccg tgtgcgctcg ctggaaacgg agaacgcagg
      901 gctgcgcctt cgcatcaccg agtctgaaga ggtggtcagc cgcgaggtgt ccggcatcaa
      961 ggccgcctac gaggccgagc tcggggatgc ccgcaagacc cttgactcag tagccaagga
     1021 gcgcgcccgc ctgcagctgg agctgagcaa agtgcgtgag gagtttaagg agctgaaagc
     1081 gcgcaatacc aagaaggagg gtgacctgat agctgctcag gctcggctga aggacctgga
     1141 ggctctgctg aactccaagg aggccgcact gagcactgct ctcagtgaga agcgcacgct
     1201 ggagggcgag ctgcatgatc tgcggggcca ggtggccaag cttgaggcag ccctaggtga
     1261 ggccaagaag caacttcagg atgagatgct gcggcgggtg gatgctgaga acaggctgca
     1321 gaccatgaag gaggaactgg acttccagaa gaacatctac agtgaggagc tgcgtgagac
     1381 caagcgccgt catgagaccc gactggtgga gattgacaat gggaagcagc gtgagtttga
     1441 gagccggctg gcggatgcgc tgcaggaact gcgggcccag catgaggacc aggtggagca
     1501 gtataagaag gagctggaga agacttattc tgccaagctg gacaatgcca ggcagtctgc
     1561 tgagaggaac agcaacctgg tgggggctgc ccacgaggag ctgcagcagt cgcgcatccg
     1621 catcgacagc ctctctgccc agctcagcca gctccagaag cagctggcag ccaaggaggc
     1681 gaagcttcga gacctggagg actcactggc ccgtgagcgg gacaccagcc ggcggctgct
     1741 ggcggaaaag gagcgggaga tggccgagat gcgggcaagg atgcagcagc agctggacga
     1801 gtaccaggag cttctggaca tcaagctggc cctggacatg gagatccacg cctaccgcaa
     1861 gctcttggag ggcgaggagg agaggctacg cctgtccccc agccctacct cgcagcgcag
     1921 ccgtggccgt gcttcctctc actcatccca gacacagggt gggggcagcg tcaccaaaaa
     1981 gcgcaaactg gagtccactg agagccgcag cagcttctca cagcacgcac gcactagcgg
     2041 gcgcgtggcc gtggaggagg tggatgagga gggcaagttt gtccggctgc gcaacaagtc
     2101 caatgaggac cagtccatgg gcaattggca gatcaagcgc cagaatggag atgatccctt
     2161 gctgacttac cggttcccac caaagttcac cctgaaggct gggcaggtgg tgacgatctg
     2221 ggctgcagga gctggggcca cccacagccc ccctaccgac ctggtgtgga aggcacagaa
     2281 cacctggggc tgcgggaaca gcctgcgtac ggctctcatc aactccactg gggaagaagt
     2341 ggccatgcgc aagctggtgc gctcagtgac tgtggttgag gacgacgagg atgaggatgg
     2401 agatgacctg ctccatcacc accacggctc ccactgcagc agctcggggg accccgctga
     2461 gtacaacctg cgctcgcgca ccgtgctgtg cgggacctgc gggcagcctg ccgacaaggc
     2521 atctgccagc ggctcaggag cccaggtggg cggacccatc tcctctggct cttctgcctc
     2581 cagtgtcacg gtcactcgca gctaccgcag tgtggggggc agtgggggtg gcagcttcgg
     2641 ggacaatctg gtcacccgct cctacggtgg cggtggctct ggaggtggtg ggtccatggc
     2701 ctcctccgag gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt
     2761 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     2821 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     2881 ccctcagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     2941 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3001 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     3061 gaagctgcgc ggcaccaact tcccctccga cggccccgta attcagaaga agacgatggg
     3121 ctgggaggcc tccaccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     3181 gatgaggctg aagctgaagg acggcggcca ctacgacgcc gaggtcaaga ccacctacat
     3241 ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc tggacatcac
     3301 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg gccgccactc
     3361 caccggcgcc ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat
     3421 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     3481 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3541 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3601 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa
     3661 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     3721 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     3781 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     3841 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     3901 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     3961 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     4021 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     4081 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4141 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4201 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     4261 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4321 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4381 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4441 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4501 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4561 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     4621 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4681 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     4741 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     4801 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     4861 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4921 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     4981 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5041 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5101 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     5161 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5221 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5281 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5341 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5401 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5461 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5521 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     5581 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     5641 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     5701 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     5761 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     5821 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     5881 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5941 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6001 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6061 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6121 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6661 tg
//
