LOCUS       sequence-241941-        9489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Localized Pan-Neuronal tagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9489)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS1-rab-3P::his24::tagBFP2
FEATURES             Location/Qualifiers
     source          1..9489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     70..92
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     84..101
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     85..101
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     111..130
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        111..129
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6786..6806)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6786..6804)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6825..6841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6825..6841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6838..6860)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6849..6865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6873..6879,6880..6897,6898..6903))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6918..6939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7056..7073)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7227..7815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7307..7326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7986..8777,8778..8846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8609..8628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8847..8951)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8977..9432)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9114..9135)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9326..9345
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
       61 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
      121 cactataggg cgaattggag ctccaccgcg gactagtgga tcccccgggc tgcaggcgag
      181 ttttgactgg ctttcaaaaa ctgagtgatt actaattatt tttgaaaaaa ttaacagttg
      241 tgatatgata tttagacatt tttgcaatat ataatttgtt tgaaaaaaaa acatttttta
      301 atggtcttcg aagctataat agtttttcgt aaaattttaa ataaaacagc tgacagatat
      361 ccacattcaa taattgaaag tatgcagttc tctttttttt tgtcaaatag aaaaaaagta
      421 acagatggtc agatataatc taggttaatc tgactctggt catcgatgaa ctcattttca
      481 tcataatcat tcttctttca aatggtctgt tcggaattcg aatattcaat aaatggatat
      541 cttattagat gctccggatt tattccacca aacttccaag acgtaagttc aatttaaaca
      601 aaattactat agcttgaaaa aatgagaaaa aacgagcaga taaaaaaata acagaaaaaa
      661 tccataaaaa tgacgagggt tccgtgcgaa aatcgtcatt gtttttggct tgcatcacac
      721 ttttccgcga gtcagccaca ctgttttatc gattattatt tttctgattt cagaaaccgg
      781 aaacttacaa ctgagatgct ttcattttga tttttttaaa tttagttttg atttcagcga
      841 tttgtttgaa tataattatg catgagttgg cttttgcatt tttagataaa taagttcaca
      901 tgctcggaca aattgtgtgg tttaatctgt gaaagttttg aatcttctgt ttgaaaaaaa
      961 aaacattttt aatttgtata tacgagtgaa ttggttaggc cctggttacg ttttccagcg
     1021 aattcattca aatttcttat tttttctatt ttttcaatta aataatttag ttctctatca
     1081 catgtgtatg atcaaaattt cattcctaat cgtaagcaaa tttaggctca tccgtagtat
     1141 aattttgtta tgaagggctt cacagtaaaa gctcaaactc caacatttag caactagcaa
     1201 ccaacaaaaa accaaagaaa tgtcagtttt gaaaagtcgt tatttctttg tcttctcaca
     1261 attcacacat catcttctcg ttttcctccg tttttttgtc gtgcttcctt cttttcttta
     1321 cacatttttc taaccttttt ttaagtctat cctctcctaa tcctttgtac tctctaatca
     1381 gttttgctgg ttgtgctgca caatttttat cagatttatg cgcgaaacaa aagcctctcg
     1441 cggagagctc cgccaaatcc ggtcttcttt tttccttgcc tatcggattt cacaaacatt
     1501 cagtttgtca cttgacgatg tgttggaggc attactacta ggaagtgtta ttcatatttt
     1561 ttattcgttt ctttttggtg actaaacgaa atattccgaa cgttttcctg atttcaggcg
     1621 gcggaaacga agaagaccac gaataatcaa caggctgcca ttgcatccgc cagaagtcga
     1681 gtgagttctt tgaaaatctt tattagagtt atttgtgaca atttgccact ttttttttaa
     1741 tgtttaatga atctgtaacg aaattaatac caagggtgtc caacagaact atctgagcgg
     1801 ttatttaatt tttagatagt atatttcttc cgatcgtaac atacatattt tcaagcatag
     1861 tactgtgatt taagttacag ttttccaatc agtcttgatt ttaataaaaa cttttcaact
     1921 gcaaaaatgc ctctgaagta tttttctata tttttatagt tgaataattt tttgattggc
     1981 acaaagaccg ccgagatatg agccgttaaa gtaaagctgc atggattcaa tatggtttgt
     2041 atttgattgg agtcacttac atggaaatat ttgtatttaa atctcatatt ttaaaacaag
     2101 acaaacacca aattaaattt ttattaatct gaacattttt caatcgattt ggtaaacaaa
     2161 ttattagatg tttccctcaa aaattgtaac aattcctaaa ttgtggcact tataataaca
     2221 tcgtcctgaa ttctcaaaaa aaaccgaatg ctaagagaga tttcgtttaa ctgggacctg
     2281 taactgaatt ttcaaatatt ggcagttttt gataaactgg gagggaaatc aaaaaccaaa
     2341 actacagtac ccctcgtgtt ataataaatt gtcattcact ccttttttgt tcccaacatt
     2401 tgctcccatc tcctgacgag aaagaacaat tttatgagca cgggaacccg tgggcgtcca
     2461 tccgttcctc ctgtgcaatt tcagccccct tcctctacat tctctgtgtt cccgttttta
     2521 tcgttttatg ggtatcggga acggacagat cgcttccatt tgcttctatt ccgtcctcac
     2581 aagacgtccc actaactgat tttcttctga tttcttttca ctgaaaatgg gaaagaaaga
     2641 aaaagacgat gaatcgacga cttcttctca ggagaaggtg aagaagaagg ataaggagaa
     2701 agttcatcta aggctgaaag ataaagtaaa aaaacttaga aaatctaagt cgattgcagt
     2761 atcttctcca gctgattata tgacttgtca gtgtgaacca tgcaaaaaag tagctctcga
     2821 ggtaataatt tttaaactca atacgtagtt aagtttgaac tccttttaat ttacttatca
     2881 tctaatttac cgtcacaata gtacttccat tagcaattat tttaaaatta catgcatagc
     2941 ttcaatgttc gtttttttgc gttctcatat cgtatgttgt ctgcctctcg aatactgact
     3001 gtccgactag aatgtcccgg taggattact gtacggtcac tgggaataaa ctgtttctga
     3061 aatcaatctg aaattccgta gatcttcttg gttcaatttc ttattcctca atctttggac
     3121 aacgtcacct tcagagtcgt cgttgagtat cccatcatta actagtcaat ctttctctct
     3181 ttttcatttc ccccaacttt tctgcatatt ttttgacgac gacgacctcg acggcaacga
     3241 gaatacattt atctattttt tagtatagca tatatacatt ttctactttt ctctaaccca
     3301 tttcagacgt atagattttg gttttcgaat gcaactttga gatttttcaa agatcttcag
     3361 atgggagcag tggactgtct atttgatatt cttaaaatgt actctataaa agatgaaact
     3421 gtaatgacca attccctaca taaatatttt taactaattg gtcggatatt tggggatcag
     3481 agaagtactt gtttttgcga tattaataat gatatatttt tctagtaaaa aaatgttaag
     3541 cgatttgtga ataattaagt tggcttgaaa aagatttttt gatagctatg tattttgtta
     3601 atttaaatca gattttaaaa ctttattatt gactatttat atatattata tattatatat
     3661 tttcatggtt taatcaaaat ctattaaatt atttctaaga tctctaaata tcgaattctc
     3721 taaaaagctt catcaacaaa agcatttcaa ttatctaaca caatgagaat aattcaagaa
     3781 ctgaaataga aagaaatcta gaaaaaaaag agagtgaggt gccctattaa atcggtgctc
     3841 tcctctctaa cagaagcgcc tacacgcacc caagagagct ctttttcttt cttttttcgg
     3901 agcacacata cacgcataca ctcctcagaa gtgtatagag aaaatgggag aagaagcgaa
     3961 agaagacgaa gaagcggttg tgatgtatgc atcgggcgtt tctatccctc tccgtgagca
     4021 acgagctagt caacccatcg atccattttt gtgagcacac acagagagag actcaaacac
     4081 actcacagct tcacactgag ctccaagagc tctgtagaga gaagccagag tatgctttcc
     4141 tcgattttcc taacgtcttc ttcttctgct tttttctttt tcccaacgag ttgtagtcca
     4201 ctaacttcaa tttttcctcc agagaaagag ttttagagag acagctgata aatacactag
     4261 agaaataaaa agaatgataa aaatttgcta ttttaatcag ttccctctcg tttctctaag
     4321 ttttgttgtt gggttgggaa attaaaattg gaaaatggaa ataacgtttt tttctgtcta
     4381 atcttgaatt ctccagacat ttcttatttc cttttttgtt gcaagttttt cttccttctc
     4441 taatttttaa tcaaagatat ctcgctatga actttgaatt tttgatattt tgattctata
     4501 aatacataaa gtaatctaaa tgctctttta aaataaatct acagtagccc tattttcagc
     4561 ctaggatgtc tgattccgct gttgttgccg ccgctgtcga gccaaaggtc ccaaaggcta
     4621 aggccgccaa ggccgccaag ccagccgcca aggccgccaa gccaaccaaa gttgccaagg
     4681 ccaaggctcc agtcgctcac ccaccataca tcaacatgat caaggaggcc atcaagcagc
     4741 tcaaggaccg caaaggagct tccaagcagg caatcctcaa gttcatctcc caaaactaca
     4801 agctcggaga caatgtcatc cagatcaatg ctcatctccg tcaggctctc aagcgtggtg
     4861 tcaccagcaa ggcccttgtt caagccgccg gatccggagc caacggacgt ttccgtgtgc
     4921 cagagaaggc cgccgccgcc aagaagccag cagcagccaa gaagccagca gcagccaaga
     4981 aaccagcagc cgccaagaaa gccactggag agaagaaggc aaagaagcca gccgctgcca
     5041 agccaaagaa ggccgccacc ggagataaga aggtcaagaa ggccaagagc ccaaagaagg
     5101 ttgccaagcc agctgccaag aaggtcgcca agtctccagc caagaaggcc gctccaaaga
     5161 agatcgccaa gccagctgcc aagaaggccg ccaagccagc cgccaaggcc gcatgcatgt
     5221 ccgaactcat caaggagaac atgcacatga agctctacat ggagggaacc gtagacaacc
     5281 accacttcaa gtgcacctcc gagggagagg gaaagccata cgagggaacc caaaccatgc
     5341 gtatcaaggt cgtcgaggga ggaccactcc cattcgcctt cgacatcctc gccacctcct
     5401 tcctctacgg gtccaaggta agtttaaaca tatatatact aactaaccct gattatttaa
     5461 attttcagac cttcatcaac cacacccaag gaatcccaga cttcttcaag caatccttcc
     5521 cagagggatt cacctgggag cgtgtcacca cctacgagga cggaggagtc ctcaccgcca
     5581 cccaagacac ctccctccaa gacggatgcc tcatctacaa cgtcaagatc cgtggagtca
     5641 acttcacctc caacggacca gtcatgcaaa agaagaccct cggatgggag accttcaccg
     5701 agaccctcta cccagccgac ggaggactag agggacgtaa cgacatggcc ctcaagctcg
     5761 tcggaggctc ccacctcatc gccaacgcca aggtaagttt aaacagttcg gtactaacta
     5821 accatacata tttaaatttt cagaccacct accgttccaa gaagccagcc aagaacctca
     5881 agatgccagg agtctactac gttgactacc gtctagagcg tatcaaggag gccaacaacg
     5941 agacctacgt cgagcaacac gaggtcgccg tcgcccgtta ctgcgacctc ccatccaagc
     6001 tcggacacaa gctcaactaa aagctttgga attcatctcg cgcccgtgcc tctgacttct
     6061 aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc caccccctat
     6121 ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct tttaagtcac
     6181 ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa
     6241 aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac acaatgttct
     6301 gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca tagaaaaaac
     6361 cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat ttttatttct
     6421 tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta
     6481 tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc ttttgctttt
     6541 tggggtttcc cctattgttt gtcaagattt cgaggacggc gtttttcttg ctaaaatcac
     6601 aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg aggctcagtg
     6661 gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt ttttgcctta
     6721 aatgacagaa tacattccca atataccaaa cataactgtt tgggcccggt acccagcttt
     6781 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     6841 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccgggag cataaagtgt
     6901 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6961 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7021 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7081 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7141 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7201 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7261 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7321 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7381 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7441 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7501 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7561 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7621 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7681 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7741 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7801 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7861 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7921 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7981 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgcct atttcgttca
     8041 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8101 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8161 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8221 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8281 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8341 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8401 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8461 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8521 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8581 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8641 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8701 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8761 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8821 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8881 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8941 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     9001 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     9061 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     9121 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     9181 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     9241 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     9301 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     9361 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     9421 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     9481 gggcctctt
//
