LOCUS       sequence-241953-        9396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Localized Pan-Neuronal mOrange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9396)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS1-rab-3P::his-24::mOrange
FEATURES             Location/Qualifiers
     source          1..9396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     88..110
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     102..119
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     103..119
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     129..148
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        129..147
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5235..5945
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6711..6731)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6711..6729)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6750..6766)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6750..6766)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6763..6785)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6774..6790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6798..6804,6805..6822,6823..6828))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6843..6864
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6981..6998)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7152..7740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7232..7251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7911..8702,8703..8771))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8534..8553
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8772..8876)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8902..9357)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9039..9060)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9251..9270
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
       61 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
      121 agcgcgcgta atacgactca ctatagggcg aattggagct ccaccgcgga ctagtggatc
      181 ccccgggctg caggcgagtt ttgactggct ttcaaaaact gagtgattac taattatttt
      241 tgaaaaaatt aacagttgtg atatgatatt tagacatttt tgcaatatat aatttgtttg
      301 aaaaaaaaac attttttaat ggtcttcgaa gctataatag tttttcgtaa aattttaaat
      361 aaaacagctg acagatatcc acattcaata attgaaagta tgcagttctc tttttttttg
      421 tcaaatagaa aaaaagtaac agatggtcag atataatcta ggttaatctg actctggtca
      481 tcgatgaact cattttcatc ataatcattc ttctttcaaa tggtctgttc ggaattcgaa
      541 tattcaataa atggatatct tattagatgc tccggattta ttccaccaaa cttccaagac
      601 gtaagttcaa tttaaacaaa attactatag cttgaaaaaa tgagaaaaaa cgagcagata
      661 aaaaaataac agaaaaaatc cataaaaatg acgagggttc cgtgcgaaaa tcgtcattgt
      721 ttttggcttg catcacactt ttccgcgagt cagccacact gttttatcga ttattatttt
      781 tctgatttca gaaaccggaa acttacaact gagatgcttt cattttgatt tttttaaatt
      841 tagttttgat ttcagcgatt tgtttgaata taattatgca tgagttggct tttgcatttt
      901 tagataaata agttcacatg ctcggacaaa ttgtgtggtt taatctgtga aagttttgaa
      961 tcttctgttt gaaaaaaaaa acatttttaa tttgtatata cgagtgaatt ggttaggccc
     1021 tggttacgtt ttccagcgaa ttcattcaaa tttcttattt tttctatttt ttcaattaaa
     1081 taatttagtt ctctatcaca tgtgtatgat caaaatttca ttcctaatcg taagcaaatt
     1141 taggctcatc cgtagtataa ttttgttatg aagggcttca cagtaaaagc tcaaactcca
     1201 acatttagca actagcaacc aacaaaaaac caaagaaatg tcagttttga aaagtcgtta
     1261 tttctttgtc ttctcacaat tcacacatca tcttctcgtt ttcctccgtt tttttgtcgt
     1321 gcttccttct tttctttaca catttttcta accttttttt aagtctatcc tctcctaatc
     1381 ctttgtactc tctaatcagt tttgctggtt gtgctgcaca atttttatca gatttatgcg
     1441 cgaaacaaaa gcctctcgcg gagagctccg ccaaatccgg tcttcttttt tccttgccta
     1501 tcggatttca caaacattca gtttgtcact tgacgatgtg ttggaggcat tactactagg
     1561 aagtgttatt catatttttt attcgtttct ttttggtgac taaacgaaat attccgaacg
     1621 ttttcctgat ttcaggcggc ggaaacgaag aagaccacga ataatcaaca ggctgccatt
     1681 gcatccgcca gaagtcgagt gagttctttg aaaatcttta ttagagttat ttgtgacaat
     1741 ttgccacttt ttttttaatg tttaatgaat ctgtaacgaa attaatacca agggtgtcca
     1801 acagaactat ctgagcggtt atttaatttt tagatagtat atttcttccg atcgtaacat
     1861 acatattttc aagcatagta ctgtgattta agttacagtt ttccaatcag tcttgatttt
     1921 aataaaaact tttcaactgc aaaaatgcct ctgaagtatt tttctatatt tttatagttg
     1981 aataattttt tgattggcac aaagaccgcc gagatatgag ccgttaaagt aaagctgcat
     2041 ggattcaata tggtttgtat ttgattggag tcacttacat ggaaatattt gtatttaaat
     2101 ctcatatttt aaaacaagac aaacaccaaa ttaaattttt attaatctga acatttttca
     2161 atcgatttgg taaacaaatt attagatgtt tccctcaaaa attgtaacaa ttcctaaatt
     2221 gtggcactta taataacatc gtcctgaatt ctcaaaaaaa accgaatgct aagagagatt
     2281 tcgtttaact gggacctgta actgaatttt caaatattgg cagtttttga taaactggga
     2341 gggaaatcaa aaaccaaaac tacagtaccc ctcgtgttat aataaattgt cattcactcc
     2401 ttttttgttc ccaacatttg ctcccatctc ctgacgagaa agaacaattt tatgagcacg
     2461 ggaacccgtg ggcgtccatc cgttcctcct gtgcaatttc agcccccttc ctctacattc
     2521 tctgtgttcc cgtttttatc gttttatggg tatcgggaac ggacagatcg cttccatttg
     2581 cttctattcc gtcctcacaa gacgtcccac taactgattt tcttctgatt tcttttcact
     2641 gaaaatggga aagaaagaaa aagacgatga atcgacgact tcttctcagg agaaggtgaa
     2701 gaagaaggat aaggagaaag ttcatctaag gctgaaagat aaagtaaaaa aacttagaaa
     2761 atctaagtcg attgcagtat cttctccagc tgattatatg acttgtcagt gtgaaccatg
     2821 caaaaaagta gctctcgagg taataatttt taaactcaat acgtagttaa gtttgaactc
     2881 cttttaattt acttatcatc taatttaccg tcacaatagt acttccatta gcaattattt
     2941 taaaattaca tgcatagctt caatgttcgt ttttttgcgt tctcatatcg tatgttgtct
     3001 gcctctcgaa tactgactgt ccgactagaa tgtcccggta ggattactgt acggtcactg
     3061 ggaataaact gtttctgaaa tcaatctgaa attccgtaga tcttcttggt tcaatttctt
     3121 attcctcaat ctttggacaa cgtcaccttc agagtcgtcg ttgagtatcc catcattaac
     3181 tagtcaatct ttctctcttt ttcatttccc ccaacttttc tgcatatttt ttgacgacga
     3241 cgacctcgac ggcaacgaga atacatttat ctatttttta gtatagcata tatacatttt
     3301 ctacttttct ctaacccatt tcagacgtat agattttggt tttcgaatgc aactttgaga
     3361 tttttcaaag atcttcagat gggagcagtg gactgtctat ttgatattct taaaatgtac
     3421 tctataaaag atgaaactgt aatgaccaat tccctacata aatattttta actaattggt
     3481 cggatatttg gggatcagag aagtacttgt ttttgcgata ttaataatga tatatttttc
     3541 tagtaaaaaa atgttaagcg atttgtgaat aattaagttg gcttgaaaaa gattttttga
     3601 tagctatgta ttttgttaat ttaaatcaga ttttaaaact ttattattga ctatttatat
     3661 atattatata ttatatattt tcatggttta atcaaaatct attaaattat ttctaagatc
     3721 tctaaatatc gaattctcta aaaagcttca tcaacaaaag catttcaatt atctaacaca
     3781 atgagaataa ttcaagaact gaaatagaaa gaaatctaga aaaaaaagag agtgaggtgc
     3841 cctattaaat cggtgctctc ctctctaaca gaagcgccta cacgcaccca agagagctct
     3901 ttttctttct tttttcggag cacacataca cgcatacact cctcagaagt gtatagagaa
     3961 aatgggagaa gaagcgaaag aagacgaaga agcggttgtg atgtatgcat cgggcgtttc
     4021 tatccctctc cgtgagcaac gagctagtca acccatcgat ccatttttgt gagcacacac
     4081 agagagagac tcaaacacac tcacagcttc acactgagct ccaagagctc tgtagagaga
     4141 agccagagta tgctttcctc gattttccta acgtcttctt cttctgcttt tttctttttc
     4201 ccaacgagtt gtagtccact aacttcaatt tttcctccag agaaagagtt ttagagagac
     4261 agctgataaa tacactagag aaataaaaag aatgataaaa atttgctatt ttaatcagtt
     4321 ccctctcgtt tctctaagtt ttgttgttgg gttgggaaat taaaattgga aaatggaaat
     4381 aacgtttttt tctgtctaat cttgaattct ccagacattt cttatttcct tttttgttgc
     4441 aagtttttct tccttctcta atttttaatc aaagatatct cgctatgaac tttgaatttt
     4501 tgatattttg attctataaa tacataaagt aatctaaatg ctcttttaaa ataaatctac
     4561 agtagcccta ttttcagcct aggatgtctg attccgctgt tgttgccgcc gctgtcgagc
     4621 caaaggtccc aaaggctaag gccgccaagg ccgccaagcc agccgccaag gccgccaagc
     4681 caaccaaagt tgccaaggcc aaggctccag tcgctcaccc accatacatc aacatgatca
     4741 aggaggccat caagcagctc aaggaccgca aaggagcttc caagcaggca atcctcaagt
     4801 tcatctccca aaactacaag ctcggagaca atgtcatcca gatcaatgct catctccgtc
     4861 aggctctcaa gcgtggtgtc accagcaagg cccttgttca agccgccgga tccggagcca
     4921 acggacgttt ccgtgtgcca gagaaggccg ccgccgccaa gaagccagca gcagccaaga
     4981 agccagcagc agccaagaaa ccagcagccg ccaagaaagc cactggagag aagaaggcaa
     5041 agaagccagc cgctgccaag ccaaagaagg ccgccaccgg agataagaag gtcaagaagg
     5101 ccaagagccc aaagaaggtt gccaagccag ctgccaagaa ggtcgccaag tctccagcca
     5161 agaaggccgc tccaaagaag atcgccaagc cagctgccaa gaaggccgcc aagccagccg
     5221 ccaaggccgc atgcatggtg agcaagggcg aggagaataa catggccatc atcaaggagt
     5281 tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     5341 gcgagggcga gggccgcccc tacgagggct ttcagaccgc taagctgaag gtgaccaagg
     5401 gtggccccct gcccttcgcc tgggacatcc tgtcccctca tttcacctac ggctccaagg
     5461 cctacgtgaa gcaccccgcc gacatccccg actacttcaa gctgtccttc cccgagggct
     5521 tcaagtggga gcgcgtgatg aactacgagg acggcggcgt ggtgaccgtg acccaggact
     5581 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     5641 ccgacggccc cgtgatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     5701 accccgagga cggtgccctg aagggcaaga tcaagatgag gctgaagctg aaggacggcg
     5761 gccactacac ctccgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     5821 gcgcctacat cgtcgacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     5881 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     5941 agtaaaagct ttggaattca tctcgcgccc gtgcctctga cttctaagtc caattactct
     6001 tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa
     6061 aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat
     6121 tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct
     6181 ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat
     6241 gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc
     6301 ttatcatatg ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc
     6361 tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc
     6421 aatcaagtga aagtttatga aattaatttt cctgcttttg ctttttgggg tttcccctat
     6481 tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa atcacaagta ttgatgagca
     6541 cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct cagtggaagg tgagtagaag
     6601 ttgataattt gaaagtggag tagtgtctat ggggtttttg ccttaaatga cagaatacat
     6661 tcccaatata ccaaacataa ctgtttgggc ccggtaccca gcttttgttc cctttagtga
     6721 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6781 ccgctcacaa ttccacacaa catacgagcc gggagcataa agtgtaaagc ctggggtgcc
     6841 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6901 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6961 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7021 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7081 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7141 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7201 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7261 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7321 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7381 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7441 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7501 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7561 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7621 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7681 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7741 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7801 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7861 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7921 ttaatcagtg aggcacctat ctcagcgatc tgcctatttc gttcatccat agttgcctga
     7981 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8041 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8101 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8161 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8221 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8281 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8341 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8401 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8461 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8521 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8581 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8641 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8701 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8761 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8821 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8881 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     8941 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     9001 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     9061 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     9121 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     9181 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     9241 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     9301 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     9361 attcgccatt caggctgcgc aactgttggg aagggc
//
