LOCUS       sequence-241967-        9546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Localized Pan-Neuronal mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9546)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS1-rab-3P::his-24::mCherry
FEATURES             Location/Qualifiers
     source          1..9546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..88
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     80..97
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     81..97
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     107..126
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        107..125
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6839..6859)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6839..6857)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6878..6894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6878..6894)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6891..6913)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6902..6918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6926..6932,6933..6950,6951..6956))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6971..6992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7109..7126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7280..7868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7360..7379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8039..8830,8831..8899))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8662..8681
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8900..9004)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9030..9485)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9167..9188)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9379..9398
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
       61 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
      121 atagggcgaa ttggagctcc accgcggact agtggatccc ccgggctgca ggcgagtttt
      181 gactggcttt caaaaactga gtgattacta attatttttg aaaaaattaa cagttgtgat
      241 atgatattta gacatttttg caatatataa tttgtttgaa aaaaaaacat tttttaatgg
      301 tcttcgaagc tataatagtt tttcgtaaaa ttttaaataa aacagctgac agatatccac
      361 attcaataat tgaaagtatg cagttctctt tttttttgtc aaatagaaaa aaagtaacag
      421 atggtcagat ataatctagg ttaatctgac tctggtcatc gatgaactca ttttcatcat
      481 aatcattctt ctttcaaatg gtctgttcgg aattcgaata ttcaataaat ggatatctta
      541 ttagatgctc cggatttatt ccaccaaact tccaagacgt aagttcaatt taaacaaaat
      601 tactatagct tgaaaaaatg agaaaaaacg agcagataaa aaaataacag aaaaaatcca
      661 taaaaatgac gagggttccg tgcgaaaatc gtcattgttt ttggcttgca tcacactttt
      721 ccgcgagtca gccacactgt tttatcgatt attatttttc tgatttcaga aaccggaaac
      781 ttacaactga gatgctttca ttttgatttt tttaaattta gttttgattt cagcgatttg
      841 tttgaatata attatgcatg agttggcttt tgcattttta gataaataag ttcacatgct
      901 cggacaaatt gtgtggttta atctgtgaaa gttttgaatc ttctgtttga aaaaaaaaac
      961 atttttaatt tgtatatacg agtgaattgg ttaggccctg gttacgtttt ccagcgaatt
     1021 cattcaaatt tcttattttt tctatttttt caattaaata atttagttct ctatcacatg
     1081 tgtatgatca aaatttcatt cctaatcgta agcaaattta ggctcatccg tagtataatt
     1141 ttgttatgaa gggcttcaca gtaaaagctc aaactccaac atttagcaac tagcaaccaa
     1201 caaaaaacca aagaaatgtc agttttgaaa agtcgttatt tctttgtctt ctcacaattc
     1261 acacatcatc ttctcgtttt cctccgtttt tttgtcgtgc ttccttcttt tctttacaca
     1321 tttttctaac ctttttttaa gtctatcctc tcctaatcct ttgtactctc taatcagttt
     1381 tgctggttgt gctgcacaat ttttatcaga tttatgcgcg aaacaaaagc ctctcgcgga
     1441 gagctccgcc aaatccggtc ttcttttttc cttgcctatc ggatttcaca aacattcagt
     1501 ttgtcacttg acgatgtgtt ggaggcatta ctactaggaa gtgttattca tattttttat
     1561 tcgtttcttt ttggtgacta aacgaaatat tccgaacgtt ttcctgattt caggcggcgg
     1621 aaacgaagaa gaccacgaat aatcaacagg ctgccattgc atccgccaga agtcgagtga
     1681 gttctttgaa aatctttatt agagttattt gtgacaattt gccacttttt ttttaatgtt
     1741 taatgaatct gtaacgaaat taataccaag ggtgtccaac agaactatct gagcggttat
     1801 ttaattttta gatagtatat ttcttccgat cgtaacatac atattttcaa gcatagtact
     1861 gtgatttaag ttacagtttt ccaatcagtc ttgattttaa taaaaacttt tcaactgcaa
     1921 aaatgcctct gaagtatttt tctatatttt tatagttgaa taattttttg attggcacaa
     1981 agaccgccga gatatgagcc gttaaagtaa agctgcatgg attcaatatg gtttgtattt
     2041 gattggagtc acttacatgg aaatatttgt atttaaatct catattttaa aacaagacaa
     2101 acaccaaatt aaatttttat taatctgaac atttttcaat cgatttggta aacaaattat
     2161 tagatgtttc cctcaaaaat tgtaacaatt cctaaattgt ggcacttata ataacatcgt
     2221 cctgaattct caaaaaaaac cgaatgctaa gagagatttc gtttaactgg gacctgtaac
     2281 tgaattttca aatattggca gtttttgata aactgggagg gaaatcaaaa accaaaacta
     2341 cagtacccct cgtgttataa taaattgtca ttcactcctt ttttgttccc aacatttgct
     2401 cccatctcct gacgagaaag aacaatttta tgagcacggg aacccgtggg cgtccatccg
     2461 ttcctcctgt gcaatttcag cccccttcct ctacattctc tgtgttcccg tttttatcgt
     2521 tttatgggta tcgggaacgg acagatcgct tccatttgct tctattccgt cctcacaaga
     2581 cgtcccacta actgattttc ttctgatttc ttttcactga aaatgggaaa gaaagaaaaa
     2641 gacgatgaat cgacgacttc ttctcaggag aaggtgaaga agaaggataa ggagaaagtt
     2701 catctaaggc tgaaagataa agtaaaaaaa cttagaaaat ctaagtcgat tgcagtatct
     2761 tctccagctg attatatgac ttgtcagtgt gaaccatgca aaaaagtagc tctcgaggta
     2821 ataattttta aactcaatac gtagttaagt ttgaactcct tttaatttac ttatcatcta
     2881 atttaccgtc acaatagtac ttccattagc aattatttta aaattacatg catagcttca
     2941 atgttcgttt ttttgcgttc tcatatcgta tgttgtctgc ctctcgaata ctgactgtcc
     3001 gactagaatg tcccggtagg attactgtac ggtcactggg aataaactgt ttctgaaatc
     3061 aatctgaaat tccgtagatc ttcttggttc aatttcttat tcctcaatct ttggacaacg
     3121 tcaccttcag agtcgtcgtt gagtatccca tcattaacta gtcaatcttt ctctcttttt
     3181 catttccccc aacttttctg catatttttt gacgacgacg acctcgacgg caacgagaat
     3241 acatttatct attttttagt atagcatata tacattttct acttttctct aacccatttc
     3301 agacgtatag attttggttt tcgaatgcaa ctttgagatt tttcaaagat cttcagatgg
     3361 gagcagtgga ctgtctattt gatattctta aaatgtactc tataaaagat gaaactgtaa
     3421 tgaccaattc cctacataaa tatttttaac taattggtcg gatatttggg gatcagagaa
     3481 gtacttgttt ttgcgatatt aataatgata tatttttcta gtaaaaaaat gttaagcgat
     3541 ttgtgaataa ttaagttggc ttgaaaaaga ttttttgata gctatgtatt ttgttaattt
     3601 aaatcagatt ttaaaacttt attattgact atttatatat attatatatt atatattttc
     3661 atggtttaat caaaatctat taaattattt ctaagatctc taaatatcga attctctaaa
     3721 aagcttcatc aacaaaagca tttcaattat ctaacacaat gagaataatt caagaactga
     3781 aatagaaaga aatctagaaa aaaaagagag tgaggtgccc tattaaatcg gtgctctcct
     3841 ctctaacaga agcgcctaca cgcacccaag agagctcttt ttctttcttt tttcggagca
     3901 cacatacacg catacactcc tcagaagtgt atagagaaaa tgggagaaga agcgaaagaa
     3961 gacgaagaag cggttgtgat gtatgcatcg ggcgtttcta tccctctccg tgagcaacga
     4021 gctagtcaac ccatcgatcc atttttgtga gcacacacag agagagactc aaacacactc
     4081 acagcttcac actgagctcc aagagctctg tagagagaag ccagagtatg ctttcctcga
     4141 ttttcctaac gtcttcttct tctgcttttt tctttttccc aacgagttgt agtccactaa
     4201 cttcaatttt tcctccagag aaagagtttt agagagacag ctgataaata cactagagaa
     4261 ataaaaagaa tgataaaaat ttgctatttt aatcagttcc ctctcgtttc tctaagtttt
     4321 gttgttgggt tgggaaatta aaattggaaa atggaaataa cgtttttttc tgtctaatct
     4381 tgaattctcc agacatttct tatttccttt tttgttgcaa gtttttcttc cttctctaat
     4441 ttttaatcaa agatatctcg ctatgaactt tgaatttttg atattttgat tctataaata
     4501 cataaagtaa tctaaatgct cttttaaaat aaatctacag tagccctatt ttcagcctag
     4561 gatgtctgat tccgctgttg ttgccgccgc tgtcgagcca aaggtcccaa aggctaaggc
     4621 cgccaaggcc gccaagccag ccgccaaggc cgccaagcca accaaagttg ccaaggccaa
     4681 ggctccagtc gctcacccac catacatcaa catgatcaag gaggccatca agcagctcaa
     4741 ggaccgcaaa ggagcttcca agcaggcaat cctcaagttc atctcccaaa actacaagct
     4801 cggagacaat gtcatccaga tcaatgctca tctccgtcag gctctcaagc gtggtgtcac
     4861 cagcaaggcc cttgttcaag ccgccggatc cggagccaac ggacgtttcc gtgtgccaga
     4921 gaaggccgcc gccgccaaga agccagcagc agccaagaag ccagcagcag ccaagaaacc
     4981 agcagccgcc aagaaagcca ctggagagaa gaaggcaaag aagccagccg ctgccaagcc
     5041 aaagaaggcc gccaccggag ataagaaggt caagaaggcc aagagcccaa agaaggttgc
     5101 caagccagct gccaagaagg tcgccaagtc tccagccaag aaggccgctc caaagaagat
     5161 cgccaagcca gctgccaaga aggccgccaa gccagccgcc aaggccgcat gcatggtctc
     5221 aaagggtgaa gaagataaca tggcaattat taaagagttt atgcgtttca aggtgcatat
     5281 ggagggatct gtcaatgggc atgagtttga aattgaaggt gaaggagaag gccgaccata
     5341 tgagggaaca caaaccgcaa aactaaaggt aagtttaaac atatatatac taactaaccc
     5401 tgattattta aattttcagg taactaaagg cggaccatta ccattcgcct gggacatcct
     5461 ctctccacag ttcatgtatg gaagtaaagc ttatgttaaa catccggcag atataccaga
     5521 ttatttgaaa ctttcattcc cggagggttt taagtgggaa cgcgtaatga attttgaaga
     5581 cggaggagtt gttacagtga cgcaagactc aaggtaagtt taaacagttc ggtactaact
     5641 aaccatacat atttaaattt tcagcctcca agatggagaa tttatttata aagtcaaact
     5701 tcgaggaacg aatttcccct cggatggacc tgttatgcag aagaagacta tgggatggga
     5761 agcttcaagt gaaagaatgt accctgaaga cggtgctctt aagggagaga ttaaacaacg
     5821 tcttaaattg aaagatggag gacattacga tgctgaggta agtttaaaca tgattttact
     5881 aactaactaa tctgatttaa attttcaggt gaagacaact tacaaagcca aaaaaccagt
     5941 tcagctgcca ggagcgtaca atgttaatat taaactggat atcacctccc acaacgagga
     6001 ttacactatc gttgagcaat atgaaagagc tgaagggcgg cactcgacag gtggcatgga
     6061 tgaattgtat aagaagcttt ggaattcatc tcgcgcccgt gcctctgact tctaagtcca
     6121 attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt
     6181 attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac
     6241 aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg
     6301 tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca
     6361 cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac
     6421 aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg
     6481 tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg
     6541 aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt
     6601 tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt
     6661 gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg
     6721 agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca
     6781 gaatacattc ccaatatacc aaacataact gtttgggccc ggtacccagc ttttgttccc
     6841 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     6901 attgttatcc gctcacaatt ccacacaaca tacgagccgg gagcataaag tgtaaagcct
     6961 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7021 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7081 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7681 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     7741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7921 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7981 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8041 accaatgctt aatcagtgag gcacctatct cagcgatctg cctatttcgt tcatccatag
     8101 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8161 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8221 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8281 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8341 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8401 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8461 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8521 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8581 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8641 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8701 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8761 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8821 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8881 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8941 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9001 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     9061 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     9121 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     9181 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     9241 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     9301 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     9361 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     9421 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     9481 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     9541 ttcgct
//
