LOCUS       sequence-241971-        9420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Localized Pan-Neuronal mNeptune.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9420)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS1-rab3P::his-24::mNeptune
FEATURES             Location/Qualifiers
     source          1..9420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     44..66
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     58..75
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     59..75
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     85..104
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        85..103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5191..5925
                     /codon_start=1
                     /product="brighter version of the far-red fluorescent 
                     protein mNeptune2 with slightly blue-shifted spectra"
                     /label=mNeptune2.5
                     /translation="MVSKGEELIKENMHTKLYMEGTVNNHHFKCTHEGEGKPYEGTQTN
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     primer_bind     complement(6691..6711)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6691..6709)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6730..6746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6730..6746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6743..6765)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6754..6770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6778..6784,6785..6802,6803..6808))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6823..6844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6961..6978)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7132..7720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7212..7231)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7891..8682,8683..8751))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8514..8533
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8752..8856)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8882..9337)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9019..9040)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9231..9250
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
       61 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctccac
      121 cgcggactag tggatccccc gggctgcagg cgagttttga ctggctttca aaaactgagt
      181 gattactaat tatttttgaa aaaattaaca gttgtgatat gatatttaga catttttgca
      241 atatataatt tgtttgaaaa aaaaacattt tttaatggtc ttcgaagcta taatagtttt
      301 tcgtaaaatt ttaaataaaa cagctgacag atatccacat tcaataattg aaagtatgca
      361 gttctctttt tttttgtcaa atagaaaaaa agtaacagat ggtcagatat aatctaggtt
      421 aatctgactc tggtcatcga tgaactcatt ttcatcataa tcattcttct ttcaaatggt
      481 ctgttcggaa ttcgaatatt caataaatgg atatcttatt agatgctccg gatttattcc
      541 accaaacttc caagacgtaa gttcaattta aacaaaatta ctatagcttg aaaaaatgag
      601 aaaaaacgag cagataaaaa aataacagaa aaaatccata aaaatgacga gggttccgtg
      661 cgaaaatcgt cattgttttt ggcttgcatc acacttttcc gcgagtcagc cacactgttt
      721 tatcgattat tatttttctg atttcagaaa ccggaaactt acaactgaga tgctttcatt
      781 ttgatttttt taaatttagt tttgatttca gcgatttgtt tgaatataat tatgcatgag
      841 ttggcttttg catttttaga taaataagtt cacatgctcg gacaaattgt gtggtttaat
      901 ctgtgaaagt tttgaatctt ctgtttgaaa aaaaaaacat ttttaatttg tatatacgag
      961 tgaattggtt aggccctggt tacgttttcc agcgaattca ttcaaatttc ttattttttc
     1021 tattttttca attaaataat ttagttctct atcacatgtg tatgatcaaa atttcattcc
     1081 taatcgtaag caaatttagg ctcatccgta gtataatttt gttatgaagg gcttcacagt
     1141 aaaagctcaa actccaacat ttagcaacta gcaaccaaca aaaaaccaaa gaaatgtcag
     1201 ttttgaaaag tcgttatttc tttgtcttct cacaattcac acatcatctt ctcgttttcc
     1261 tccgtttttt tgtcgtgctt ccttcttttc tttacacatt tttctaacct ttttttaagt
     1321 ctatcctctc ctaatccttt gtactctcta atcagttttg ctggttgtgc tgcacaattt
     1381 ttatcagatt tatgcgcgaa acaaaagcct ctcgcggaga gctccgccaa atccggtctt
     1441 cttttttcct tgcctatcgg atttcacaaa cattcagttt gtcacttgac gatgtgttgg
     1501 aggcattact actaggaagt gttattcata ttttttattc gtttcttttt ggtgactaaa
     1561 cgaaatattc cgaacgtttt cctgatttca ggcggcggaa acgaagaaga ccacgaataa
     1621 tcaacaggct gccattgcat ccgccagaag tcgagtgagt tctttgaaaa tctttattag
     1681 agttatttgt gacaatttgc cacttttttt ttaatgttta atgaatctgt aacgaaatta
     1741 ataccaaggg tgtccaacag aactatctga gcggttattt aatttttaga tagtatattt
     1801 cttccgatcg taacatacat attttcaagc atagtactgt gatttaagtt acagttttcc
     1861 aatcagtctt gattttaata aaaacttttc aactgcaaaa atgcctctga agtatttttc
     1921 tatattttta tagttgaata attttttgat tggcacaaag accgccgaga tatgagccgt
     1981 taaagtaaag ctgcatggat tcaatatggt ttgtatttga ttggagtcac ttacatggaa
     2041 atatttgtat ttaaatctca tattttaaaa caagacaaac accaaattaa atttttatta
     2101 atctgaacat ttttcaatcg atttggtaaa caaattatta gatgtttccc tcaaaaattg
     2161 taacaattcc taaattgtgg cacttataat aacatcgtcc tgaattctca aaaaaaaccg
     2221 aatgctaaga gagatttcgt ttaactggga cctgtaactg aattttcaaa tattggcagt
     2281 ttttgataaa ctgggaggga aatcaaaaac caaaactaca gtacccctcg tgttataata
     2341 aattgtcatt cactcctttt ttgttcccaa catttgctcc catctcctga cgagaaagaa
     2401 caattttatg agcacgggaa cccgtgggcg tccatccgtt cctcctgtgc aatttcagcc
     2461 cccttcctct acattctctg tgttcccgtt tttatcgttt tatgggtatc gggaacggac
     2521 agatcgcttc catttgcttc tattccgtcc tcacaagacg tcccactaac tgattttctt
     2581 ctgatttctt ttcactgaaa atgggaaaga aagaaaaaga cgatgaatcg acgacttctt
     2641 ctcaggagaa ggtgaagaag aaggataagg agaaagttca tctaaggctg aaagataaag
     2701 taaaaaaact tagaaaatct aagtcgattg cagtatcttc tccagctgat tatatgactt
     2761 gtcagtgtga accatgcaaa aaagtagctc tcgaggtaat aatttttaaa ctcaatacgt
     2821 agttaagttt gaactccttt taatttactt atcatctaat ttaccgtcac aatagtactt
     2881 ccattagcaa ttattttaaa attacatgca tagcttcaat gttcgttttt ttgcgttctc
     2941 atatcgtatg ttgtctgcct ctcgaatact gactgtccga ctagaatgtc ccggtaggat
     3001 tactgtacgg tcactgggaa taaactgttt ctgaaatcaa tctgaaattc cgtagatctt
     3061 cttggttcaa tttcttattc ctcaatcttt ggacaacgtc accttcagag tcgtcgttga
     3121 gtatcccatc attaactagt caatctttct ctctttttca tttcccccaa cttttctgca
     3181 tattttttga cgacgacgac ctcgacggca acgagaatac atttatctat tttttagtat
     3241 agcatatata cattttctac ttttctctaa cccatttcag acgtatagat tttggttttc
     3301 gaatgcaact ttgagatttt tcaaagatct tcagatggga gcagtggact gtctatttga
     3361 tattcttaaa atgtactcta taaaagatga aactgtaatg accaattccc tacataaata
     3421 tttttaacta attggtcgga tatttgggga tcagagaagt acttgttttt gcgatattaa
     3481 taatgatata tttttctagt aaaaaaatgt taagcgattt gtgaataatt aagttggctt
     3541 gaaaaagatt ttttgatagc tatgtatttt gttaatttaa atcagatttt aaaactttat
     3601 tattgactat ttatatatat tatatattat atattttcat ggtttaatca aaatctatta
     3661 aattatttct aagatctcta aatatcgaat tctctaaaaa gcttcatcaa caaaagcatt
     3721 tcaattatct aacacaatga gaataattca agaactgaaa tagaaagaaa tctagaaaaa
     3781 aaagagagtg aggtgcccta ttaaatcggt gctctcctct ctaacagaag cgcctacacg
     3841 cacccaagag agctcttttt ctttcttttt tcggagcaca catacacgca tacactcctc
     3901 agaagtgtat agagaaaatg ggagaagaag cgaaagaaga cgaagaagcg gttgtgatgt
     3961 atgcatcggg cgtttctatc cctctccgtg agcaacgagc tagtcaaccc atcgatccat
     4021 ttttgtgagc acacacagag agagactcaa acacactcac agcttcacac tgagctccaa
     4081 gagctctgta gagagaagcc agagtatgct ttcctcgatt ttcctaacgt cttcttcttc
     4141 tgcttttttc tttttcccaa cgagttgtag tccactaact tcaatttttc ctccagagaa
     4201 agagttttag agagacagct gataaataca ctagagaaat aaaaagaatg ataaaaattt
     4261 gctattttaa tcagttccct ctcgtttctc taagttttgt tgttgggttg ggaaattaaa
     4321 attggaaaat ggaaataacg tttttttctg tctaatcttg aattctccag acatttctta
     4381 tttccttttt tgttgcaagt ttttcttcct tctctaattt ttaatcaaag atatctcgct
     4441 atgaactttg aatttttgat attttgattc tataaataca taaagtaatc taaatgctct
     4501 tttaaaataa atctacagta gccctatttt cagcctagga tgtctgattc cgctgttgtt
     4561 gccgccgctg tcgagccaaa ggtcccaaag gctaaggccg ccaaggccgc caagccagcc
     4621 gccaaggccg ccaagccaac caaagttgcc aaggccaagg ctccagtcgc tcacccacca
     4681 tacatcaaca tgatcaagga ggccatcaag cagctcaagg accgcaaagg agcttccaag
     4741 caggcaatcc tcaagttcat ctcccaaaac tacaagctcg gagacaatgt catccagatc
     4801 aatgctcatc tccgtcaggc tctcaagcgt ggtgtcacca gcaaggccct tgttcaagcc
     4861 gccggatccg gagccaacgg acgtttccgt gtgccagaga aggccgccgc cgccaagaag
     4921 ccagcagcag ccaagaagcc agcagcagcc aagaaaccag cagccgccaa gaaagccact
     4981 ggagagaaga aggcaaagaa gccagccgct gccaagccaa agaaggccgc caccggagat
     5041 aagaaggtca agaaggccaa gagcccaaag aaggttgcca agccagctgc caagaaggtc
     5101 gccaagtctc cagccaagaa ggccgctcca aagaagatcg ccaagccagc tgccaagaag
     5161 gccgccaagc cagccgccaa ggccgcatgc atggtgagca agggcgagga gctgatcaag
     5221 gagaacatgc acacgaagct gtacatggaa ggcaccgtga acaaccacca cttcaagtgc
     5281 acccacgaag gggagggcaa gccctacgag ggcacccaga ccaacaggat taaggtggtg
     5341 gagggaggcc ccctgccgtt cgcattcgac atcctggcca cctgctttat gtacgggagc
     5401 aagaccttca tcaaccacac ccagggcatc cccgatttct ttaagcagtc cttccctgag
     5461 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgcttac cgttacccag
     5521 gacaccagcc tccaggacgg ctgcttgatc tacaacgtca agctcagagg ggtgaacttc
     5581 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccag taccgagacc
     5641 ctgtaccccg ctgacggcgg cctggaaggc agatgcgaca tggccctgaa gctcgtgggc
     5701 gggggccacc tgcactgcaa cctgaagacc acatacagat ccaagaaacc cgctaagaac
     5761 ctcaagatgc ccggcgtcta ctttgtggac cgcagactgg aaagaatcaa ggaggccgac
     5821 aatgagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc
     5881 aaactggggc acaaacttaa tggcatggac gagctgtaca agtaaaagct ttggaattca
     5941 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct
     6001 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
     6061 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga
     6121 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
     6181 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
     6241 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     6301 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg
     6361 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga
     6421 aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg
     6481 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa
     6541 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag
     6601 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa
     6661 ctgtttgggc ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     6721 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6781 catacgagcc gggagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6841 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6901 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6961 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7021 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7081 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7141 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7201 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7261 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7321 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7381 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7441 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7501 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7561 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7621 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7681 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7741 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7801 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7861 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7921 ctcagcgatc tgcctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7981 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8041 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8101 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8161 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8221 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8281 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8341 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8401 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8461 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8521 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8581 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8641 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8701 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8761 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8821 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8881 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     8941 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     9001 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     9061 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     9121 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     9181 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     9241 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     9301 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
     9361 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
//
