LOCUS       sequence-241978-        9390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Localized Pan-Neuronal CyOFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9390)
  TITLE     Leifer Lab worm expression plasmids
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAS1-rab-3P::his-24::CyOFP
FEATURES             Location/Qualifiers
     source          1..9390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5732..6433
                     /codon_start=1
                     /product="orange-red fluorescent protein that is excitable 
                     by cyan light and suitable as an acceptor for blue-emitting
                     luciferase (Chu et al., 2016)"
                     /label=CyOFP1
                     /translation="MVSKGEELIKENMRSKLYLEGSVNGHQFKCTHEGEGKPYEGKQTN
                     RIKVVEGGPLPFAFDILATHFMYGSKVFIKYPADLPDYFKQSFPEGFTWERVMVFEDGG
                     VLTATQDTSLQDGELIYNVKVRGVNFPANGPVMQKKTLGWEPSTETMYPADGGLEGRCD
                     KALKLVGGGHLHVNFKTTYKSKKPVKMPGVHYVDRRLERIKEADNETYVEQYEHAVARY
                     SNLGGGMDELYK"
     primer_bind     complement(7202..7222)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7202..7220)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7241..7257)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7241..7257)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7254..7276)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7265..7281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7289..7295,7296..7313,7314..7319))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7334..7355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7472..7489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7643..8231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7723..7742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8402..9193,9194..9262))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9025..9044
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9263..9367)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggacta gtggatcccc cgggctgcag gcgagttttg actggctttc aaaaactgag
      721 tgattactaa ttatttttga aaaaattaac agttgtgata tgatatttag acatttttgc
      781 aatatataat ttgtttgaaa aaaaaacatt ttttaatggt cttcgaagct ataatagttt
      841 ttcgtaaaat tttaaataaa acagctgaca gatatccaca ttcaataatt gaaagtatgc
      901 agttctcttt ttttttgtca aatagaaaaa aagtaacaga tggtcagata taatctaggt
      961 taatctgact ctggtcatcg atgaactcat tttcatcata atcattcttc tttcaaatgg
     1021 tctgttcgga attcgaatat tcaataaatg gatatcttat tagatgctcc ggatttattc
     1081 caccaaactt ccaagacgta agttcaattt aaacaaaatt actatagctt gaaaaaatga
     1141 gaaaaaacga gcagataaaa aaataacaga aaaaatccat aaaaatgacg agggttccgt
     1201 gcgaaaatcg tcattgtttt tggcttgcat cacacttttc cgcgagtcag ccacactgtt
     1261 ttatcgatta ttatttttct gatttcagaa accggaaact tacaactgag atgctttcat
     1321 tttgattttt ttaaatttag ttttgatttc agcgatttgt ttgaatataa ttatgcatga
     1381 gttggctttt gcatttttag ataaataagt tcacatgctc ggacaaattg tgtggtttaa
     1441 tctgtgaaag ttttgaatct tctgtttgaa aaaaaaaaca tttttaattt gtatatacga
     1501 gtgaattggt taggccctgg ttacgttttc cagcgaattc attcaaattt cttatttttt
     1561 ctattttttc aattaaataa tttagttctc tatcacatgt gtatgatcaa aatttcattc
     1621 ctaatcgtaa gcaaatttag gctcatccgt agtataattt tgttatgaag ggcttcacag
     1681 taaaagctca aactccaaca tttagcaact agcaaccaac aaaaaaccaa agaaatgtca
     1741 gttttgaaaa gtcgttattt ctttgtcttc tcacaattca cacatcatct tctcgttttc
     1801 ctccgttttt ttgtcgtgct tccttctttt ctttacacat ttttctaacc tttttttaag
     1861 tctatcctct cctaatcctt tgtactctct aatcagtttt gctggttgtg ctgcacaatt
     1921 tttatcagat ttatgcgcga aacaaaagcc tctcgcggag agctccgcca aatccggtct
     1981 tcttttttcc ttgcctatcg gatttcacaa acattcagtt tgtcacttga cgatgtgttg
     2041 gaggcattac tactaggaag tgttattcat attttttatt cgtttctttt tggtgactaa
     2101 acgaaatatt ccgaacgttt tcctgatttc aggcggcgga aacgaagaag accacgaata
     2161 atcaacaggc tgccattgca tccgccagaa gtcgagtgag ttctttgaaa atctttatta
     2221 gagttatttg tgacaatttg ccactttttt tttaatgttt aatgaatctg taacgaaatt
     2281 aataccaagg gtgtccaaca gaactatctg agcggttatt taatttttag atagtatatt
     2341 tcttccgatc gtaacataca tattttcaag catagtactg tgatttaagt tacagttttc
     2401 caatcagtct tgattttaat aaaaactttt caactgcaaa aatgcctctg aagtattttt
     2461 ctatattttt atagttgaat aattttttga ttggcacaaa gaccgccgag atatgagccg
     2521 ttaaagtaaa gctgcatgga ttcaatatgg tttgtatttg attggagtca cttacatgga
     2581 aatatttgta tttaaatctc atattttaaa acaagacaaa caccaaatta aatttttatt
     2641 aatctgaaca tttttcaatc gatttggtaa acaaattatt agatgtttcc ctcaaaaatt
     2701 gtaacaattc ctaaattgtg gcacttataa taacatcgtc ctgaattctc aaaaaaaacc
     2761 gaatgctaag agagatttcg tttaactggg acctgtaact gaattttcaa atattggcag
     2821 tttttgataa actgggaggg aaatcaaaaa ccaaaactac agtacccctc gtgttataat
     2881 aaattgtcat tcactccttt tttgttccca acatttgctc ccatctcctg acgagaaaga
     2941 acaattttat gagcacggga acccgtgggc gtccatccgt tcctcctgtg caatttcagc
     3001 ccccttcctc tacattctct gtgttcccgt ttttatcgtt ttatgggtat cgggaacgga
     3061 cagatcgctt ccatttgctt ctattccgtc ctcacaagac gtcccactaa ctgattttct
     3121 tctgatttct tttcactgaa aatgggaaag aaagaaaaag acgatgaatc gacgacttct
     3181 tctcaggaga aggtgaagaa gaaggataag gagaaagttc atctaaggct gaaagataaa
     3241 gtaaaaaaac ttagaaaatc taagtcgatt gcagtatctt ctccagctga ttatatgact
     3301 tgtcagtgtg aaccatgcaa aaaagtagct ctcgaggtaa taatttttaa actcaatacg
     3361 tagttaagtt tgaactcctt ttaatttact tatcatctaa tttaccgtca caatagtact
     3421 tccattagca attattttaa aattacatgc atagcttcaa tgttcgtttt tttgcgttct
     3481 catatcgtat gttgtctgcc tctcgaatac tgactgtccg actagaatgt cccggtagga
     3541 ttactgtacg gtcactggga ataaactgtt tctgaaatca atctgaaatt ccgtagatct
     3601 tcttggttca atttcttatt cctcaatctt tggacaacgt caccttcaga gtcgtcgttg
     3661 agtatcccat cattaactag tcaatctttc tctctttttc atttccccca acttttctgc
     3721 atattttttg acgacgacga cctcgacggc aacgagaata catttatcta ttttttagta
     3781 tagcatatat acattttcta cttttctcta acccatttca gacgtataga ttttggtttt
     3841 cgaatgcaac tttgagattt ttcaaagatc ttcagatggg agcagtggac tgtctatttg
     3901 atattcttaa aatgtactct ataaaagatg aaactgtaat gaccaattcc ctacataaat
     3961 atttttaact aattggtcgg atatttgggg atcagagaag tacttgtttt tgcgatatta
     4021 ataatgatat atttttctag taaaaaaatg ttaagcgatt tgtgaataat taagttggct
     4081 tgaaaaagat tttttgatag ctatgtattt tgttaattta aatcagattt taaaacttta
     4141 ttattgacta tttatatata ttatatatta tatattttca tggtttaatc aaaatctatt
     4201 aaattatttc taagatctct aaatatcgaa ttctctaaaa agcttcatca acaaaagcat
     4261 ttcaattatc taacacaatg agaataattc aagaactgaa atagaaagaa atctagaaaa
     4321 aaaagagagt gaggtgccct attaaatcgg tgctctcctc tctaacagaa gcgcctacac
     4381 gcacccaaga gagctctttt tctttctttt ttcggagcac acatacacgc atacactcct
     4441 cagaagtgta tagagaaaat gggagaagaa gcgaaagaag acgaagaagc ggttgtgatg
     4501 tatgcatcgg gcgtttctat ccctctccgt gagcaacgag ctagtcaacc catcgatcca
     4561 tttttgtgag cacacacaga gagagactca aacacactca cagcttcaca ctgagctcca
     4621 agagctctgt agagagaagc cagagtatgc tttcctcgat tttcctaacg tcttcttctt
     4681 ctgctttttt ctttttccca acgagttgta gtccactaac ttcaattttt cctccagaga
     4741 aagagtttta gagagacagc tgataaatac actagagaaa taaaaagaat gataaaaatt
     4801 tgctatttta atcagttccc tctcgtttct ctaagttttg ttgttgggtt gggaaattaa
     4861 aattggaaaa tggaaataac gtttttttct gtctaatctt gaattctcca gacatttctt
     4921 atttcctttt ttgttgcaag tttttcttcc ttctctaatt tttaatcaaa gatatctcgc
     4981 tatgaacttt gaatttttga tattttgatt ctataaatac ataaagtaat ctaaatgctc
     5041 ttttaaaata aatctacagt agccctattt tcagcctagg atgtctgatt ccgctgttgt
     5101 tgccgccgct gtcgagccaa aggtcccaaa ggctaaggcc gccaaggccg ccaagccagc
     5161 cgccaaggcc gccaagccaa ccaaagttgc caaggccaag gctccagtcg ctcacccacc
     5221 atacatcaac atgatcaagg aggccatcaa gcagctcaag gaccgcaaag gagcttccaa
     5281 gcaggcaatc ctcaagttca tctcccaaaa ctacaagctc ggagacaatg tcatccagat
     5341 caatgctcat ctccgtcagg ctctcaagcg tggtgtcacc agcaaggccc ttgttcaagc
     5401 cgccggatcc ggagccaacg gacgtttccg tgtgccagag aaggccgccg ccgccaagaa
     5461 gccagcagca gccaagaagc cagcagcagc caagaaacca gcagccgcca agaaagccac
     5521 tggagagaag aaggcaaaga agccagccgc tgccaagcca aagaaggccg ccaccggaga
     5581 taagaaggtc aagaaggcca agagcccaaa gaaggttgcc aagccagctg ccaagaaggt
     5641 cgccaagtct ccagccaaga aggccgctcc aaagaagatc gccaagccag ctgccaagaa
     5701 ggccgccaag ccagccgcca aggccgcatg catggtgagc aagggcgagg agctgatcaa
     5761 ggagaacatg agaagcaagc tgtacctgga aggcagcgtg aacggccacc agttcaagtg
     5821 cacccacgaa ggggagggca agccctacga gggcaagcag accaacagga tcaaggtggt
     5881 ggagggaggc cccctgccgt tcgcattcga catcctggcc acccacttta tgtacgggag
     5941 caaggtgttc atcaagtacc ccgccgacct ccccgattat tttaagcagt ccttccctga
     6001 gggcttcaca tgggagagag tcatggtgtt cgaagacggg ggcgtgctga ccgccaccca
     6061 ggacaccagc ctccaggacg gcgagctcat ctacaacgtc aaggtcagag gggtgaactt
     6121 cccagccaac ggccccgtga tgcagaagaa aacactgggc tgggagccca gcaccgagac
     6181 catgtacccc gctgacggcg gcctggaagg cagatgcgac aaggccctga agctcgtggg
     6241 cgggggccac ctgcacgtca acttcaagac cacatacaag tccaagaaac ccgtgaagat
     6301 gcccggcgtc cactacgtgg accgcagact ggaaagaatc aaggaggccg acaacgagac
     6361 ctacgtcgag cagtacgagc acgctgtggc cagatactcc aacctgggcg gaggcatgga
     6421 cgagctgtac aagtaaaagc tttggaattc atctcgcgcc cgtgcctctg acttctaagt
     6481 ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt
     6541 gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta agtcacctct
     6601 aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag tcgaaaaaaa
     6661 ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa tgttctgtgt
     6721 acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga aaaaaccgca
     6781 cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc
     6841 acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt
     6901 tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg
     6961 gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa aatcacaagt
     7021 attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc tcagtggaag
     7081 gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt gccttaaatg
     7141 acagaataca ttcccaatat accaaacata actgtttggg cccggtaccc agcttttgtt
     7201 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7261 gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag
     7321 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7381 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7441 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7501 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7561 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7621 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7681 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7741 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7801 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7861 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7921 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7981 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8041 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8101 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8161 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8221 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8281 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8341 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8401 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgcctattt cgttcatcca
     8461 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8521 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8581 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8641 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8701 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8761 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8821 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8881 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8941 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9001 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9061 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9121 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9181 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9241 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9301 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9361 ttccgcgcac atttccccga aaagtgccac
//
