LOCUS       sequence-241986-        3759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for sense in situ probe in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Castro JP, Yancoskie MN, Marchini M, Belohlavy S, Hiramatsu L, Kucka
            M, Beluch WH, Naumann R, Skuplik I, Cobb J, Barton NH, Rolian C, 
            Chan YF
  TITLE     An integrative genomic analysis of the Longshanks selection 
            experiment for longer limbs in mice.
  JOURNAL   Elife. 2019 Jun 6;8:e42014. doi: 10.7554/eLife.42014.
  PUBMED    31169497
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-06-6"; volume: "8:e42014. doi"; pages: " 
            10.7554/eLife.42014"
COMMENT     Sequence Label: pJET1.2_Rab28_Sense
FEATURES             Location/Qualifiers
     source          1..3759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    96..117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(132..137,138..155,156..162)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(1429..1448)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1430..1448)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1754..1772)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1840..1944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1945..2013,2014..2805)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2163..2182)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2976..3564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3465..3484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3718..3735
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
       61 gactggaaag caattggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      121 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggaat tatgagcgga
      181 taataatttc acacaggagg tttaaacttt aaacatgtca aaagagacgt cttttgttaa
      241 gaatgctgag gaacttgcaa agcaaaaaat ggatgctatt aaccctgaac tttcttcaaa
      301 atttaaattt ttaataaaat tcctgtctca gtttcctgaa gcttgctcta aacctcgttc
      361 aaaaaaaatg cagaataaag ttggtcaaga ggaacatatt gaatatttag ctcgtagttt
      421 tcatgagagt cgattgccaa gaaaacccac gccacctaca acggttcctg atgaggtggt
      481 tagcatagtt cttaatataa gttttaatat acagcctgaa aatcttgaga gaataaaaga
      541 agaacatcga ttttccatgg cagctgagaa tattgtagga gatcttctag aaagatgatc
      601 atcaaagctt ggagcagctt catatctggt taaatttttt atatctggga aacaaagaag
      661 tgaagagaga aggaagcaac atggttcttc tggagtgagg aggcagtctc aatccagaga
      721 atggactctc cattcggatt tcaaaacgtg tggttccaaa tcgcactcct tccggtccag
      781 ggagctgcct gatccctcag gcaactaaag gatgaaggag cagagcagga ggaagtgtga
      841 gctggctacg gagacagtca ctgataaaac acgttcctag gcagctgggg cccaattcag
      901 gggaagagca gccagaacta tcaatacagt gtgtgctcac tgcactgcac acatggagct
      961 ccgaggaggg ttaacagttc ttgacaaggg ctcctggttg tagtttacaa tatctgcctt
     1021 caccactctc tgtgactgtt ctatttctgc tttgtttaat ttgattccca ggatttctgc
     1081 tgcaactttc tgaaaacaca ggaagacaga gtctcctgtc ttggctgaaa caaagtggct
     1141 actaaaacca ttttcctggc aaaatcgtaa gtgcttatca gcttttactg ttcgcatatg
     1201 ctccaagtca attttattgc ccactaaagc aaccaggggc tgagtttctg actcttcact
     1261 tactgtcttc accacagaat accaatcttc caaattctca aagctttggt agtttgtaat
     1321 atcatatacc aagaggattc cctgtgctcc atagatgtat ttatccaaca tcttgccacc
     1381 tatcttgctg aaaaactcga gccatccgga agatctggcg gccgctctcc ctatagtgag
     1441 tcgtattacg ccggatggat atggtgttca ggcacaagtg ttaaagcagt tgattttatt
     1501 cactatgatg aaaaaaacaa tgaatggaac ctgctccaag ttaaaaatag agataatacc
     1561 gaaaactcat cgagtagtaa gattagagat aatacaacaa taaaaaaatg gtttagaact
     1621 tactcacagc gtgatgctac taattgggac aattttccag atgaagtatc atctaagaat
     1681 ttaaatgaag aagacttcag agcttttgtt aaaaattatt tggcaaaaat aatataattc
     1741 ggctgcaggg gcggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1801 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1861 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1921 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     1981 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2041 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2101 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2161 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2221 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2281 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2341 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2401 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2461 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2521 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2581 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2641 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2701 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2761 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2821 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2881 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2941 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3001 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3061 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3121 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3181 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3241 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3301 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3361 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3421 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3481 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3541 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3601 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3661 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3721 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgc
//
