LOCUS       sequence-241993-        8538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human PHB attached to a V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Ortiz MV, Ahmed S, Burns M, Henssen AG, Hollmann TJ, MacArthur I, 
            Gunasekera S, Gaewsky L, Bradwin G, Ryan J, Letai A, He Y, Naranjo 
            A, Chi YY, LaQuaglia M, Heaton T, Cifani P, Dome JS, Gadd S, Perlman
            E, Mullen E, Steen H, Kentsis A
  TITLE     Prohibitin is a prognostic marker and therapeutic target to block 
            chemotherapy resistance in Wilms' tumor.
  JOURNAL   JCI Insight. 2019 Aug 8;4(15). pii: 127098. doi: 
            10.1172/jci.insight.127098. eCollection 2019 Aug 8.
  PUBMED    31391345
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2019-08-8"; volume: "4(15). pii: 127098. doi"; 
            pages: " 10.1172/jci.insight.127098. eCollection 2019 Aug 8"
COMMENT     Sequence Label: plx304-PHB-V5
FEATURES             Location/Qualifiers
     source          1..8538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..30
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    147..168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(183..188,189..206,207..213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    221..237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     226..248
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     245..261
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     245..261
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     280..300
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        282..300
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        328..554
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             555..735
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    782..907
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1400..1633
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1818..1862
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2037..2537
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2456..2474
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2549..2944
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3004..3121
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3238..3541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3542..3745
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3696..3716
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3768..3792
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4609..4633)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4635..4676
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4718..5306
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4771..4791)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5189..5200)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5378..5611
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5689..5810
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5726..5745)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5780..5799
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5850..5985
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6005..6024)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6006..6024)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6034..6051)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6034..6050)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6043..6065)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6192..6647
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6279..6298)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6489..6510
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6673..6777
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6778..6846,6847..7638)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6996..7015)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7809..8397
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8298..8317
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
       61 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      121 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      181 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      241 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
      301 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
      361 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
      421 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
      481 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
      541 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
      601 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      661 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      721 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
      781 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
      841 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
      901 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
      961 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     1021 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     1081 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     1141 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     1201 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     1261 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     1321 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     1381 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     1441 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     1501 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     1561 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     1621 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     1681 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     1741 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     1801 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     1861 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     1921 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     1981 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccttgc
     2041 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     2101 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     2161 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     2221 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     2281 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     2341 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     2401 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     2461 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     2521 ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag aagaatccac
     2581 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     2641 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     2701 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     2761 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     2821 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     2881 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     2941 gggcctgcag ctgcagtagt aagaattcta gatcttgaga caaatggcag tattcatcca
     3001 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     3061 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     3121 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggg
     3181 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     3241 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     3301 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     3361 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     3421 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     3481 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     3541 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     3601 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     3661 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     3721 gtgggaggtc tatataagca gagctctctg gctaactgtc gggatcaaca agtttgtaca
     3781 aaaaagttgg catggctgcc aaagtgtttg agtccattgg caagtttggc ctggccttag
     3841 ctgttgcagg aggcgtggtg aactctgcct tatataatgt ggatgctggg cacagagctg
     3901 tcatctttga ccgattccgt ggagtgcagg acattgtggt aggggaaggg actcattttc
     3961 tcatcccgtg ggtacagaaa ccaattatct ttgactgccg ttctcgacca cgtaatgtgc
     4021 cagtcatcac tggtagcaaa gatttacaga atgtcaacat cacactgcgc atcctcttcc
     4081 ggcctgtcgc cagccagctt cctcgcatct tcaccagcat cggagaggac tatgatgagc
     4141 gtgtgctgcc gtccatcaca actgagatcc tcaagtcagt ggtggctcgc tttgatgctg
     4201 gagaactaat cacccagaga gagctggtct ccaggcaggt gagcgacgac cttacagagc
     4261 gagccgccac ctttgggctc atcctggatg acgtgtcctt gacacatctg accttcggga
     4321 aggagttcac agaagcggtg gaagccaaac aggtggctca gcaggaagca gagagggcca
     4381 gatttgtggt ggaaaaggct gagcaacaga aaaaggcggc catcatctct gctgagggcg
     4441 actccaaggc agctgagctg attgccaact cactggccac tgcaggggat ggcctgatcg
     4501 agctgcgcaa gctggaagct gcagaggaca tcgcgtacca gctctcacgc tctcggaaca
     4561 tcacctacct gccagcgggg cagtccgtgc tcctccagct gccccagtgc ccaactttct
     4621 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
     4681 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg attacaaaat
     4741 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4801 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4861 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4921 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4981 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5041 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5101 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     5161 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5221 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5281 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     5341 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     5401 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     5461 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5521 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5581 cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt
     5641 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     5701 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5761 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5821 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5881 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5941 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     6001 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     6061 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6121 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     6181 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6241 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6301 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6361 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6421 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6481 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6541 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6601 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     6661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6721 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6781 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6841 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6901 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6961 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7021 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7081 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7141 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7201 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7261 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7321 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7381 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7441 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7501 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7561 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7621 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7681 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7741 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7801 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7861 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     7921 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7981 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8041 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8101 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8161 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8221 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8281 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8341 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8401 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8461 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8521 accgctcgcc gcagccga
//
