LOCUS       sequence-242011-       10197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Yu RY, Wang X, Pixley FJ, Yu JJ, Dent AL, Broxmeyer HE, Stanley ER, 
            Ye BH
  TITLE     BCL-6 negatively regulates macrophage proliferation by suppressing 
            autocrine IL-6 production.
  JOURNAL   Blood. 2005 Feb 15. 105(4):1777-84.
  PUBMED    15507530
REFERENCE   2  (bases 1 to 10197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2005
            Feb 15. 105(4):1777-84."
COMMENT     Sequence Label: MSCV-BCL6-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..10197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1502..1525
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3821..4370
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3965..3982)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4192..4211
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4373..4375,4376..4378,4379..5092)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4373..4375,4376..4378,4379..5089)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4418..4439)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4679..4698)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5026..5047
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5254..5768
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5930..5946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5954..5960,5961..5978,5979..5984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5999..6020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6137..6154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6308..6896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6388..6407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8405..9196,9197..9265))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9028..9047
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9266..9370)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9438..9456
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9494..9516)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9616..9635
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9829..9851
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9979..9998
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctcccagagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tggccgcgtc gaagcttgca tgcctgcagg
     1441 tcgacaccag gtttgagcaa aattttggac tgtgaagcaa ggcattggtg aagacaaaat
     1501 ggactacaag gatgatgacg ataaggcaat ggcctcgccg gctgacagct gtatccagtt
     1561 cacccgccat gccagtgatg ttcttctcaa ccttaatcgt ctccggagtc gagacatctt
     1621 gactgatgtt gtcattgttg tgagccgtga gcagtttaga gcccataaaa cggtcctcat
     1681 ggcctgcagt ggcctgttct atagcatctt tacagaccag ttgaaatgca accttagtgt
     1741 gatcaatcta gatcctgaga tcaaccctga gggattctgc atcctcctgg acttcatgta
     1801 cacatctcgg ctcaatttgc gggagggcaa catcatggct gtgatggcca cggctatgta
     1861 cctgcagatg gagcatgttg tggacacttg ccggaagttt attaaggcca gtgaagcaga
     1921 gatggtttct gccatcaagc ctcctcgtga agagttcctc aacagccgga tgctgatgcc
     1981 ccaagacatc atggcctatc ggggtcgtga ggtggtggag aacaacctgc cactgaggag
     2041 cgcccctggg tgtgagagca gagcctttgc ccccagcctg tacagtggcc tgtccacacc
     2101 gccagcctct tattccatgt acagccacct ccctgtcagc agcctcctct tctccgatga
     2161 ggagtttcgg gatgtccgga tgcctgtggc caaccccttc cccaaggagc gggcactccc
     2221 atgtgatagt gccaggccag tccctggtga gtacagccgg ccgactttgg aggtgtcccc
     2281 caatgtgtgc cacagcaata tctattcacc caaggaaaca atcccagaag aggcacgaag
     2341 tgatatgcac tacagtgtgg ctgagggcct caaacctgct gccccctcag cccgaaatgc
     2401 cccctacttc ccttgtgaca aggccagcaa agaagaagag agaccctcct cggaagatga
     2461 gattgccctg catttcgagc cccccaatgc acccctgaac cggaagggtc tggttagtcc
     2521 acagagcccc cagaaatctg actgccagcc caactcgccc acagagtcct gcagcagtaa
     2581 gaatgcctgc atcctccagg cttctggctc ccctccagcc aagagcccca ctgaccccaa
     2641 agcctgcaac tggaagaaat acaagttcat cgtgctcaac agcctcaacc agaatgccaa
     2701 accagagggg cctgagcagg ctgagctggg ccgcctttcc ccacgagcct acacggcccc
     2761 acctgcctgc cagccaccca tggagcctga gaaccttgac ctccagtccc caaccaagct
     2821 gagtgccagc ggggaggact ccaccatccc acaagccagc cggctcaata acatcgttaa
     2881 caggtccatg acgggctctc cccgcagcag cagcgagagc cactcaccac tctacatgca
     2941 ccccccgaag tgcacgtcct gcggctctca gtccccacag catgcagaga tgtgcctcca
     3001 caccgctggc cccacgttcc ctgaggagat gggagagacc cagtctgagt actcagattc
     3061 tagctgtgag aacggggcct tcttctgcaa tgagtgtgac tgccgcttct ctgaggaggc
     3121 ctcactcaag aggcacacgc tgcagaccca cagtgacaaa ccctacaagt gtgaccgctg
     3181 ccaggcctcc ttccgctaca agggcaacct cgccagccac aagaccgtcc ataccggtga
     3241 gaaaccctat cgttgcaaca tctgtggggc ccagttcaac cggccagcca acctgaaaac
     3301 ccacactcga attcactctg gagagaagcc ctacaaatgc gaaacctgcg gagccagatt
     3361 tgtacaggtg gcccacctcc gtgcccatgt gcttatccac actggtgaga agccctatcc
     3421 ctgtgaaatc tgtggcaccc gtttccggca ccttcagact ctgaagagcc acctgcgaat
     3481 ccacacagga gagaaacctt accattgtga gaagtgtaac ctgcatttcc gtcacaaaag
     3541 ccagctgcga cttcacttgc gccagaagca tggcgccatc accaacacca aggtgcaata
     3601 ccgcgtgtca gccactgacc tgcctccgga gctccccaaa gcctgctgaa gcatggagtg
     3661 ttgatgcttt cgtctccagc cccttctcag aatctaccca aaggatactg taacacttta
     3721 caatgttcat cccatgatgt agtgcctctt tcatccacta ggggtactcg acgcggccaa
     3781 ttcgttaacc tcgagcggga tcaattccgc ccccccccta acgttactgg ccgaagccgc
     3841 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     3901 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     3961 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     4021 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     4081 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     4141 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     4201 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     4261 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     4321 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata ccatggtgag
     4381 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     4441 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     4501 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     4561 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     4621 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     4681 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     4741 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     4801 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     4861 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     4921 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4981 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5041 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     5101 cgactctaga gtcgacctgc aggcatgcaa gcttcaggta gccggctaac gttaacaacc
     5161 ggtacctcta gaactatagc tagcatgcgc aaatttaaag cgctgatatc gataaaataa
     5221 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     5281 aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg agaatagaga
     5341 agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca ggatatctgt
     5401 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg
     5461 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     5521 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     5581 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
     5641 agactgcgtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     5701 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     5761 tctttcatgg gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa
     5821 tattaagtaa tcctgactca attagccact gttttgaatc cacatactcc aatactcctg
     5881 aaatagttca ttatggacag cgcagaaaga gctggggaga attgtgaaat tgttatccgc
     5941 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6001 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6061 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6121 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6181 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6241 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6301 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6361 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6421 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6481 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6541 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6601 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6661 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6721 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6781 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6841 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6901 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6961 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7021 gttttaaatc aatctaaagt actgatgaat cccctaatga tttttatcaa aatcattaag
     7081 ttaaggtaga tacacatctt gtcatatgat caaatggttt cgccaaaaat caataatcag
     7141 acaacaaaat gtgcgaactc gatattttac acgactctct ttaccaattc tgccccgaat
     7201 tacacttaaa acgactcaac agcttaacgt tggcttgcca cgccttactt gactgtaaaa
     7261 ctctcactct taccgaactt ggccgtaacc tgccaaccaa agcgagaaca aaacataaca
     7321 tcaaacgaat cgaccgattg ttaggtaatc gtcacctcca caaagagcga ctcgctgtat
     7381 accgttggca tgctagcttt atctgttcgg gcaatacgat gcccattgta cttgttgact
     7441 ggtctgatat ccgtgagcaa aaacggctta tggtattgcg agcttcagtc gcactacacg
     7501 gtcgttctgt tactctttat gagaaagcgt tcccgctttc agagcaatgt tcaaagaaag
     7561 ctcatgacca atttctagcc gaccttgcga gcattctacc gagtaacacc acaccgctca
     7621 ttgtcagtga tgctggcttt aaagtgccat ggtataaatc cgttgagaag ctgggttggt
     7681 actggttaag tcgagtaaga ggaaaagtac aatatgcaga cctaggagcg gaaaactgga
     7741 aacctatcag caacttacat gatatgtcat ctagtcactc aaagacttta ggctataaga
     7801 ggctgactaa aagcaatcca atctcatgcc aaattctatt gtataaatct cgctctaaag
     7861 gccgaaaaaa tcagcgctcg acacggactc attgtcacca cccgtcacct aaaatctact
     7921 cagcgtcggc aaaggagcca tggattctag caactaactt acctgttgaa attcgaacac
     7981 ccaaacaact tgttaatatc tattcgaagc gaatgcagat tgaagaaacc ttccgagact
     8041 tgaaaagtcc tgcctacgga ctaggcctac gccatagccg aacgagcagc tcagagcgtt
     8101 ttgatatcat gctgctaatc gccctgatgc ttcaactaac atgttggctt gcgggcgttc
     8161 atgctcagaa acaaggttgg gacaagcact tccaggctaa cacagtcaga aatcgaaacg
     8221 tactctcaac agttcgctta ggcatggaag ttttgcggca ttctggctac acaataacaa
     8281 gggaagactt actcgtggct gcaaccctac tagctcaaaa tttattcaca catggttacg
     8341 ctttggggaa attatgaggg gatctctcag tctaaagtat atatgagtaa acttggtctg
     8401 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8461 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8521 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8581 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8641 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8701 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8761 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8821 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8881 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8941 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9001 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9061 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9121 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9181 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9241 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9301 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9361 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     9421 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     9481 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     9541 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     9601 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     9661 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     9721 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     9781 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     9841 gttgtaaaac gacggcgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc
     9901 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     9961 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
    10021 gccggtgatg ccggccacga tgcgtccggc gtagaggcga ttagtccaat ttgttaaaga
    10081 caggatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat
    10141 agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa agggggg
//
