LOCUS       sequence-242017-        7307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soma-localized C1V1(t/t) for dense expression in excitatory neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Chettih SN, Harvey CD
  TITLE     Single-neuron perturbations reveal feature-specific competition in 
            V1.
  JOURNAL   Nature. 2019 Mar 6. pii: 10.1038/s41586-019-0997-6. doi: 
            10.1038/s41586-019-0997-6.
  PUBMED    30842660
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Mar 6. pii: 10.1038/s41586-019-0997-6. doi: 
            10.1038/s41586-019-0997-6."
COMMENT     Sequence Label: pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1
FEATURES             Location/Qualifiers
     source          1..7307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   37..166
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        185..1473
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             2750..3442
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(2880..2898)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3148..3167
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3470..4058
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3523..3543)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3941..3952)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4090..4566
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4289..4308)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4606..4746
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4606..4735
                     /label=AAV2 ITR
     rep_origin      4821..5276
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4908..4927)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5118..5139
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5293..5312)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5412..5434
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5472..5490)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5558..5662
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5663..5731,5732..6523)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5881..5900)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6694..7282
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7183..7202
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggttcctggc cttttgctgg ccttttgctc acatgtcctg caggcagctg cgcgctcgct
       61 cgctcactga ggccgcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag
      121 cgagcgcgca gagagggagt ggccaactcc atcactaggg gttcctgcgg ccgcacgcgt
      181 ttaacattat ggccttaggt cacttcatct ccatggggtt cttcttctga ttttctagaa
      241 aatgagatgg gggtgcagag agcttcctca gtgacctgcc cagggtcaca tcagaaatgt
      301 cagagctaga acttgaactc agattactaa tcttaaattc catgccttgg gggcatgcaa
      361 gtacgatata cagaaggagt gaactcatta gggcagatga ccaatgagtt taggaaagaa
      421 gagtccaggg cagggtacat ctacaccacc cgcccagccc tgggtgagtc cagccacgtt
      481 cacctcatta tagttgcctc tctccagtcc taccttgacg ggaagcacaa gcagaaactg
      541 ggacaggagc cccaggagac caaatcttca tggtccctct gggaggatgg gtggggagag
      601 ctgtggcaga ggcctcagga ggggccctgc tgctcagtgg tgacagatag gggtgagaaa
      661 gcagacagag tcattccgtc agcattctgg gtctgtttgg tacttcttct cacgctaagg
      721 tggcggtgtg atatgcacaa tggctaaaaa gcagggagag ctggaaagaa acaaggacag
      781 agacagaggc caagtcaacc agaccaattc ccagaggaag caaagaaacc attacagaga
      841 ctacaagggg gaagggaagg agagatgaat tagcttcccc tgtaaacctt agaacccagc
      901 tgttgccagg gcaacggggc aatacctgtc tcttcagagg agatgaagtt gccagggtaa
      961 ctacatcctg tctttctcaa ggaccatccc agaatgtggc acccactagc cgttaccata
     1021 gcaactgcct ctttgcccca cttaatccca tcccgtctgt taaaagggcc ctatagttgg
     1081 aggtggggga ggtaggaaga gcgatgatca cttgtggact aagtttgttc gcatcccctt
     1141 ctccaacccc ctcagtacat caccctgggg gaacagggtc cacttgctcc tgggcccaca
     1201 cagtcctgca gtattgtgta tataaggcca gggcaaagag gagcaggttt taaagtgaaa
     1261 ggcaggcagg tgttggggag gcagttaccg gggcaacggg aacagggcgt ttcggaggtg
     1321 gttgccatgg ggacctggat gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc
     1381 ctgctcagaa gccccaagct cgtcagtcaa gccggttctc cgtttgcact caggagcacg
     1441 ggcaggcgag tggcccctag ttctgggggc agctctagag cggtaccgga tccccgggta
     1501 ccggtaggcc accatgtcgc ggaggccatg gcttcttgcc ctagcgctgg cagtggcgct
     1561 ggcggccggc agcgcaggag cctcgactgg cagtgacgcg acggtgccgg tcgcgactca
     1621 ggatggcccc gactacgttt tccaccgtgc ccacgagcgc atgctcttcc aaacctcata
     1681 cactcttgag aacaatggtt ctgttatttg catcccgaac aacggccagt gcttctgctt
     1741 ggcttggctt aaatccaacg gaacaaatgc cgagaagttg gctgccaaca ttctgcagtg
     1801 gattactttt gcgctttcag cgctctgcct gatgttctac ggctaccaga cctggaagtc
     1861 tacttgcggc tgggagacca tttacgtggc cacgatcgag atgatcaagt tcatcatcga
     1921 gtatttccat gagtttgacg aacctgcggt gatctactca tccaacggca acaagaccgt
     1981 gtggcttcgt tacgcgacct ggctgctcac ctgcccggtc cttctgatcc atctgagcaa
     2041 cctcacaggc ctgaaggacg attatagcaa aaggactatg ggcctgttgg tttctgatgt
     2101 gggatgcatc gtgtggggcg caaccagcgc catgtgtacg gggtggacga agatcctgtt
     2161 cttcctcatc tcattgagct atggtatgta tacctatttt catgctgcta aagtttatat
     2221 cgaagcattc cacacagttc caaaagggat ttgtcgagaa ctggtccgag tgatggcctg
     2281 gacattcttt gtggcttggg gaatgtttcc agtcctgttt ctgctgggca cggaaggatt
     2341 cggtcatatc agcccttatg gatctgccat tgggcactcc atcctcgacc tgattgcaaa
     2401 gaacatgtgg ggtgtgctgg ggaattacct gcgcgtcaaa atccacgagc acatcctgtt
     2461 gtatggcgac atcagaaaga agcagaaaat tacgatcgcc ggccaagaga tggaggttga
     2521 gacactggtg gctgaagagg aggacgcggc cgcccagtcc cagcccatcc tcaacaccaa
     2581 ggagatggcc ccgcagagca agcctccaga ggagctggag atgagcagca tgcccagccc
     2641 cgtggcccct ctgcccgcac gcacggaggg cgtcatcgac atgcggagca tgtccagcat
     2701 tgacagcttc atcagctgtg ccacggactt ccctgaagcc accagattcg tgagcaaggg
     2761 cgaggcagtg atcaaggagt tcatgcggtt caaggtgcac atggagggct ccatgaacgg
     2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     2881 caagctgaag gtgaccaagg gtggccccct gcccttctcc tgggacatcc tgtcccctca
     2941 gttcatgtac ggctccaggg ccttcaccaa gcaccccgcc gacatccccg actactataa
     3001 gcagtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc
     3061 cgtgaccgtg acccaggaca cctccctgga ggacggcacc ctgatctaca aggtgaagct
     3121 ccgcggcacc aacttccctc ctgacggccc cgtaatgcag aagaagacaa tgggctggga
     3181 agcgtccacc gagcggttgt accccgagga cggcgtgctg aagggcgaca ttaagatggc
     3241 cctgcgcctg aaggacggcg gccgctacct ggcggacttc aagaccacct acaaggccaa
     3301 gaagcccgtg cagatgcccg gcgcctacaa cgtcgaccgc aagttggaca tcacctccca
     3361 caacgaggac tacaccgtgg tggaacagta cgaacgctcc gagggccgcc actccaccgg
     3421 cggcatggac gagctgtaca agtaagaatt cgatatcaag cttatcgata atcaacctct
     3481 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3541 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3601 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     3661 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     3721 tgccaccacc tgtcagctcc tttccggaac tttcgctttc cccctcccta ttgccacggc
     3781 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     3841 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc
     3901 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     3961 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4021 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag cgctgctcga
     4081 gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     4141 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     4201 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     4261 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     4321 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     4381 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     4441 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     4501 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     4561 gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga
     4621 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     4681 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     4741 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4801 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4861 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
     4921 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4981 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     5041 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5101 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
     5161 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
     5221 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     5281 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     5341 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     5401 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     5461 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     5521 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5581 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5641 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5701 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5761 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5821 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5881 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5941 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6001 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6061 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6121 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6181 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6241 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6301 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6361 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6421 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6481 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6541 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6601 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6661 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6721 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6781 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6841 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6901 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6961 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7021 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7081 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7141 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7201 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7261 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttac
//
