LOCUS       sequence-242059-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG-HA-tagged RNF68 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Rona G, Roberti D, Yin Y, Pagan JK, Homer H, Sassani E, Zeke A, 
            Busino L, Rothenberg E, Pagano M
  TITLE     PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin 
            ligase to sites of DNA damage controls H2A.Z loading.
  JOURNAL   Elife. 2018 Jul 9;7. pii: 38771. doi: 10.7554/eLife.38771.
  PUBMED    29985131
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-9"; volume: "7. pii: 38771. doi"; pages: " 
            10.7554/eLife.38771"
COMMENT     Sequence Label: FLAG-HA-RNF68
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggactaca aggacgacga tgacaagctc gatggaggat acccctacga cgtgcccgac
       61 tacgccgaag gaagatctgc gtctcctcag gggggccaga ttgcgatcgc gatgaggctt
      121 cggaaccagc tccagtcagt gtacaagatg gacccgctac ggaacgagga ggaggttcga
      181 gtgaagatca aagacttgaa tgaacacatt gtttgctgcc tatgcgccgg ctacttcgtg
      241 gatgccacca ccatcacaga gtgtcttcat actttctgca agagttgtat tgtgaagtac
      301 ctccaaacta gcaagtactg ccccatgtgc aacattaaga tccacgagac acagccactg
      361 ctcaacctca aactggaccg ggtcatgcag gacatcgtgt ataagctggt gcctggcttg
      421 caagacagtg aagagaaacg gattcgggaa ttctaccagt cccgaggttt ggaccgggtc
      481 acccagccca ctggggaaga gccagcactg agcaacctcg gcctcccctt cagcagcttt
      541 gaccactcta aagcccacta ctatcgctat gatgagcagt tgaacctgtg cctggagcgg
      601 ctgagttctg gcaaagacaa gaataaaagc gtcctgcaga acaagtatgt ccgatgttct
      661 gttagagctg aggtacgcca tctccggagg gtcctgtgtc accgcttgat gctaaaccct
      721 cagcatgtgc agctcctttt tgacaatgaa gttctccctg atcacatgac aatgaagcag
      781 atatggctct cccgctggtt cggcaagcca tcccctttgc ttttacaata cagtgtgaaa
      841 gagaagagga ggtag
//
