LOCUS       sequence-242086-       10905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary plant vector for transient tGFP (with intron) and fLUC (with 
            intron) expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB115 - pL2_pSB90_fLUC-I_tGFP-I
FEATURES             Location/Qualifiers
     source          1..10905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        445..790
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          1262..1450
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             complement(1758..1775)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     terminator      1799..2051
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2501..2846
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(3003..3021)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3081..3269
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      4791..5043
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5109..5133
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5413..6042
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6471..7544
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7610..7804
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        7866..7970
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7971..8765
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10090..10678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10579..10598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10814..10838
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgatgt atcacctctc gcaagaattc aagcttggag gtcaacatgg tggagcacga
       61 cactctggtc tactccaaaa atgtcaaaga tacagtctca gaagatcaaa gggctattga
      121 gacttttcaa caaaggataa tttcgggaaa cctcctcgga ttccattgcc cagctatctg
      181 tcacttcatc gaaaggacag tagaaaagga aggtggctcc tacaaatgcc atcattgcga
      241 taaaggaaag gctatcattc aagatctctc tgccgacagt ggtcccaaag atggaccccc
      301 acccacgagg agcatcgtgg aaaaagaaga ggttccaacc acgtctacaa agcaagtgga
      361 ttgatgtgat aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
      421 agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
      481 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
      541 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
      601 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt
      661 tccaaccacg tctacaaagc aagtggattg atgtgacatc tccactgacg taagggatga
      721 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      781 ggagaggaca cgctcgagta taagagctca tttttacaac aattaccaac aacaacaaac
      841 aacaaacaac attacaatta catttacaat tatcgataca atgagaggat ctggatctga
      901 gtctgatgag tctggacttc ctgctatgga aatcgagtgt agaatcactg gaacccttaa
      961 cggtgttgag ttcgagcttg ttggaggtgg tgagggaact cctgagcagg gaagaatgac
     1021 taacaagatg aagtctacca agggtgctct taccttctct ccataccttc tttctcacgt
     1081 tatgggatac ggattctacc acttcggaac ttacccatct ggatacgaga accctttcct
     1141 tcatgctatc aacaacggtg gatacaccaa cactaggatc gagaagtacg aggatggtgg
     1201 tgttcttcac gttagcttct cttacagata cgaggctgga agagtgatcg gagatttcaa
     1261 ggtaagtttc tgcttctacc tttgatatat atataataat tatcattaat tagtagtaat
     1321 ataatatttc aaatattttt ttcaaaataa aagaatgtag tatatagcaa ttgcttttct
     1381 gtagtttata agtgtgtata ttttaattta taacttttct aatatatgac caaaatttgt
     1441 tgatgtgcag gttatgggaa ctggattccc tgaggattct gttatcttca ccgacaagat
     1501 catcaggtct aacgctactg ttgagcatct tcatcctatg ggagataacg atctcgatgg
     1561 atctttcacc agaaccttct cacttagaga tggtggttac tactcttctg tggtggattc
     1621 tcacatgcac ttcaagtctg ctatccaccc ttctatcctt caaaacggtg gacctatgtt
     1681 cgctttcaga agagttgagg aagatcactc taacaccgag cttggaatcg ttgagtacca
     1741 acatgctttc aagacccctg atgctgatgc tggtgaggaa tgataagctt gtcaagcaga
     1801 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     1861 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     1921 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     1981 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     2041 gttactagat cgacgctact agcaacatac gctggattac gaattcccat ggggaggtca
     2101 acatggtgga gcacgacact ctggtctact ccaaaaatgt caaagataca gtctcagaag
     2161 atcaaagggc tattgagact tttcaacaaa ggataatttc gggaaacctc ctcggattcc
     2221 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca
     2281 aatgccatca ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc gacagtggtc
     2341 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt
     2401 ctacaaagca agtggattga tgtgataaca tggtggagca cgacactctg gtctactcca
     2461 aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt caacaaagga
     2521 taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga
     2581 cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca
     2641 ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     2701 tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt gacatctcca
     2761 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
     2821 gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt tacaacaatt
     2881 accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc gatacaatgg
     2941 aggacgccaa aaacataaag aaaggcccgg cgccattcta tcctctagag gatggaaccg
     3001 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     3061 ttacagatgc acatatcgag gtaagtttct gcttctacct ttgatatata tataataatt
     3121 atcattaatt agtagtaata taatatttca aatatttttt tcaaaataaa agaatgtagt
     3181 atatagcaat tgcttttctg tagtttataa gtgtgtatat tttaatttat aacttttcta
     3241 atatatgacc aaaatttgtt gatgtgcagg tgaacatcac gtacgcggaa tacttcgaaa
     3301 tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg
     3361 tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg
     3421 gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatga
     3481 acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa attttgaacg
     3541 tgcaaaaaaa attaccaata atccagaaaa ttattatcat ggattctaaa acggattacc
     3601 agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat
     3661 acgattttgt accagagtcc tttgatcgtg acaaaacaat tgcactgata atgaattcct
     3721 ctggatctac tgggttacct aagggtgtgg cccttccgca tagaactgcc tgcgtcagat
     3781 tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa
     3841 gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg
     3901 gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttttacga tcccttcagg
     3961 attacaaaat tcaaagtgcg ttgctagtac caaccctatt ttcattcttc gccaaaagca
     4021 ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggg ggcgcacctc
     4081 tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca tcttccaggg atacgacaag
     4141 gatatgggct cactgagact acatcagcta ttctgattac acccgagggg gatgataaac
     4201 cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg
     4261 ggaaaacgct gggcgttaat cagagaggcg aattatgtgt cagaggacct atgattatgt
     4321 ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac
     4381 attctggaga catagcttac tgggacgagg acgaacactt cttcatagtt gaccgcttga
     4441 agtctttaat taaatacaaa ggatatcagg tggcccccgc tgaattggaa tcgatattgt
     4501 tacaacaccc caacatcttc gacgcgggcg tggcaggtct gcccgacgat gacgccggtg
     4561 aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg
     4621 tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg
     4681 tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc
     4741 tcataaaggc caagaagggc ggaaagtcca aattgtaagc ttgtcaagca gatcgttcaa
     4801 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     4861 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     4921 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     4981 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     5041 atcgacgctc agagaggatg cacatgtgac cgagggacac gaagtgatcc gtttaaacta
     5101 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     5161 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgccagc
     5221 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     5281 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     5341 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     5401 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     5461 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     5521 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     5581 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     5641 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     5701 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     5761 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     5821 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     5881 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     5941 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     6001 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     6061 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     6121 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     6181 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     6241 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     6301 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     6361 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     6421 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     6481 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     6541 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     6601 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     6661 cgtcgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     6721 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     6781 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     6841 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     6901 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     6961 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     7021 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     7081 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     7141 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     7201 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     7261 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     7321 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     7381 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     7441 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     7501 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     7561 tagggcaaat tgccctagca ggggaaaaag gtcgaaaaag cttctttcct gtggatagca
     7621 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     7681 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     7741 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     7801 cataactgtc tggccagcgc acagccgaac agctgcaaaa agcgcccaca tcaaggctcc
     7861 gagtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7921 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atggctaaaa
     7981 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     8041 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     8101 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     8161 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     8221 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     8281 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     8341 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     8401 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaggacactc
     8461 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     8521 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     8581 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     8641 gcgtccggtc gctcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     8701 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     8761 tttagctgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8821 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8881 gtgagttttc gttctagctg taacctcgaa gcgtttcact tgtaacaacg attgagaatt
     8941 tttgtcataa aattgaaata cttggttcgc atttttgtca tccgcggtca gccgcaattc
     9001 tgacgaactg cccatttagc tggagatgat tgtacatcct tcacgtgaaa atttctcaag
     9061 cgctgtgaac aagggttcag attttagatt gaaaggtgag ccgttgaaac acgttcttct
     9121 tgtcgatgac gacgtcgcta tgcggcatct tattattgaa taccttacga tccacgcctt
     9181 caaagtgacc gcggtagccg acagcaccca gttcacaaga gtactctctt ccgcgacggt
     9241 cgatgtcgtg gttgttgatc tagatttagg tcgtgaagat gggctcgaga tcgttcgtaa
     9301 tctggcggca aagtctgata ttccaatcat aattatcagt ggcgaccgcc ttgaggagac
     9361 ggataaagtt gttgcactcg agctaggagc aagtgatttt atcgctaagc cgttcagtat
     9421 cagagagttt ctagcacgca ttcgggttgc cttgcgcgtg cgccccaacg ttgtccgctc
     9481 caaagaccga cggtcttttt gttttactga ctggacactt aatctcaggc aacgtcgctt
     9541 gatgtccgaa gctggcggtg aggtgaaact tacggcaggt gagttcaatc ttctcctcgc
     9601 gtttttagag aaaccccgcg acgttctatc gcgcgagcaa cttctcattg ccagtcgagt
     9661 acgcgacgag gaggtttatg acaggagtat agatgttctc attttgaggc tgcgccgcaa
     9721 acttgaggcg gatccgtcaa gccctcaact gataaaaaca gcaagaggtg ccggttattt
     9781 ctttgacgcg gacgtgcagg tttcgcacgg ggggacgatg gcagcctgag ccaattgcat
     9841 ttgcctctta attatctggc tcaaagggtg actgaggagt aagcgatgtg cccatcacac
     9901 tgaccaccaa gacgggggtt cgttttgaag atcacccttc agacccaagg ccagtgtgcg
     9961 ttgatcgcgg ccacactgca aattcacata ctgtcgataa aggggcgttg gacgagaacc
    10021 cattcgtcag gatactggct cgtgatctca ctgagcgtca gaccccgtag aaaagatcaa
    10081 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    10141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    10201 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    10261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    10321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    10381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    10441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    10501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    10681 cgccagcaac gcggcctttt tacggttcct gctcggatct gttggaccgg acagtagtca
    10741 tggttgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
    10801 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
    10861 ataacacatt gcggacgttt ttaatgtact ggggttgaac actct
//
