LOCUS       sequence-242096-       10518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary plant vector for transient TBSV P19 and fLUC (with intron) 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 10518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB116 - pL2_pSB90_fLUC-I_P19
FEATURES             Location/Qualifiers
     source          1..10518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        426..771
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      1393..1645
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2114..2459
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(2616..2634)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2694..2882
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      4404..4656
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4722..4746
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5026..5655
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6084..7157
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7223..7417
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        7479..7583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7584..8378
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9703..10291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10192..10211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10427..10451
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga ggtcaacatg gtggagcacg acactctggt ctactccaaa
       61 aatgtcaaag atacagtctc agaagatcaa agggctattg agacttttca acaaaggata
      121 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
      181 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
      241 caagatctct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
      301 gaaaaagaag aggttccaac cacgtctaca aagcaagtgg attgatgtga taacatggtg
      361 gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga agatcaaagg
      421 gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt ccattgccca
      481 gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta caaatgccat
      541 cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg tcccaaagat
      601 ggacccccac ccacgaggag catcgtggaa aaagaagagg ttccaaccac gtctacaaag
      661 caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct
      721 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctcgagt
      781 ataagagctc atttttacaa caattaccaa caacaacaaa caacaaacaa cattacaatt
      841 acatttacaa ttatcgatac aatggaacga gctatacaag gaaacgatgc tagggaacaa
      901 gcttatggtg aacgttggaa tggaggatca ggaagttcca cttctccctt caaacttcct
      961 gacgaaagtc cgagttggac tgagtggcgg ctacataacg atgagactat ttcgaatcaa
     1021 gataatcccc ttggtttcaa ggaaagctgg ggtttcggga aagttgtatt taagagatat
     1081 ctcagatacg acgggacgga aacttcactg cacagagtcc ttggatcttg gacgggagat
     1141 tcggttaact atgcagcatc tcgatttctc ggtttcgacc agatcggatg tacctatagt
     1201 attcggtttc gaggagttag tgtcaccatt tctggagggt cgcgaactct tcagcatctc
     1261 agtgaaatgg caattcggtc taagcaagaa ctgctacagc ttaccccagt caaagtggaa
     1321 agtgatgtat caagaggatg ccctgaaggt gttgaaacct tcgaagaaga aagcgagtaa
     1381 gcttgtcaag cagatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     1441 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     1501 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     1561 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     1621 gcggtgtcat ctatgttact agatcgacgc tgcaaggttt tgacgggttt actagcaaca
     1681 tacgctggat tacgaattcc catggggagg tcaacatggt ggagcacgac actctggtct
     1741 actccaaaaa tgtcaaagat acagtctcag aagatcaaag ggctattgag acttttcaac
     1801 aaaggataat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     1861 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     1921 ctatcattca agatctctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1981 gcatcgtgga aaaagaagag gttccaacca cgtctacaaa gcaagtggat tgatgtgata
     2041 acatggtgga gcacgacact ctggtctact ccaaaaatgt caaagataca gtctcagaag
     2101 atcaaagggc tattgagact tttcaacaaa ggataatttc gggaaacctc ctcggattcc
     2161 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca
     2221 aatgccatca ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc gacagtggtc
     2281 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt
     2341 ctacaaagca agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc
     2401 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac
     2461 gctcgagtat aagagctcat ttttacaaca attaccaaca acaacaaaca acaaacaaca
     2521 ttacaattac atttacaatt atcgatacaa tggaggacgc caaaaacata aagaaaggcc
     2581 cggcgccatt ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga
     2641 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtaagtt
     2701 tctgcttcta cctttgatat atatataata attatcatta attagtagta atataatatt
     2761 tcaaatattt ttttcaaaat aaaagaatgt agtatatagc aattgctttt ctgtagttta
     2821 taagtgtgta tattttaatt tataactttt ctaatatatg accaaaattt gttgatgtgc
     2881 aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt tcggttggca gaagctatga
     2941 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     3001 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     3061 tttataatga acgtgaattg ctcaacagta tgaacatttc gcagcctacc gtagtgtttg
     3121 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaaattacca ataatccaga
     3181 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     3241 tcacatctca tctacctccc ggttttaatg aatacgattt tgtaccagag tcctttgatc
     3301 gtgacaaaac aattgcactg ataatgaatt cctctggatc tactgggtta cctaagggtg
     3361 tggcccttcc gcatagaact gcctgcgtca gattctcgca tgccagagat cctatttttg
     3421 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     3481 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     3541 ttgaagaaga gctgttttta cgatcccttc aggattacaa aattcaaagt gcgttgctag
     3601 taccaaccct attttcattc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     3661 atttacacga aattgcttct gggggcgcac ctctttcgaa agaagtcggg gaagcggttg
     3721 caaaacgctt ccatcttcca gggatacgac aaggatatgg gctcactgag actacatcag
     3781 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     3841 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcagagag
     3901 gcgaattatg tgtcagagga cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     3961 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     4021 aggacgaaca cttcttcata gttgaccgct tgaagtcttt aattaaatac aaaggatatc
     4081 aggtggcccc cgctgaattg gaatcgatat tgttacaaca ccccaacatc ttcgacgcgg
     4141 gcgtggcagg tctgcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     4201 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     4261 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     4321 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaagt
     4381 ccaaattgta agcttgtcaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat
     4441 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     4501 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     4561 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     4621 aattatcgcg cgcggtgtca tctatgttac tagatcgacg ctcagagagg atgcacatgt
     4681 gaccgaggga cacgaagtga tccgtttaaa ctatcagtgt ttgacaggat atattggcgg
     4741 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     4801 ggtttatccg ttcgtccatt tgtatgtgcc agccgtgcgg ctgcatgaaa tcctggccgg
     4861 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     4921 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     4981 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     5041 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     5101 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     5161 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     5221 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     5281 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     5341 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     5401 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     5461 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     5521 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     5581 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     5641 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     5701 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     5761 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     5821 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     5881 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     5941 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     6001 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
     6061 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     6121 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     6181 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     6241 gcaaagaatc ccggcaaccg ccggcagccg gtgcgtcgtc gattaggaag ccgcccaagg
     6301 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     6361 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     6421 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     6481 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     6541 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     6601 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     6661 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     6721 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     6781 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     6841 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     6901 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     6961 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     7021 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     7081 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     7141 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     7201 aaggtcgaaa aagcttcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     7261 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     7321 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     7381 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     7441 aacagctgca aaaagcgccc acatcaaggc tccgagtgcg cggaacccct atttgtttat
     7501 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7561 aataatattg aaaaaggaag agtatggcta aaatgagaat atcaccggaa ttgaaaaaac
     7621 tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata
     7681 agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga
     7741 ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg
     7801 ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg
     7861 ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg
     7921 agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata
     7981 cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg
     8041 atgtggattg cgaaaactgg gaagaggaca ctccatttaa agatccgcgc gagctgtatg
     8101 attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag
     8161 acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg
     8221 gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgctcagg gaggatatcg
     8281 gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga
     8341 aaataaaata ttatatttta ctggatgaat tgttttagct gtcagaccaa gtttactcat
     8401 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8461 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttctag ctgtaacctc
     8521 gaagcgtttc acttgtaaca acgattgaga atttttgtca taaaattgaa atacttggtt
     8581 cgcatttttg tcatccgcgg tcagccgcaa ttctgacgaa ctgcccattt agctggagat
     8641 gattgtacat ccttcacgtg aaaatttctc aagcgctgtg aacaagggtt cagattttag
     8701 attgaaaggt gagccgttga aacacgttct tcttgtcgat gacgacgtcg ctatgcggca
     8761 tcttattatt gaatacctta cgatccacgc cttcaaagtg accgcggtag ccgacagcac
     8821 ccagttcaca agagtactct cttccgcgac ggtcgatgtc gtggttgttg atctagattt
     8881 aggtcgtgaa gatgggctcg agatcgttcg taatctggcg gcaaagtctg atattccaat
     8941 cataattatc agtggcgacc gccttgagga gacggataaa gttgttgcac tcgagctagg
     9001 agcaagtgat tttatcgcta agccgttcag tatcagagag tttctagcac gcattcgggt
     9061 tgccttgcgc gtgcgcccca acgttgtccg ctccaaagac cgacggtctt tttgttttac
     9121 tgactggaca cttaatctca ggcaacgtcg cttgatgtcc gaagctggcg gtgaggtgaa
     9181 acttacggca ggtgagttca atcttctcct cgcgttttta gagaaacccc gcgacgttct
     9241 atcgcgcgag caacttctca ttgccagtcg agtacgcgac gaggaggttt atgacaggag
     9301 tatagatgtt ctcattttga ggctgcgccg caaacttgag gcggatccgt caagccctca
     9361 actgataaaa acagcaagag gtgccggtta tttctttgac gcggacgtgc aggtttcgca
     9421 cggggggacg atggcagcct gagccaattg catttgcctc ttaattatct ggctcaaagg
     9481 gtgactgagg agtaagcgat gtgcccatca cactgaccac caagacgggg gttcgttttg
     9541 aagatcaccc ttcagaccca aggccagtgt gcgttgatcg cggccacact gcaaattcac
     9601 atactgtcga taaaggggcg ttggacgaga acccattcgt caggatactg gctcgtgatc
     9661 tcactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9721 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9781 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9841 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9901 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9961 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    10021 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    10081 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    10141 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    10201 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    10261 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    10321 cctgctcgga tctgttggac cggacagtag tcatggttga tgggctgcct gtatcgagtg
    10381 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    10441 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    10501 actggggttg aacactct
//
