LOCUS       sequence-24217-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  TITLE     T4 Lysozyme Plasmids - Tray 3
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme Y88F
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(717..739)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    728..744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gctantgaaa aaagcccgcc tcattaggcg ggctcagatc tgctcatgnt ttgacagctt
       61 atcatcgata agcttcggca ataaattctt gatatgtttt catatgtttc ctttaaatat
      121 tttaattagt aattgtctat tcaagtaatt gtgaatatac tatcacaatt ccaagagaaa
      181 gtaaacagct ttatagattt ttatacgcgt cccaagtgcc agttctaaac gttgtaatga
      241 ctcgttttgc gcgattaggt gtttgattat accatctact tttagctaag ttaactgctg
      301 cttcatccca gcgtttttgt tgaagcatac gtaaagagtt agtaaatcct gccacaccgg
      361 tttctcccat ttggaaaacc atattaatca atgcacagcg acgaaccgca tcaagagaat
      421 caaaaaccgg ttttaattta gcatttctca gaattccgcg aacagcagca tcaacatcct
      481 gattaaagag tttttcagcc tcatcttttg taattacacc attgcaatta cgcccaatag
      541 ctttatctaa ttcagattta gcagcattaa gtgatggact ttttgtaagc aaatgaccga
      601 tgccaatagt gtaatagcct tctgtgtctt tatagatttt aagtctaaga ccttcatcta
      661 tacgtaacat ttcaaatata ttcataatac ctcctaagca tcgatggatc ctgtttcctg
      721 tgtgaaattg ttatccgctc acaattccac acattatacg agccgatgat tattgtcaca
      781 ggggatgggg agtaagctga nctgttcngg ngaatgtatc cgctcncatc cccatatnga
      841 gcgagat
//
