LOCUS       sequence-242183-        8319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector (pRRL) containing KRAS G12V specific T cell 
            receptor, clone 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8319)
  AUTHORS   Veatch JR, Jesernig BL, Kargl J, Fitzgibbon M, Lee SM, Baik C, 
            Martins R, Houghton AM, Riddell SR
  TITLE     Endogenous CD4(+) T Cells Recognize Neoantigens in Lung Cancer 
            Patients, Including Recurrent Oncogenic KRAS and ERBB2 (Her2) Driver
            Mutations.
  JOURNAL   Cancer Immunol Res. 2019 Jun;7(6):910-922. doi: 
            10.1158/2326-6066.CIR-18-0402. Epub 2019 May 1.
  PUBMED    31043415
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2326-6066.CIR-18-0402"; journalName: "Cancer Immunol Res"; 
            date: "2019-06"; volume: "7"; issue: "6"; pages: "910-922"
COMMENT     Sequence Label: pJV132
FEATURES             Location/Qualifiers
     source          1..8319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             940..996
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    1834..2422
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1887..1907)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2305..2316)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2509..2742
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2814..2935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2851..2870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2905..2924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2975..3110
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3130..3149)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3131..3149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3159..3176)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3159..3175)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3168..3190)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3317..3772
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3404..3423)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3614..3635
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3798..3902
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3903..3971,3972..4763)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4121..4140)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4934..5522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5423..5442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5676..5693
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5810..5831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5846..5851,5852..5869,5870..5876)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5884..5900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5889..5911
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5908..5924
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5908..5924
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5943..5963
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5945..5963
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5991..6217
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6218..6398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6445..6570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7063..7296
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7481..7525
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7792..7909
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atgctttgtt ctttgctggc tctgctgctg ggcaccttct ttggcgtcag aagccagacc
       61 atccaccagt ggcctgctac actggtgcag cctgttggaa gccctctgag cctggaatgt
      121 accgtggaag gcaccagcaa tcccaacctg tactggtaca gacaggccgc tggaagagga
      181 ctgcagctgc tgttttacag cgtcggcatc ggccagatca gcagcgaggt tccacagaat
      241 ctgagcgcca gcagacccca ggacagacag tttatcctga gcagcaagaa gctgctgctg
      301 agcgacagcg gcttctacct gtgtgcttgg agtgctctgg caggcgccag agatacccag
      361 tattttggcc ctggcaccag actgaccgtg ctggaagatc tgaagaacgt gttcccacct
      421 gaggtggccg tgttcgagcc ttctgaggcc gagatcagcc acacacagaa agccacactc
      481 gtgtgtctgg ccaccggctt ttaccccgat cacgtggaac tgtcttggtg ggtcaacggc
      541 aaagaggtgc acagcggcgt ctgcacagat ccccagcctc tgaaagaaca gcccgctctg
      601 aacgacagcc ggtactgcct gtctagcaga ctgagagtgt ccgccacctt ctggcagaac
      661 cccagaaacc acttcagatg ccaggtgcag ttctacggcc tgagcgagaa cgatgagtgg
      721 acccaggata gagccaagcc tgtgacacag atcgtgtctg ccgaagcctg gggcagagcc
      781 gattgtggct ttaccagcga gagctaccag cagggcgtgc tgtctgccac aatcctgtac
      841 gagatcctgc tgggaaaagc cactctgtac gccgtgctgg tgtctgccct ggtgctgatg
      901 gccatggtca agcggaagga tagcagaggc ggaagcggcg ccacaaactt cagcctgctt
      961 aaacaggccg gcgacgtgga agagaacccc ggacctatgc tgctggaact gatccctctg
     1021 ctggggattc acttcgtgct gagaaccgct agagcccaga gcgtgaccca gcctgatatc
     1081 cacatcaccg tgtctgaggg cgcctctctg gaactgcggt gcaattacag ctacggcgcc
     1141 actccttacc tgttttggta cgtgcagagc ccaggccagg gactgcaact cctgctgaag
     1201 tactttagcg gcgacaccct ggtgcagggc atcaagggat tcgaggccga gttcaagaga
     1261 agccagtcca gcttcaacct gcggaagcct tccgtgcatt ggagtgatgc cgccgagtac
     1321 ttttgcgccg tgggcagatc taatagcggc ggctaccaga aagtgacctt tggcatcggc
     1381 accaagctgc aagtgatccc caacattcag aaccccgatc ctgcagtgta ccagctgcgg
     1441 gacagcaaga gcagcgacaa gagcgtgtgc ctgttcaccg acttcgacag ccagaccaac
     1501 gtgtcccaga gcaaggacag cgacgtgtac atcaccgata agtgcgtgct ggacatgcgg
     1561 agcatggact tcaagagcaa cagcgccgtg gcctggtcca acaagagcga cttcgcctgc
     1621 gccaacgcct tcaacaacag cattatcccc gaggacacat tcttcccaag ccccgagagc
     1681 agctgcgacg tgaagctggt ggaaaagagc ttcgagacag acaccaacct gaacttccag
     1741 aacctcagcg tgatcggctt ccggatcctg ctgctgaagg tggccggctt caacctgctg
     1801 atgaccctgc ggctgtggtc cagctgagtc gacaatcaac ctctggatta caaaatttgt
     1861 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     1921 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     1981 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2041 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2101 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2161 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2221 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     2281 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2341 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2401 tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt
     2461 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     2521 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     2581 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2641 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2701 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     2761 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     2821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2941 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3001 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3061 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     3121 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     3181 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3241 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3301 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3361 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3421 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3481 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3541 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3601 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3661 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3721 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     3781 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3841 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3901 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3961 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4021 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4081 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4141 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4201 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4261 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4321 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4381 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4441 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4501 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4561 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4621 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4681 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4741 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4801 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4861 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4921 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4981 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5041 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5101 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5161 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5221 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5281 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5341 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5401 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5461 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5521 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5581 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5641 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5701 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5761 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     5821 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     5881 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     5941 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     6001 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     6061 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     6121 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     6181 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     6241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     6421 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6481 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     6541 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     6601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     6661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     6721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     6781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     6841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     6901 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     6961 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     7021 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     7081 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     7141 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     7201 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7261 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7321 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7381 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7441 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7501 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7561 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     7621 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     7681 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     7741 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     7801 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     7861 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgatcacga
     7921 gactagcctc gagggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     7981 gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag atcaaggtta
     8041 ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     8101 ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag
     8161 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     8221 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     8281 aaaagagccc acaacccctc actcggcgcg ccggccacc
//
