LOCUS       sequence-242243-       11382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary plant vector for transient TEV HcPro and fLUC (with intron) 
            expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB118 - pL2_pSB90_fLUC-I_HcPro
FEATURES             Location/Qualifiers
     source          1..11382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        464..809
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2295..2547
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2978..3323
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(3480..3498)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3558..3746
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      5268..5520
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5586..5610
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5890..6519
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6948..8021
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8087..8281
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        8343..8447
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8448..9242
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10567..11155
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11056..11075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    11291..11315
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgatgt atcacctctc gcaaggtttt gacgggttta ctagaattcg agctcggagg
       61 tcaacatggt ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag
      121 aagatcaaag ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat
      181 tccattgccc agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct
      241 acaaatgcca tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg
      301 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca
      361 cgtctacaaa gcaagtggat tgatgtgata acatggtgga gcacgacact ctggtctact
      421 ccaaaaatgt caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa
      481 ggataatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa
      541 ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta
      601 tcattcaaga tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
      661 tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct
      721 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
      781 aaggaagttc atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca
      841 attaccaaca acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa
      901 tgagcgacaa atcaatctct gaggcattct tcataccata ctctaagaaa ttcttggagt
      961 taagaccaga tggaatttcc catgagtgta caagaggagt atcagttgag cggtgcggtg
     1021 aggtggctgc aatcctgaca caagcacttt caccgtgtgg taagatcaca tgcaaacgtt
     1081 gcatggttga aacacctgac attgttgagg gtgagtcggg agacagtgtc accaaccaag
     1141 gtaagctcct agcaatgctg aaagaacagt atccagattt cccaatggcc gagaaactac
     1201 tcacaaggtt tttgcaacag aaatcactag taaatacaaa tttgacagcc tgcgtgagcg
     1261 tcaaacaact cattggtgac cgcaaacaag ctccattcac acacgtactg gctgtcagcg
     1321 aaattctgtt taaaggcaat aaactaacag gggccgatct cgaagaggca agcacacata
     1381 tgcttgaaat agcaaggttc ttgaacaatc gcactgaaaa tatgcgcatt ggccaccttg
     1441 gttctttcag aaataaaatc tcatcgaagg cccatgtgaa taacgcactc atgtgtgata
     1501 atcaacttga tcagaatggg aattttattt ggggactaag gggtgcacac gcaaagaggt
     1561 ttcttaaagg atttttcact gagattgacc caaatgaagg atacgataag tatgttatca
     1621 ggaaacatat caggggtagc agaaagctag caattggcaa tttgataatg tcaactgact
     1681 tccagacgct caggcaacaa attcaaggcg aaactattga gcgtaaagaa attgggaatc
     1741 actgcatttc aatgcggaat ggtaattacg tgtacccatg ttgttgtgtt actcttgaag
     1801 atggtaaggc tcaatattcg gatctaaagc atccaacgaa gagacatctg gtcattggca
     1861 actctggcga ttcaaagtac ctagaccttc cagttctcaa tgaagagaaa atgtatatag
     1921 ctaatgaagg ttattgctac atgaacattt tctttgctct actagtgaat gtcaaggaag
     1981 aggatgcaaa ggacttcacc aagtttataa gggacacaat tgttccaaag cttggagcgt
     2041 ggccaacaat gcaagatgtt gcaactgcat gctacttact ttccattctt tacccagatg
     2101 tcctgagtgc tgaattaccc agaattttgg ttgatcatga caacaaaaca atgcatgttt
     2161 tggattcgta tgggtctaga acgacaggat accacatgtt gaaaatgaac acaacatccc
     2221 agctaattga attcgttcat tcaggtttgg aatccgaaat gaaaactttt aatgttggat
     2281 gagcttgtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     2341 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     2401 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     2461 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     2521 gcgcggtgtc atctatgtta ctagatcgac gctttacgaa ttcccatggg gaggtcaaca
     2581 tggtggagca cgacactctg gtctactcca aaaatgtcaa agatacagtc tcagaagatc
     2641 aaagggctat tgagactttt caacaaagga taatttcggg aaacctcctc ggattccatt
     2701 gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat
     2761 gccatcattg cgataaagga aaggctatca ttcaagatct ctctgccgac agtggtccca
     2821 aagatggacc cccacccacg aggagcatcg tggaaaaaga agaggttcca accacgtcta
     2881 caaagcaagt ggattgatgt gataacatgg tggagcacga cactctggtc tactccaaaa
     2941 atgtcaaaga tacagtctca gaagatcaaa gggctattga gacttttcaa caaaggataa
     3001 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
     3061 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
     3121 aagatctctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     3181 aaaaagaaga ggttccaacc acgtctacaa agcaagtgga ttgatgtgac atctccactg
     3241 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     3301 gttcatttca tttggagagg acacgctcga gtataagagc tcatttttac aacaattacc
     3361 aacaacaaca aacaacaaac aacattacaa ttacatttac aattatcgat acaatggagg
     3421 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc tctagaggat ggaaccgctg
     3481 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
     3541 cagatgcaca tatcgaggta agtttctgct tctacctttg atatatatat aataattatc
     3601 attaattagt agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata
     3661 tagcaattgc ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata
     3721 tatgaccaaa atttgttgat gtgcaggtga acatcacgta cgcggaatac ttcgaaatgt
     3781 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
     3841 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
     3901 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgaaca
     3961 tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
     4021 aaaaaaaatt accaataatc cagaaaatta ttatcatgga ttctaaaacg gattaccagg
     4081 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
     4141 attttgtacc agagtccttt gatcgtgaca aaacaattgc actgataatg aattcctctg
     4201 gatctactgg gttacctaag ggtgtggccc ttccgcatag aactgcctgc gtcagattct
     4261 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
     4321 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
     4381 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tttacgatcc cttcaggatt
     4441 acaaaattca aagtgcgttg ctagtaccaa ccctattttc attcttcgcc aaaagcactc
     4501 tgattgacaa atacgattta tctaatttac acgaaattgc ttctgggggc gcacctcttt
     4561 cgaaagaagt cggggaagcg gttgcaaaac gcttccatct tccagggata cgacaaggat
     4621 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     4681 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     4741 aaacgctggg cgttaatcag agaggcgaat tatgtgtcag aggacctatg attatgtccg
     4801 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     4861 ctggagacat agcttactgg gacgaggacg aacacttctt catagttgac cgcttgaagt
     4921 ctttaattaa atacaaagga tatcaggtgg cccccgctga attggaatcg atattgttac
     4981 aacaccccaa catcttcgac gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac
     5041 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     5101 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     5161 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     5221 taaaggccaa gaagggcgga aagtccaaat tgtaagcttg tcaagcagat cgttcaaaca
     5281 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
     5341 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     5401 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     5461 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     5521 gacgctcaga gaggatgcac atgtgaccga gggacacgaa gtgatccgtt taaactatca
     5581 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
     5641 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgccagccgt
     5701 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
     5761 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
     5821 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
     5881 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
     5941 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
     6001 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
     6061 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
     6121 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
     6181 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
     6241 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
     6301 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
     6361 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
     6421 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
     6481 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
     6541 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
     6601 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
     6661 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
     6721 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
     6781 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
     6841 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc
     6901 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
     6961 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     7021 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     7081 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgt
     7141 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     7201 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     7261 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     7321 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     7381 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     7441 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     7501 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     7561 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     7621 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     7681 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     7741 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     7801 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     7861 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     7921 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
     7981 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
     8041 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaagctt ctttcctgtg gatagcacgt
     8101 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
     8161 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
     8221 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
     8281 aactgtctgg ccagcgcaca gccgaacagc tgcaaaaagc gcccacatca aggctccgag
     8341 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8401 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg gctaaaatga
     8461 gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag
     8521 gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa
     8581 tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc
     8641 tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct
     8701 ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg
     8761 aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca
     8821 tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt
     8881 acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagag gacactccat
     8941 ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg
     9001 tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg
     9061 gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg
     9121 tccggtcgct cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac
     9181 tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt
     9241 agctgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9301 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9361 agttttcgtt ctagctgtaa cctcgaagcg tttcacttgt aacaacgatt gagaattttt
     9421 gtcataaaat tgaaatactt ggttcgcatt tttgtcatcc gcggtcagcc gcaattctga
     9481 cgaactgccc atttagctgg agatgattgt acatccttca cgtgaaaatt tctcaagcgc
     9541 tgtgaacaag ggttcagatt ttagattgaa aggtgagccg ttgaaacacg ttcttcttgt
     9601 cgatgacgac gtcgctatgc ggcatcttat tattgaatac cttacgatcc acgccttcaa
     9661 agtgaccgcg gtagccgaca gcacccagtt cacaagagta ctctcttccg cgacggtcga
     9721 tgtcgtggtt gttgatctag atttaggtcg tgaagatggg ctcgagatcg ttcgtaatct
     9781 ggcggcaaag tctgatattc caatcataat tatcagtggc gaccgccttg aggagacgga
     9841 taaagttgtt gcactcgagc taggagcaag tgattttatc gctaagccgt tcagtatcag
     9901 agagtttcta gcacgcattc gggttgcctt gcgcgtgcgc cccaacgttg tccgctccaa
     9961 agaccgacgg tctttttgtt ttactgactg gacacttaat ctcaggcaac gtcgcttgat
    10021 gtccgaagct ggcggtgagg tgaaacttac ggcaggtgag ttcaatcttc tcctcgcgtt
    10081 tttagagaaa ccccgcgacg ttctatcgcg cgagcaactt ctcattgcca gtcgagtacg
    10141 cgacgaggag gtttatgaca ggagtataga tgttctcatt ttgaggctgc gccgcaaact
    10201 tgaggcggat ccgtcaagcc ctcaactgat aaaaacagca agaggtgccg gttatttctt
    10261 tgacgcggac gtgcaggttt cgcacggggg gacgatggca gcctgagcca attgcatttg
    10321 cctcttaatt atctggctca aagggtgact gaggagtaag cgatgtgccc atcacactga
    10381 ccaccaagac gggggttcgt tttgaagatc acccttcaga cccaaggcca gtgtgcgttg
    10441 atcgcggcca cactgcaaat tcacatactg tcgataaagg ggcgttggac gagaacccat
    10501 tcgtcaggat actggctcgt gatctcactg agcgtcagac cccgtagaaa agatcaaagg
    10561 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    10621 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    10681 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    10741 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    10801 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    10861 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    10921 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    10981 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    11041 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    11101 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    11161 cagcaacgcg gcctttttac ggttcctgct cggatctgtt ggaccggaca gtagtcatgg
    11221 ttgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
    11281 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
    11341 acacattgcg gacgttttta atgtactggg gttgaacact ct
//
