LOCUS       sequence-242244-       10726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary plant vector for transient dual luciferase (rLUC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 10726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB137 -
            pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fL...
FEATURES             Location/Qualifiers
     source          1..10726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        116..295
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    331..386
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     terminator      1440..1692
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(1741..2448)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     intron          3965..4153
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     primer_bind     4213..4231
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    4948..4972
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5252..5881
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6310..7383
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7449..7643
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        7705..7809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7810..8604
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9929..10517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10418..10437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    10653..10677
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 actggggttg aacactctgt gccgaattcg gatccggaga gcggagaatt aagggagtca
       61 cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc
      121 gcaacgttga aggagccact cagccgcggg tttctggagt ttaatgagct aagcacatac
      181 gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat
      241 ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc
      301 tcatattcac tctcatactg tatttttaca acaattacca acaacaacaa acaacaaaca
      361 acattacaat tactatttac aattacaatg acttctaagg tttatgatcc tgaacaaaga
      421 aagagaatga ttactggacc tcaatggtgg gctagatgta agcaaatgaa tgttcttgat
      481 tcttttatta attattatga ttctgaaaag catgctgaaa atgctgttat ttttcttcat
      541 ggaaatgctg cttcttctta tctttggaga catgttgttc cacatattga accagttgct
      601 agatgtatta ttccagatct tattggtatg ggaaagtctg gtaagtctgg taatggttct
      661 tatagacttc ttgatcatta taagtatctt actgcttggt ttgaacttct taatcttcca
      721 aagaagatta tttttgttgg acatgattgg ggtgcttgtt tggcttttca ttattcttat
      781 gaacatcaag ataagattaa ggctattgtt catgctgaat ctgttgttga tgttattgaa
      841 tcttgggatg aatggcctga tattgaagaa gatattgctt tgattaagtc tgaagaagga
      901 gaaaagatgg ttttggaaaa taatttcttt gttgaaacta tgttgccatc taagattatg
      961 agaaagcttg aaccagaaga atttgctgct tatcttgaac catttaagga aaagggtgaa
     1021 gttagaaggc caactctttc ttggcctaga gaaattcctc ttgttaaggg tggtaagcct
     1081 gatgttgttc aaattgttag aaattataat gcttatctta gagcttctga tgatcttcca
     1141 aagatgttta ttgaatctga tccaggattc ttttctaatg ctattgttga aggtgctaag
     1201 aagtttccta atactgaatt tgttaaggta cttcatgctt caacgtgtaa cttaagagat
     1261 actgtgtgaa attttatatt tccatacatt tgcttgacct ttgctttttg tcaatttttt
     1321 tccccttaca ggttaaggga cttcattttt ctcaagaaga tgctcctgat gaaatgggaa
     1381 agtatattaa gtcttttgtt gaaagagttc ttaagaatga acaatgagct tgtcaagcag
     1441 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     1501 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     1561 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     1621 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     1681 tgttactaga tcgacgctgc aaggttttga cgggtttact agaattcgag ctcagcggtc
     1741 ctgctgagcc tcgacatgtt gtcgcaaaat tcgccctgga cccgcccaac gatttgtcgt
     1801 cactgtcaag gtttgacctg cacttcattt ggggcccaca tacaccaaaa aaatgctgca
     1861 taattctcgg ggcagcaagt cggttacccg gccgccgtgc tggaccgggt tgaatggtgc
     1921 ccgtaacttt cggtagagcg gacggccaat actcaacttc aaggaatctc acccatgcgc
     1981 gccggcgggg aaccggagtt cccttcagtg agcgttatta gttcgccgct cggtgtgtcg
     2041 tagatactag cccctggggc acttttgaaa tttgaataag atttatgtaa tcagtctttt
     2101 aggtttgacc ggttctgccg ctttttttaa aattggattt gtaataataa aacgcaattg
     2161 tttgttattg tggcgctcta tcatagatgt cgctataaac ctattcagca caatatattg
     2221 ttttcatttt aatattgtac atataagtag tagggtacaa tcagtaaatt gaacggagaa
     2281 tattattcat aaaaatacga tagtaacggg tgatatattc attagaatga accgaaaccg
     2341 gcggtaagga tctgagctac acatgctcag gttttttaca acgtgcacaa cagaattgaa
     2401 agcaaatatc atgcgatcat aggcttctcg catatctcat taaagcagga caagcttaca
     2461 atttggactt tccgcccttc ttggccttta tgaggatctc tctgattttt cttgcgtcga
     2521 gttttccggt aagacctttc ggtacttcgt ccacaaacac aactcctccg cgcaactttt
     2581 tcgcggttgt tacttgactg gcgacgtaat ccacgatctc tttttccgtc atcgtctttc
     2641 cgtgctccaa aacaacaacg gcggcgggaa gttcaccggc gtcatcgtcg ggcagacctg
     2701 ccacgcccgc gtcgaagatg ttggggtgtt gtaacaatat cgattccaat tcagcggggg
     2761 ccacctgata tcctttgtat ttaattaaag acttcaagcg gtcaactatg aagaagtgtt
     2821 cgtcctcgtc ccagtaagct atgtctccag aatgtagcca tccatccttg tcaatcaagg
     2881 cgttggtcgc ttccggattg tttacataac cggacataat cataggtcct ctgacacata
     2941 attcgcctct ctgattaacg cccagcgttt tcccggtatc cagatccaca accttcgctt
     3001 caaaaaatgg aacaacttta ccgaccgcgc ccggtttatc atccccctcg ggtgtaatca
     3061 gaatagctga tgtagtctca gtgagcccat atccttgtcg tatccctgga agatggaagc
     3121 gttttgcaac cgcttccccg acttctttcg aaagaggtgc gcccccagaa gcaatttcgt
     3181 gtaaattaga taaatcgtat ttgtcaatca gagtgctttt ggcgaagaat gaaaataggg
     3241 ttggtactag caacgcactt tgaattttgt aatcctgaag ggatcgtaaa aacagctctt
     3301 cttcaaatct atacattaag acgactcgaa atccacatat caaatatccg agtgtagtaa
     3361 acattccaaa accgtgatgg aatggaacaa cacttaaaat cgcagtatcc ggaatgattt
     3421 gattgccaaa aataggatct ctggcatgcg agaatctgac gcaggcagtt ctatgcggaa
     3481 gggccacacc cttaggtaac ccagtagatc cagaggaatt cattatcagt gcaattgttt
     3541 tgtcacgatc aaaggactct ggtacaaaat cgtattcatt aaaaccggga ggtagatgag
     3601 atgtgacgaa cgtgtacatc gactgaaatc cctggtaatc cgttttagaa tccatgataa
     3661 taattttctg gattattggt aatttttttt gcacgttcaa aattttttgc aacccctttt
     3721 tggaaacaaa cactacggta ggctgcgaaa tgttcatact gttgagcaat tcacgttcat
     3781 tataaatgtc gttcgcgggc gcaactgcaa ctccgataaa taacgcgccc aacaccggca
     3841 taaagaattg aagagagttt tcactgcata cgacgattct gtgatttgta ttcagcccat
     3901 atcgtttcat agcttctgcc aaccgaacgg acatttcgaa gtattccgcg tacgtgatgt
     3961 tcacctgcac atcaacaaat tttggtcata tattagaaaa gttataaatt aaaatataca
     4021 cacttataaa ctacagaaaa gcaattgcta tatactacat tcttttattt tgaaaaaaat
     4081 atttgaaata ttatattact actaattaat gataattatt atatatatat caaaggtaga
     4141 agcagaaact tacctcgata tgtgcatctg taaaagcaat tgttccagga accagggcgt
     4201 atctcttcat agccttatgc agttgctctc cagcggttcc atcctctaga ggatagaatg
     4261 gcgccgggcc tttctttatg tttttggcgt cctccattgt tgactgtaaa tgatgggagt
     4321 gaagcagaga ggggcagcta tggggtatgg tttgatactt agagggacag tgaaggaata
     4381 taataagccc acaaactcac caaacaaggt ccaagggaaa tcactttcaa agaaggcggt
     4441 ctaagagtga tgtaccacgt ggagtaatat ttataggctt atccaatata cttatgcatt
     4501 cttttattcc tttattttgt atatatcata aatatatttt tatcggttta tacatcaatt
     4561 aataattata attcctaata ctaattattg taaaataatt atatgataaa tagcgattta
     4621 attacaaaat gtttcgcttg tctaattcgt attttcgact atatattaaa atcataatac
     4681 ttttgctaga tctttttgtt tgtccaattt ctgtaatgaa taatattatt gttttaatat
     4741 gataataaat aaattaaact cgtaaaatga tattatagga agattttaaa atttttagta
     4801 gcagaattat ttttttcaaa tagatttttt tttgaaatca gttgtaggga ttaattaatt
     4861 atgaaaatac aaacaataaa gatcctcctt acgaggatgc acatgtgacc gagggacacg
     4921 aagtgatccg tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     4981 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     5041 tccatttgta tgtgccagcc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
     5101 agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
     5161 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
     5221 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
     5281 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
     5341 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
     5401 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
     5461 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
     5521 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
     5581 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
     5641 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
     5701 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
     5761 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
     5821 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
     5881 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
     5941 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
     6001 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
     6061 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
     6121 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
     6181 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
     6241 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga
     6301 ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt
     6361 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca
     6421 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg
     6481 caaccgccgg cagccggtgc gtcgtcgatt aggaagccgc ccaagggcga cgagcaacca
     6541 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac
     6601 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag
     6661 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat
     6721 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa
     6781 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc
     6841 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta
     6901 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg
     6961 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg
     7021 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag
     7081 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt
     7141 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag
     7201 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc
     7261 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct
     7321 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc
     7381 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaagc
     7441 ttctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac
     7501 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat
     7561 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt
     7621 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaca gctgcaaaaa
     7681 gcgcccacat caaggctccg agtgcgcgga acccctattt gtttattttt ctaaatacat
     7741 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7801 aggaagagta tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
     7861 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
     7921 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
     7981 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
     8041 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
     8101 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
     8161 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
     8221 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
     8281 aactgggaag aggacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
     8341 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
     8401 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
     8461 tggtatgaca ttgccttctg cgtccggtcg ctcagggagg atatcgggga agaacagtat
     8521 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
     8581 attttactgg atgaattgtt ttagctgtca gaccaagttt actcatatat actttagatt
     8641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8701 atgaccaaaa tcccttaacg tgagttttcg ttctagctgt aacctcgaag cgtttcactt
     8761 gtaacaacga ttgagaattt ttgtcataaa attgaaatac ttggttcgca tttttgtcat
     8821 ccgcggtcag ccgcaattct gacgaactgc ccatttagct ggagatgatt gtacatcctt
     8881 cacgtgaaaa tttctcaagc gctgtgaaca agggttcaga ttttagattg aaaggtgagc
     8941 cgttgaaaca cgttcttctt gtcgatgacg acgtcgctat gcggcatctt attattgaat
     9001 accttacgat ccacgccttc aaagtgaccg cggtagccga cagcacccag ttcacaagag
     9061 tactctcttc cgcgacggtc gatgtcgtgg ttgttgatct agatttaggt cgtgaagatg
     9121 ggctcgagat cgttcgtaat ctggcggcaa agtctgatat tccaatcata attatcagtg
     9181 gcgaccgcct tgaggagacg gataaagttg ttgcactcga gctaggagca agtgatttta
     9241 tcgctaagcc gttcagtatc agagagtttc tagcacgcat tcgggttgcc ttgcgcgtgc
     9301 gccccaacgt tgtccgctcc aaagaccgac ggtctttttg ttttactgac tggacactta
     9361 atctcaggca acgtcgcttg atgtccgaag ctggcggtga ggtgaaactt acggcaggtg
     9421 agttcaatct tctcctcgcg tttttagaga aaccccgcga cgttctatcg cgcgagcaac
     9481 ttctcattgc cagtcgagta cgcgacgagg aggtttatga caggagtata gatgttctca
     9541 ttttgaggct gcgccgcaaa cttgaggcgg atccgtcaag ccctcaactg ataaaaacag
     9601 caagaggtgc cggttatttc tttgacgcgg acgtgcaggt ttcgcacggg gggacgatgg
     9661 cagcctgagc caattgcatt tgcctcttaa ttatctggct caaagggtga ctgaggagta
     9721 agcgatgtgc ccatcacact gaccaccaag acgggggttc gttttgaaga tcacccttca
     9781 gacccaaggc cagtgtgcgt tgatcgcggc cacactgcaa attcacatac tgtcgataaa
     9841 ggggcgttgg acgagaaccc attcgtcagg atactggctc gtgatctcac tgagcgtcag
     9901 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9961 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10021 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    10081 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10141 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10201 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10261 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10321 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10381 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10441 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10501 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg ctcggatctg
    10561 ttggaccgga cagtagtcat ggttgatggg ctgcctgtat cgagtggtga ttttgtgccg
    10621 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
    10681 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgt
//
