LOCUS       sequence-242249-        6054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary plant vector for transient expression of a Renilla luciferase
            (rLUC, with intron) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB165 - pL1R3_pMAS::rLUC-I::tMAS
FEATURES             Location/Qualifiers
     source          1..6054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      137..389
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    498..522
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      614..1245
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1332..1436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1437..1505,1506..2297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1655..1674)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2468..3056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2957..2976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3392..4540
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    4696..4720
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        4768..5148
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
ORIGIN
        1 cttgaccttt gctttttgtc aatttttttc cccttacagg ttaagggact tcatttttct
       61 caagaagatg ctcctgatga aatgggaaag tatattaagt cttttgttga aagagttctt
      121 aagaatgaac aatgagcttg gactcccatg ttggcaaagg caaccaaaca aacaatgaat
      181 gatccgctcc tgcatatggg gcggtttgag tatttcaact gccatttggg ctgaattgta
      241 gacatgctcc tgtcagaaat tccgtgatct tactcaatat tcagtaatct cggccaatat
      301 cctaaatgtg cgtggcttta tctgtctttg tattgtttca tcaattcatg taacgtttgc
      361 ttttcttatg aattttcaaa taaattatcc gctgagctcg aattctagtt tgtcttcaca
      421 gagtggggcc cactgcatcc accccagtac attaaaaacg tccgcaatgt gttattaagt
      481 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
      541 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtcagatca
      601 ggatctcctt tgcgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct
      661 atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc
      721 ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga cagatgaggg gcggacgttg
      781 acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag gctcgatttc
      841 ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga ttttacgcga
      901 gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat tgacacttga
      961 ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg cagagtgctg
     1021 acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga aaatccagca
     1081 tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa cctgctttta
     1141 aaccaatatt tataaacctt gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca
     1201 cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc gggcctccca
     1261 tccccccagg ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg
     1321 gccaattcgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1381 cgctcatgag acaataacct tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1441 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1501 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1561 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1621 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     1681 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1741 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1801 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1861 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1921 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1981 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2041 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2101 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt ggttctcgcg
     2161 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2221 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2281 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2341 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2401 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2461 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2521 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2581 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2641 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2701 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2761 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2821 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2881 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2941 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3001 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3061 ccagcaacgc ggccttttta cggttcctgg cagatcctag atgtggcgca acgatgccgg
     3121 cgacaagcag gagcgcaccg acttcttccg catcaagtgt tttggctctc aggccgaggc
     3181 ccacggcaag tatttgggca aggggtcgct ggtattcgtg cagggcaaga ttcggaatac
     3241 caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg ataaggtgga
     3301 ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca ttgccccggc
     3361 gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc ggaaggcata
     3421 caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca tcgcaagccg
     3481 caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc
     3541 tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc ccgcgccatc
     3601 ggccgccgtg gagcgttcgc gtcgtcttga acaggaggcg gcaggtttgg cgaagtcgat
     3661 gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg gcgaggacct
     3721 ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga agcagcagat
     3781 caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat
     3841 gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga
     3901 ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca cctacaccgg
     3961 cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg agtacgcgaa
     4021 gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc aggacctggg
     4081 ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc
     4141 gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca
     4201 ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga
     4261 ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg agaagtaccg
     4321 caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc gggagccgta
     4381 cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa
     4441 gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc tggtggaaca
     4501 cgcctgggtc aatgatgacc tggtgcattg caaacgctag ggccttgtgg ggtcagttcc
     4561 ggctgggggt tcagcagcca gcgcctgatc tggggaaccc tgtggttggc atgcacatac
     4621 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     4681 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaacca
     4741 cttcgtgcag aagacaagta aggagatttt tcaaatcagt gcgcaagacg tgacgtaagt
     4801 atccgagtca gtttttattt ttctactaat ttggtcgttt atttcggcgt gtaggacatg
     4861 gcaaccgggc ctgaatttcg cgggtattct gtttctattc caactttttc ttgatccgca
     4921 gccattaacg acttttgaat agatacgctg acacgccaag cctcgctagt caaaagtgta
     4981 ccaaacaacg ctttacagca agaacggaat gcgcgtgacg ctcgcggtga cgccatttcg
     5041 ccttttcaga aatggataaa tagccttgct tcctattata tcttcccaaa ttaccaatac
     5101 attacactag catctgaatt tcataaccaa tctcgataca ccaaatcgaa tgacttctaa
     5161 ggtttatgat cctgaacaaa gaaagagaat gattactgga cctcaatggt gggctagatg
     5221 taagcaaatg aatgttcttg attcttttat taattattat gattctgaaa agcatgctga
     5281 aaatgctgtt atttttcttc atggaaatgc tgcttcttct tatctttgga gacatgttgt
     5341 tccacatatt gaaccagttg ctagatgtat tattccagat cttattggta tgggaaagtc
     5401 tggtaagtct ggtaatggtt cttatagact tcttgatcat tataagtatc ttactgcttg
     5461 gtttgaactt cttaatcttc caaagaagat tatttttgtt ggacatgatt ggggtgcttg
     5521 tttggctttt cattattctt atgaacatca agataagatt aaggctattg ttcatgctga
     5581 atctgttgtt gatgttattg aatcttggga tgaatggcct gatattgaag aagatattgc
     5641 tttgattaag tctgaagaag gagaaaagat ggttttggaa aataatttct ttgttgaaac
     5701 tatgttgcca tctaagatta tgagaaagct tgaaccagaa gaatttgctg cttatcttga
     5761 accatttaag gaaaagggtg aagttagaag gccaactctt tcttggccta gagaaattcc
     5821 tcttgttaag ggtggtaagc ctgatgttgt tcaaattgtt agaaattata atgcttatct
     5881 tagagcttct gatgatcttc caaagatgtt tattgaatct gatccaggat tcttttctaa
     5941 tgctattgtt gaaggtgcta agaagtttcc taatactgaa tttgttaagg tacttcatgc
     6001 ttcaacgtgt aacttaagag atactgtgtg aaattttata tttccataca tttg
//
