LOCUS       sequence-242251-       10551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary plant vector for transient expression of a Renilla luciferase
            (rLUC, with intron) and a firefly luciferase (fLUC, with intron) in 
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 10551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB167 -
            pL2_pSB90_2x35S::fLUC-I::tNOS_tMAS::r...
FEATURES             Location/Qualifiers
     source          1..10551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    42..66
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        132..512
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     terminator      1555..1807
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     terminator      complement(1853..2105)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          3627..3815
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     primer_bind     3875..3893
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        complement(4050..4395)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(4087..4107)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    4888..4912
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      complement(5048..5636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5128..5147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6961..7755)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(7756..7860)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7922..8116
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8182..9255)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9684..10313)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc
       61 ctgtcaaaca ctgatagttt aaacggatca cttcgtgtcc ctcggtcaca tgtgcatcct
      121 cgtaaggaga tttttcaaat cagtgcgcaa gacgtgacgt aagtatccga gtcagttttt
      181 atttttctac taatttggtc gtttatttcg gcgtgtagga catggcaacc gggcctgaat
      241 ttcgcgggta ttctgtttct attccaactt tttcttgatc cgcagccatt aacgactttt
      301 gaatagatac gctgacacgc caagcctcgc tagtcaaaag tgtaccaaac aacgctttac
      361 agcaagaacg gaatgcgcgt gacgctcgcg gtgacgccat ttcgcctttt cagaaatgga
      421 taaatagcct tgcttcctat tatatcttcc caaattacca atacattaca ctagcatctg
      481 aatttcataa ccaatctcga tacaccaaat cgaatgactt ctaaggttta tgatcctgaa
      541 caaagaaaga gaatgattac tggacctcaa tggtgggcta gatgtaagca aatgaatgtt
      601 cttgattctt ttattaatta ttatgattct gaaaagcatg ctgaaaatgc tgttattttt
      661 cttcatggaa atgctgcttc ttcttatctt tggagacatg ttgttccaca tattgaacca
      721 gttgctagat gtattattcc agatcttatt ggtatgggaa agtctggtaa gtctggtaat
      781 ggttcttata gacttcttga tcattataag tatcttactg cttggtttga acttcttaat
      841 cttccaaaga agattatttt tgttggacat gattggggtg cttgtttggc ttttcattat
      901 tcttatgaac atcaagataa gattaaggct attgttcatg ctgaatctgt tgttgatgtt
      961 attgaatctt gggatgaatg gcctgatatt gaagaagata ttgctttgat taagtctgaa
     1021 gaaggagaaa agatggtttt ggaaaataat ttctttgttg aaactatgtt gccatctaag
     1081 attatgagaa agcttgaacc agaagaattt gctgcttatc ttgaaccatt taaggaaaag
     1141 ggtgaagtta gaaggccaac tctttcttgg cctagagaaa ttcctcttgt taagggtggt
     1201 aagcctgatg ttgttcaaat tgttagaaat tataatgctt atcttagagc ttctgatgat
     1261 cttccaaaga tgtttattga atctgatcca ggattctttt ctaatgctat tgttgaaggt
     1321 gctaagaagt ttcctaatac tgaatttgtt aaggtacttc atgcttcaac gtgtaactta
     1381 agagatactg tgtgaaattt tatatttcca tacatttgct tgacctttgc tttttgtcaa
     1441 tttttttccc cttacaggtt aagggacttc atttttctca agaagatgct cctgatgaaa
     1501 tgggaaagta tattaagtct tttgttgaaa gagttcttaa gaatgaacaa tgagcttgga
     1561 ctcccatgtt ggcaaaggca accaaacaaa caatgaatga tccgctcctg catatggggc
     1621 ggtttgagta tttcaactgc catttgggct gaattgtaga catgctcctg tcagaaattc
     1681 cgtgatctta ctcaatattc agtaatctcg gccaatatcc taaatgtgcg tggctttatc
     1741 tgtctttgta ttgtttcatc aattcatgta acgtttgctt ttcttatgaa ttttcaaata
     1801 aattatccgc tgagctcgaa ttctagtaaa cccgtcaaaa ccttgcagcg tcgatctagt
     1861 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
     1921 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
     1981 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
     2041 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
     2101 cgatctgctt gacaagctta caatttggac tttccgccct tcttggcctt tatgaggatc
     2161 tctctgattt ttcttgcgtc gagttttccg gtaagacctt tcggtacttc gtccacaaac
     2221 acaactcctc cgcgcaactt tttcgcggtt gttacttgac tggcgacgta atccacgatc
     2281 tctttttccg tcatcgtctt tccgtgctcc aaaacaacaa cggcggcggg aagttcaccg
     2341 gcgtcatcgt cgggcagacc tgccacgccc gcgtcgaaga tgttggggtg ttgtaacaat
     2401 atcgattcca attcagcggg ggccacctga tatcctttgt atttaattaa agacttcaag
     2461 cggtcaacta tgaagaagtg ttcgtcctcg tcccagtaag ctatgtctcc agaatgtagc
     2521 catccatcct tgtcaatcaa ggcgttggtc gcttccggat tgtttacata accggacata
     2581 atcataggtc ctctgacaca taattcgcct ctctgattaa cgcccagcgt tttcccggta
     2641 tccagatcca caaccttcgc ttcaaaaaat ggaacaactt taccgaccgc gcccggttta
     2701 tcatccccct cgggtgtaat cagaatagct gatgtagtct cagtgagccc atatccttgt
     2761 cgtatccctg gaagatggaa gcgttttgca accgcttccc cgacttcttt cgaaagaggt
     2821 gcgcccccag aagcaatttc gtgtaaatta gataaatcgt atttgtcaat cagagtgctt
     2881 ttggcgaaga atgaaaatag ggttggtact agcaacgcac tttgaatttt gtaatcctga
     2941 agggatcgta aaaacagctc ttcttcaaat ctatacatta agacgactcg aaatccacat
     3001 atcaaatatc cgagtgtagt aaacattcca aaaccgtgat ggaatggaac aacacttaaa
     3061 atcgcagtat ccggaatgat ttgattgcca aaaataggat ctctggcatg cgagaatctg
     3121 acgcaggcag ttctatgcgg aagggccaca cccttaggta acccagtaga tccagaggaa
     3181 ttcattatca gtgcaattgt tttgtcacga tcaaaggact ctggtacaaa atcgtattca
     3241 ttaaaaccgg gaggtagatg agatgtgacg aacgtgtaca tcgactgaaa tccctggtaa
     3301 tccgttttag aatccatgat aataattttc tggattattg gtaatttttt ttgcacgttc
     3361 aaaatttttt gcaacccctt tttggaaaca aacactacgg taggctgcga aatgttcata
     3421 ctgttgagca attcacgttc attataaatg tcgttcgcgg gcgcaactgc aactccgata
     3481 aataacgcgc ccaacaccgg cataaagaat tgaagagagt tttcactgca tacgacgatt
     3541 ctgtgatttg tattcagccc atatcgtttc atagcttctg ccaaccgaac ggacatttcg
     3601 aagtattccg cgtacgtgat gttcacctgc acatcaacaa attttggtca tatattagaa
     3661 aagttataaa ttaaaatata cacacttata aactacagaa aagcaattgc tatatactac
     3721 attcttttat tttgaaaaaa atatttgaaa tattatatta ctactaatta atgataatta
     3781 ttatatatat atcaaaggta gaagcagaaa cttacctcga tatgtgcatc tgtaaaagca
     3841 attgttccag gaaccagggc gtatctcttc atagccttat gcagttgctc tccagcggtt
     3901 ccatcctcta gaggatagaa tggcgccggg cctttcttta tgtttttggc gtcctccatt
     3961 gtatcgataa ttgtaaatgt aattgtaatg ttgtttgttg tttgttgttg ttggtaattg
     4021 ttgtaaaaat gagctcttat actcgagcgt gtcctctcca aatgaaatga acttccttat
     4081 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     4141 atgtcacatc aatccacttg ctttgtagac gtggttggaa cctcttcttt ttccacgatg
     4201 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagagagatc ttgaatgata
     4261 gcctttcctt tatcgcaatg atggcatttg taggagccac cttccttttc tactgtcctt
     4321 tcgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga aattatcctt
     4381 tgttgaaaag tctcaatagc cctttgatct tctgagactg tatctttgac atttttggag
     4441 tagaccagag tgtcgtgctc caccatgtta tcacatcaat ccacttgctt tgtagacgtg
     4501 gttggaacct cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
     4561 ctgtcggcag agagatcttg aatgatagcc tttcctttat cgcaatgatg gcatttgtag
     4621 gagccacctt ccttttctac tgtcctttcg atgaagtgac agatagctgg gcaatggaat
     4681 ccgaggaggt ttcccgaaat tatcctttgt tgaaaagtct caatagccct ttgatcttct
     4741 gagactgtat ctttgacatt tttggagtag accagagtgt cgtgctccac catgttgacc
     4801 tccggatccg aattcggcac agagtgttca accccagtac attaaaaacg tccgcaatgt
     4861 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag
     4921 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
     4981 aaccatgact actgtccggt ccaacagatc cgagcaggaa ccgtaaaaag gccgcgttgc
     5041 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5101 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5161 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5221 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5281 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5341 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5401 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5461 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     5521 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5581 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5641 atcctttgat cttttctacg gggtctgacg ctcagtgaga tcacgagcca gtatcctgac
     5701 gaatgggttc tcgtccaacg cccctttatc gacagtatgt gaatttgcag tgtggccgcg
     5761 atcaacgcac actggccttg ggtctgaagg gtgatcttca aaacgaaccc ccgtcttggt
     5821 ggtcagtgtg atgggcacat cgcttactcc tcagtcaccc tttgagccag ataattaaga
     5881 ggcaaatgca attggctcag gctgccatcg tccccccgtg cgaaacctgc acgtccgcgt
     5941 caaagaaata accggcacct cttgctgttt ttatcagttg agggcttgac ggatccgcct
     6001 caagtttgcg gcgcagcctc aaaatgagaa catctatact cctgtcataa acctcctcgt
     6061 cgcgtactcg actggcaatg agaagttgct cgcgcgatag aacgtcgcgg ggtttctcta
     6121 aaaacgcgag gagaagattg aactcacctg ccgtaagttt cacctcaccg ccagcttcgg
     6181 acatcaagcg acgttgcctg agattaagtg tccagtcagt aaaacaaaaa gaccgtcggt
     6241 ctttggagcg gacaacgttg gggcgcacgc gcaaggcaac ccgaatgcgt gctagaaact
     6301 ctctgatact gaacggctta gcgataaaat cacttgctcc tagctcgagt gcaacaactt
     6361 tatccgtctc ctcaaggcgg tcgccactga taattatgat tggaatatca gactttgccg
     6421 ccagattacg aacgatctcg agcccatctt cacgacctaa atctagatca acaaccacga
     6481 catcgaccgt cgcggaagag agtactcttg tgaactgggt gctgtcggct accgcggtca
     6541 ctttgaaggc gtggatcgta aggtattcaa taataagatg ccgcatagcg acgtcgtcat
     6601 cgacaagaag aacgtgtttc aacggctcac ctttcaatct aaaatctgaa cccttgttca
     6661 cagcgcttga gaaattttca cgtgaaggat gtacaatcat ctccagctaa atgggcagtt
     6721 cgtcagaatt gcggctgacc gcggatgaca aaaatgcgaa ccaagtattt caattttatg
     6781 acaaaaattc tcaatcgttg ttacaagtga aacgcttcga ggttacagct agaacgaaaa
     6841 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6901 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6961 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
     7021 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgagcgaccg
     7081 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
     7141 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
     7201 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
     7261 aaatggagtg tcctcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
     7321 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
     7381 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
     7441 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
     7501 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
     7561 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
     7621 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
     7681 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
     7741 tctcatttta gccatactct tcctttttca atattattga agcatttatc agggttattg
     7801 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7861 cactcggagc cttgatgtgg gcgctttttg cagctgttcg gctgtgcgct ggccagacag
     7921 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
     7981 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
     8041 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
     8101 gtacgtgcta tccacaggaa agaagctttt tcgacctttt tcccctgcta gggcaatttg
     8161 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
     8221 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
     8281 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
     8341 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
     8401 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
     8461 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
     8521 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
     8581 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
     8641 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
     8701 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
     8761 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
     8821 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
     8881 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
     8941 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
     9001 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
     9061 cgacgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
     9121 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
     9181 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
     9241 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     9301 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     9361 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     9421 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     9481 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     9541 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     9601 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     9661 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     9721 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     9781 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     9841 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     9901 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     9961 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
    10021 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
    10081 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
    10141 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
    10201 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
    10261 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
    10321 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
    10381 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
    10441 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
    10501 gcacggctgg cacatacaaa tggacgaacg gataaacctt ttcacgccct t
//
