LOCUS       sequence-242275-        3759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for anti-sense in situ probe in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Castro JP, Yancoskie MN, Marchini M, Belohlavy S, Hiramatsu L, Kucka
            M, Beluch WH, Naumann R, Skuplik I, Cobb J, Barton NH, Rolian C, 
            Chan YF
  TITLE     An integrative genomic analysis of the Longshanks selection 
            experiment for longer limbs in mice.
  JOURNAL   Elife. 2019 Jun 6;8:e42014. doi: 10.7554/eLife.42014.
  PUBMED    31169497
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-06-6"; volume: "8:e42014. doi"; pages: " 
            10.7554/eLife.42014"
COMMENT     Sequence Label: pJET1.2_Rab28_Anti-Sense
FEATURES             Location/Qualifiers
     source          1..3759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(1287..1293,1294..1311,1312..1317))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1332..1353
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1473..1490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1644..2232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1724..1743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2403..3194,3195..3263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3026..3045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3264..3368)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3436..3454
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 taatacgact cactataggg agagcggccg ccagatcttc cggatggctc gagtttttca
       61 gcaagatgat catcaaagct tggagcagct tcatatctgg ttaaattttt tatatctggg
      121 aaaccaagaa gtgaagagag aaggaagcaa catggttctt ctggagtgag gaggcagtct
      181 caatccagag aatggactct ccattcggat ttcaaaacgt gtggttccaa atcgcactcc
      241 ttccggtcca gggagctgcc tgatccctca ggcaactaaa ggatgaagga gcagagcagg
      301 aggaagtgtg agctggctac ggagacagtc actgataaaa cacgttccta ggcagctggg
      361 gcccaattca ggggaagagc agccagaact atcaatacag tgtgtgctca ctgcactgca
      421 cacatggagc tccgaggagg gttaacagtt cttgacaagg gctcctggtt gtagtttaca
      481 atatctgcct tcaccactct ctgtgactgt tctatttctg ctttgtttaa tttgattccc
      541 aggatttctg ctgcaacttt ctgaaaacac aggaagacag agtctcctgt cttggctgaa
      601 acaaagtggc tactaaaacc attttcctgg caaaatcgta agtgcttatc agcttttact
      661 gttcgcatat gctccaagtc aattttattg cccactaaag caaccagggg ctgagtttct
      721 gactcttcac ttactgtctt caccacagaa taccaatctt ccaaattctc aaagctttgg
      781 tagtttgtaa tatcatatac caagaggatt ccctgtgctc catagatgta tttatccaac
      841 atcttgccac ctatctttct agaagatctc ctacaatatt ctcagctgcc atggaaaatc
      901 gatgttcttc ttttattctc tcaagatttt caggctgtat attaaaactt atattaagaa
      961 ctatgctaac cacctcatca ggaaccgttg taggtggcgt gggttttctt ggcaatcgac
     1021 tctcatgaaa actacgagct aaatattcaa tatgttcctc ttgaccaact ttattctgca
     1081 ttttttttga acgaggttta gagcaagctt caggaaactg agacaggaat tttattaaaa
     1141 atttaaattt tgaagaaagt tcagggttaa tagcatccat tttttgcttt gcaagttcct
     1201 cagcattctt aacaaaagac gtctcttttg acatgtttaa agtttaaacc tcctgtgtga
     1261 aattattatc cgctcataat tccacacatt atacgagccg gaagcataaa gtgtaaagcc
     1321 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gccaattgct
     1381 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1441 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1501 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1621 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2041 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2281 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2341 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2401 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2461 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2521 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2581 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2641 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2701 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2761 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2821 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2881 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2941 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3001 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3061 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3361 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3421 acattaacct ataaaaatag gcgtatcacg aggccgcccc tgcagccgaa ttatattatt
     3481 tttgccaaat aatttttaac aaaagctctg aagtcttctt catttaaatt cttagatgat
     3541 acttcatctg gaaaattgtc ccaattagta gcatcacgct gtgagtaagt tctaaaccat
     3601 ttttttattg ttgtattatc tctaatctta ctactcgatg agttttcggt attatctcta
     3661 tttttaactt ggagcaggtt ccattcattg tttttttcat catagtgaat aaaatcaact
     3721 gctttaacac ttgtgcctga acaccatatc catccggcg
//
